Kit for preparing sample for detecting monoclonal antibody
US-2018052172-A1 · Feb 22, 2018 · US
Method for detecting monoclonal antibody using mass spectrometry
US10338039B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10338039-B2 |
| Application number | US-201515556018-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 18, 2015 |
| Priority date | Mar 9, 2015 |
| Publication date | Jul 2, 2019 |
| Grant date | Jul 2, 2019 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
A method is provided for more easily detecting and quantifying a protein by regioselectively digesting a variable region of an Fab domain of a monoclonal antibody while suppressing proteolysis of an Fc domain. In the method, a porous body in which a monoclonal antibody is immobilized in pores and nanoparticles on which a protease is immobilized are brought into contact with each other in a liquid to perform selective proteolysis of the monoclonal antibody, and a resulting peptide fragment is detected using liquid chromatography-mass spectrometry (LC-MS), and a peptide having an amino acid sequence that includes an amino acid derived from a CDR2 region of a heavy chain or a light chain of the monoclonal antibody is detected.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of detecting a monoclonal antibody, comprising: bringing into contact in a liquid a porous body having pores in which a monoclonal antibody is immobilized and nanoparticles on which a protease is immobilized such that a selective proteolysis of the monoclonal antibody occurs; and detecting a peptide fragment by multiple reaction monitoring under conditions described below using liquid chromatography-mass spectrometry, Trastuzumab or trastuzumab-DM1 quantitation peptide and its mass spectrometry information Reten- Q1 Colli- Q3 Parent Frag- tion Pre sion Pre ion ment time Bias Energy Bias Peptide Sequence m/z ion m/z [minutes] [V] [V] [V] IYPTNGYTR 542.8 808.4 3.405 −40 −18 −30 (SEQ ID NO. 1) IYPTNGYTR 542.8 711.3 3.405 −40 −26 −38 (SEQ ID NO. 1) IYPTNGYTR 542.8 610.3 3.405 −40 −24 −22 (SEQ ID NO. 1) IYPTNGYTR 542.8 404.7 3.405 −20 −18 −15 (SEQ ID NO. 1) IYPTNGYTR 542.8 277.2 3.405 −20 −16 −30 (SEQ ID NO. 1) FTISADTSK 485.2 822.4 3.635 −18 −18 −32 (SEQ ID NO. 3) FTISADTSK 485.2 721.4 3.635 −11 −18 −26 (SEQ ID NO. 3) FTISADTSK 485.2 608.3 3.635 −18 −19 −32 (SEQ ID NO. 3) FTISADTSK 485.2 521.3 3.635 −18 −19 −38 (SEQ ID NO. 3) FTISADTSK 485.2 335.2 3.635 −18 −24 −24 (SEQ ID NO. 3) GLEWVAR 415.7 660.3 4.077 −30 −15 −24 (SEQ ID NO. 6) GLEWVAR 415.7 531.3 4.077 −30 −16 −40 (SEQ ID NO. 6) GLEWVAR 415.7 345.2 4.077 −16 −17 −24 (SEQ ID NO. 6) GLEWVAR 415.7 246.2 4.077 −30 −18 −17 (SEQ ID NO. 6) NTAYLQMNSLR 655.8 1095.6 4.144 −24 −23 −42 (SEQ ID NO. 7) NTAYLQMNSLR 655.8 1024.5 4.144 −24 −22 −40 (SEQ ID NO. 7) NTAYLQMNSLR 655.8 861.5 4.144 −24 −22
Assignees
Inventors
Classifications
Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 · CPC title
involving amino acids · CPC title
Antibody fragments · CPC title
involving peptides or proteins · CPC title
Preparation · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US10338039B2 cover?
- A method is provided for more easily detecting and quantifying a protein by regioselectively digesting a variable region of an Fab domain of a monoclonal antibody while suppressing proteolysis of an Fc domain. In the method, a porous body in which a monoclonal antibody is immobilized in pores and nanoparticles on which a protease is immobilized are brought into contact with each other in …
- Who is the assignee on this patent?
- Shimadzu Corp
- What technology area does this patent fall under?
- Primary CPC classification G01N30/7233. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Jul 02 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).