Mutant with enhanced secretion of L-asparaginase and its application
US-2017145401-A1 · May 25, 2017 · US
Asparaginase mutant with efficient expression, activity and stability
US10337018B1 · US · B1
| Field | Value |
|---|---|
| Publication number | US-10337018-B1 |
| Application number | US-201816177480-A |
| Country | US |
| Kind code | B1 |
| Filing date | Nov 1, 2018 |
| Priority date | Nov 1, 2018 |
| Publication date | Jul 2, 2019 |
| Grant date | Jul 2, 2019 |
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- Title
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Abstract
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The present disclosure discloses an asparaginase mutant with efficient expression, activity and stability, belonging to the technical fields of gene engineering and enzyme engineering. The amino acid sequence of the asparaginase mutant is set forth in SEQ ID NO: 99, SEQ ID NO: 100 or SEQ ID NO: 101; meanwhile, compared with the wild type asparaginase mutant, the extracellular enzyme activity of the asparaginase fusion enzyme mutant is increased by up to 2.25 times, the stability is increased by up to 3.56 times, and the specific enzyme activity is increased by up to 1.34 times.
First claim
Opening claim text (preview).
What is claimed is: 1. An asparaginase mutant, wherein the amino acid sequence of the asparaginase mutant is set forth in SEQ ID NO: 99, SEQ ID NO: 100, or SEQ ID NO: 101. 2. A gene encoding the asparaginase mutant of claim 1 . 3. A recombinant plasmid comprising the gene of claim 2 . 4. The recombinant plasmid of claim 3 , wherein the recombinant plasmid is constructed from a pUC, pET or pGEX. 5. The recombinant plasmid of claim 3 , wherein the recombinant plasmid is constructed from a pET. 6. A host cell comprising the gene of claim 2 . 7. A host cell comprising the recombinant plasmid of claim 3 . 8. A method, wherein the method comprises expressing the asparaginase mutant of claim 1 at a predetermined condition.
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Classifications
Asparaginase {(3.5.1.1)} · CPC title
- C12N15/52Primary
Genes encoding for enzymes or proenzymes · CPC title
Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title
Asparaginase (3.5.1.1) · CPC title
Bacteria; Culture media therefor · CPC title
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- What does patent US10337018B1 cover?
- The present disclosure discloses an asparaginase mutant with efficient expression, activity and stability, belonging to the technical fields of gene engineering and enzyme engineering. The amino acid sequence of the asparaginase mutant is set forth in SEQ ID NO: 99, SEQ ID NO: 100 or SEQ ID NO: 101; meanwhile, compared with the wild type asparaginase mutant, the extracellular enzyme activity of…
- Who is the assignee on this patent?
- Univ Jiangnan
- What technology area does this patent fall under?
- Primary CPC classification C12N15/52. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 02 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).