Recombinant filaggrin polypeptides for cell importation

Patent metadata

Field	Value
Publication number	US-10336797-B2
Application number	US-201013511267-A
Country	US
Kind code	B2
Filing date	Nov 22, 2010
Priority date	Nov 23, 2009
Publication date	Jul 2, 2019
Grant date	Jul 2, 2019

Abstract

Disclosed are recombinant polypeptides that include (a) a filaggrin amino acid sequence and (b) a cell importation signal sequence that includes a motif of two to fifteen amino acids, wherein the motif includes at least one arginine residue and at least one methionine residue. Also disclosed are nucleic acids encoding the recombinant polypeptides of the present invention, and compositions that include the recombinant polypeptides and nucleic acids of the present invention. Methods of treating or preventing a skin disease or skin disorder using the compositions of the present invention are also included, as well as kits that include a sealed containing that includes a recombinant polypeptide of the present invention.

First claim

The invention claimed is:

1. A recombinant polypeptide comprising (a) a filaggrin amino acid sequence; and (b) a cell importation signal sequence comprising a motif of two to fifteen amino acids, wherein the motif comprises at least one arginine residue and at least one methionine residue, wherein the polypeptide comprises SEQ ID NO:21 or SEQ ID NO:22.

2. The polypeptide of claim 1 , wherein the polypeptide consists of SEQ ID NO:21 or SEQ ID NO:22.

3. A skin-care composition comprising an effective amount of a polypeptide as set forth in claim 1 .

4. The composition of claim 3 , comprising a lipid component.

5. The composition of claim 4 , wherein the lipid component comprises a phospholipid.

6. The composition of claim 3 , wherein the composition is a solution, an emulsion, a cream, a lotion, a gel, or an ointment.

7. The composition of claim 6 , wherein the emulsion is an oil-in-water emulsion or a water-in-oil emulsion.

8. The composition of claim 3 , wherein the composition comprises from about 0.001% to about 5.0% by weight of polypeptide.

9. A nucleic acid comprising a nucleic acid sequence encoding a polypeptide as set forth in claim 1 .

10. A pharmaceutical composition comprising a polypeptide as set forth in claim 1 .

11. A method of treating a skin disease or skin disorder selected from the group consisting of ichthyosis, eczema and dermatitis comprising applying an effective amount of the composition of claim 10 to affected skin of a subject in need of such treatment.

12. The method of claim 11 , wherein the subject is a human.

13. The method of claim 11 , wherein the skin disease is ichthyosis vulgaris or atopic dermatitis.

14. The method of claim 11 , wherein the composition is topically applied to a skin surface of the subject.

15. The method of claim 11 , wherein the composition further comprises one or more additional agents that can be applied in the treatment of the skin disease or disorder in the subject.

16. The method of claim 11 , wherein the additional agent is a moisturizer, an exfoliating agent, an anti-inflammatory agent, or an antimicrobial agent.

17. A kit comprising a sealed container comprising the composition of claim 10 .

18. The kit of claim 17 , wherein the sealed container is a vial, a bottle, a dispenser, or a package.

19. The kit of claim 17 , further comprising an applicator.

Assignees

• Stout J Timothy
• Appukuttan Binoy
• Mcfarland Trevor
• Res Found Dev

Inventors

• Stout J Timothy
• Appukuttan Binoy
• Mcfarland Trevor

Classifications

• A61P31/00

  Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics · CPC title

• A61P29/00

  Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] · CPC title

• A61K8/64

  Proteins; Peptides; Derivatives or degradation products thereof · CPC title

• A61Q19/04

  for chemically tanning the skin (topical sun or radiation screening or tanning preparations A61Q17/04) · CPC title

• A61Q17/04

  Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations · CPC title