Isolated polynucleotides and polypeptides, and methods of using same for increasing plant yield and/or agricultural characteristics

Patent metadata

Field	Value
Publication number	US-10337023-B2
Application number	US-201815950222-A
Country	US
Kind code	B2
Filing date	Apr 11, 2018
Priority date	Aug 27, 2013
Publication date	Jul 2, 2019
Grant date	Jul 2, 2019

Abstract

Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 710-1153 and 9276-15726, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-709 and 1157-9275, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.

First claim

What is claimed is:

1. A method of increasing seed yield, growth rate, and/or biomass of a plant, the method comprising:
(a) transforming plants with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 1011, and,
(b) selecting a plant from step (a) which is transformed with said exogenous polynucleotide, overexpressing said polypeptide and exhibiting an increased dry weight, leaf blade area, leaf number, plot coverage, relative growth rate of leaf number, relative growth rate of plot coverage, relative growth rate of rosette diameter, harvest index, seed yield, rosette area, or rosette diameter as compared to a wild type plant of the same species which is grown under the same growth conditions, thereby increasing the seed yield, growth rate, and/or biomass of the plant.

2. The method of claim 1 , wherein said selected transformed plant further exhibits a reduced time to flowering or a reduced time to inflorescence emergence as compared to a wild type plant of the same species which is grown under the same growth conditions.

3. A method of producing a plant crop comprising:
(a) growing plants of a crop transformed with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 1011,
(b) selecting a crop plant from step (a) which is transformed with said exogenous polynucleotide, overexpressing said polypeptide and exhibiting an increased dry weight, leaf blade area, leaf number, plot coverage, relative growth rate of leaf number, relative growth rate of plot coverage, relative growth rate of rosette diameter, harvest index, rosette area, or rosette diameter as compared to a wild type plant of the same species which is grown under the same growth conditions, and,
(c) breeding selected transformed crop plant of step (b) to produce said plant crop having crop plants transformed with said exogenous polynucleotide and exhibiting said increased dry weight, leaf blade area, leaf number, plot coverage, relative growth rate of leaf number, relative growth rate of plot coverage, relative growth rate of rosette diameter, harvest index, rosette area, or rosette diameter as compared to as compared to a wild type plant of the same species which is grown under the same growth conditions, thereby producing the plant crop.

4. The method of claim 3 , wherein said selected transformed plant further exhibits a reduced time to flowering or a reduced time to inflorescence emergence as compared to a wild type plant of the same species which is grown under the same growth conditions.

5. The method of claim 3 , wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 686 and 302.

6. A method of growing a plant crop, the method comprising:
(a) seeding seeds and/or planting plantlets of plants of a crop transformed with a nucleic acid construct comprising an isolated polynucleotide which comprises a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 1011, and wherein said isolated polynucleotide is operably linked to a heterologous promoter for directing transcription of said nucleic acid sequence in a plant cell,
(b) selecting a crop plant from step (a) which is transformed with said nucleic acid construct, overexpressing said polypeptide and exhibiting at least one trait selected from the group consisting of increased dry weight, increased leaf blade area, increased leaf number, increased plot coverage, increased relative growth rate of leaf number, increased relative growth rate of plot coverage, increased relative growth rate of rosette diameter, increased harvest index, increased rosette area, increased rosette diameter, reduced time to flowering and reduced time to inflorescence emergence as compared to a wild type plant of the same species which is grown under the same growth conditions, and
(c) breeding selected transformed crop plant of step (b) to produce said plant crop having crop plants transformed with said nucleic acid construct and exhibiting at least said one trait.

7. The method of claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 99% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 1011.

8. The method of claim 1 , wherein said isolated polynucleotide comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 686 and 302.

9. The method of claim 3 , wherein said polypeptide comprises an amino acid sequence having at least 99% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 1011.

10. The method of claim 6 , wherein said polypeptide comprises an amino acid sequence having at least 99% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 1011.

11. The method of claim 6 , wherein said isolated polynucleotide comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 686 and 302.

12. A method of increasing yield, growth rate, biomass and/or seed yield of a plant, the method comprising:
(a) transforming plants with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 1011, and,
(b) selecting a plant from step (a) which is transformed with said exogenous polynucleotide, overexpressing said polypeptide and exhibiting an increased dry weight, leaf blade area, leaf number, plot coverage, relative growth rate of leaf number, relative growth rate of plot coverage, relative growth rate of rosette diameter, harvest index, seed yield, rosette area, or rosette diameter as compared to a wild type plant of the same species which is grown under the same growth conditions, thereby increasing the yield, growth rate, biomass and/or seed yield of the plant.

13. The method of claim 11 , wherein said selected transformed plant further exhibits a reduced time to flowering and/or a reduced time to inflorescence emergence as compared to a wild type plant of the same species which is grown under the same growth conditions.

14. A method of producing a plant crop comprising:
(a) growing plants of a crop transformed with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 1011,
(b) selecting a crop plant from step (a) which is transformed with said exogenous polynucleotide, overexpressing said polypeptide and exhibiting an increased dry weight, leaf blade area, leaf number, plot coverage, relative growth rate of leaf number, relative growth rate of plot coverage, relative growth rate of rosette diameter, harvest index, rosette area, or rosette diameter as compared to a wild type plant of the same species which is grown under the same growth conditions, and,
(c) breeding selected transformed crop plant of step (b) to produce said plant crop having crop plants transformed with said exogenous polynucleotide and exhibiting said increased dry weight, leaf blade area, leaf number, plot coverage, relative growth rate of leaf number, relative growth rate of plot coverage, relative growth rate of rosette diameter, harvest index, rosette area, or rosette diameter as compared to a wild type plant of the same species which is grown under the same g

Assignees

• Evogene Ltd

Inventors

• Gil Lidor
• Rimon Knopf Ronit
• Matarasso Noa
• Panik David
• Poraty-Gavra Limor
• Katz Ehud
• Karchi Hagai

Classifications

• C12N15/8261 Primary

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N15/8243

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• C07K14/415

  from plants · CPC title