Compositions for producing glucose syrups

Patent metadata

Field	Value
Publication number	US-10337041-B2
Application number	US-201515116006-A
Country	US
Kind code	B2
Filing date	Feb 6, 2015
Priority date	Feb 7, 2014
Publication date	Jul 2, 2019
Grant date	Jul 2, 2019

Abstract

The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.

First claim

The invention claimed is:

1. A composition comprising an alpha-amylase, a pullulanase and a glucoamylase, wherein
the alpha-amylase has at least 90% sequence identity to SEQ ID NO: 5 or a mature polypeptide thereof,
the pullulanase has at least 90% sequence identity to SEQ ID NO: 16 or a mature polypeptide thereof,
the glucoamylase has at least 90% sequence identity to SEQ ID NO: 4 or a mature polypeptide thereof, and
the ratio of pullulanase dose in New Pullulanase Units Novozymes (NPUN)/gDS to alpha-amylase dose in Acid Fungal Alpha-amylase Units (FAU(A))/gDS is at least 60 and the ratio of pullulanase dose in New Pullulanase Units Novozymes (NPUN)/gDS to glucoamylase dose in Glucoamylase Units (AGU)/gDS is in the range of 2-15.

2. The composition of claim 1 , wherein the ratio of pullulanase dose in NPUN/gDS to alpha-amylase dose in FAU(A)/gDS is in the range of 100-700.

3. The composition of claim 1 , wherein the ratio of pullulanase dose in NPUN/gDS to alpha-amylase dose in FAU(A)/gDS is in the range of 200-600.

4. The composition of claim 1 , wherein the ratio of pullulanase dose in NPUN/gDS to alpha-amylase dose in FAU(A)/gDS is in the range of 300-500.

5. The composition of claim 1 , wherein the ratio of pullulanase dose in NPUN/gDS to alpha-amylase dose in FAU(A)/gDS is in the range of 350-500.

6. The composition of claim 1 , wherein the ratio of pullulanase dose in NPUN/gDS to alpha-amylase dose in FAU(A)/gDS is in the range of 375-475.

7. The composition of claim 1 , wherein the ratio of pullulanase dose in NPUN/gDS to alpha-amylase dose in FAU(A)/gDS is in the range of 400-450.

8. The composition of claim 1 , wherein the ratio of pullulanase dose in NPUN/gDS to glucoamylase dose in AGU/gDS is in the range of 2-10.

9. The composition of claim 1 , wherein the ratio of pullulanase dose in NPUN/gDS to glucoamylase dose in AGU/gDS is in the range of 2-5.

10. The composition of claim 1 , wherein the ratio of pullulanase dose in NPUN/gDS to glucoamylase dose in AGU/gDS is in the range of 3-5.

11. The composition of claim 1 , wherein the ratio of pullulanase dose in NPUN/gDS to glucoamylase dose in AGU/gDS is in the range of 3.5-4.

12. The composition of claim 1 , wherein the composition comprises an alpha-amylase of SEQ ID NO: 5, a pullulanase of SEQ ID NO: 16 and a glucoamylase of SEQ ID NO: 4.

13. A method of making glucose syrup, comprising contacting liquefied starch with a composition of claim 1 .

14. The method of claim 13 , wherein the initial dry solids content (DS) in the liquefied starch is at least 25%.

15. The method of claim 13 , wherein contacting time is at least 24 hours.

Assignees

• Novozymes As

Inventors

• Fukuyama Shiro
• Ayabe Keiichi

Classifications

• C13K1/06

  obtained by saccharification of starch or raw materials containing starch · CPC title

• A23L29/35

  Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12N9/2457

  Pullulanase (3.2.1.41) · CPC title

• C12Y302/01001

  Alpha-amylase (3.2.1.1) · CPC title