Spectral imaging for measurement of nuclear pathology features in cancer cells prepared for in situ analysis
US-2018291464-A1 · Oct 11, 2018 · US
US10330927B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10330927-B2 |
| Application number | US-201615061904-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 4, 2016 |
| Priority date | Mar 4, 2015 |
| Publication date | Jun 25, 2019 |
| Grant date | Jun 25, 2019 |
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An optical system images an object region with an optical beam path in an image plane of an image acquisition system. The optical system includes a first optical assembly through which the optical beam path passes and a second optical assembly arranged in the optical beam path on the side of the first optical assembly facing away from the object region. The second optical assembly is a system at least partly compensating longitudinal chromatic aberrations of the first optical assembly occurring in the wavelength range 625 nm≤λ≤850 nm of the light.
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What is claimed is: 1. A surgical microscope comprising: an image acquisition system defining an image plane; an optical system defining an optical beam path and being configured for imaging an object region into said image plane via said optical beam path; said optical system including a first optical assembly mounted in said optical beam path so as to permit said optical beam path to pass therethrough; said first optical assembly having an end facing away from said object region; said optical system further including a second optical assembly mounted in said optical beam path at said end of said first optical assembly; said second optical assembly being configured as a compensation system to at least partially compensate for longitudinal chromatic aberrations of said first optical assembly occurring in a wavelength range of light of 625 nm≤λ≤850 nm; said first optical assembly being configured as an achromatic system corrected for the light of a first wavelength and a second wavelength; said first wavelength being preferably λ≈486 nm and said second wavelength being preferably λ≈656 nm; said achromatic system including lenses made exclusively of normal or standard glasses to provide achromatization with said normal or standard glasses having chromatic aberrations for light in the near infrared and infrared which are approximately five times larger than chromatic aberrations in the visible spectral range; the chromatic aberrations in the near infrared and infrared having a sign opposite to the sign of the chromatic aberrations in the visible spectral range; and, said second optical assembly being an apochromat or semi-apochromat corrected for the light of said first wavelength, which is preferably λ≈486 nm, and for the light of said second wavelength, which is preferably λ≈656 nm, as well as the light of a third wavelength which is preferably λ≈588 nm to reduce the chromatic aberration in the near infrared and infrared of the image of the object region supplied to said image acquisition system. 2. The surgical microscope of claim 1 , wherein: said second optical assembly has a first lens of positive refractive power, a second lens of negative refractive power and a third lens of positive refractive power; said first lens being made from a first glass material and said second lens being made from a second glass material and said third lens being made from said first or a third glass material; wherein the differences ΔP dc of partial dispersions of said first glass material or of the third glass material in relation to the partial dispersion of the second glass material and the differences Δv of the Abbe numbers of said first glass material or said third glass material in relation to the Abbe number of said second glass material are related to one another in a ratio |ΔP dc /Δv|, for which |ΔP dc /Δv|≤0.1‰ applies. 3. The surgical microscope of claim 2 , wherein: said differences ΔP dc of partial dispersions of said first glass material or of said third glass material in relation to the partial dispersion of said second glass material and the differences Δv of the Abbe numbers of said first glass material or said third glass material in relation to the Abbe number of said second glass material are related to one another in a ratio of |ΔP dc /Δv|for which |ΔP dc /Δv|≤0.03‰ applies. 4. The surgical microscope of claim 2 , wherein: said first lens of positive refractive power and said second lens of negative refractive power are combined to form a cemented member; and/or, said first lens of said second optical assembly is mounted at the end thereof facing toward said first optical assembly in said optical beam path; and/or, said third lens of said second optical assembly is mounted so as to face toward said image plane of said image acquisition system in said optical beam path. 5. The surgical microscope of claim 2 , wherein said first lens of said second optical assembly and/or said third lens of said second optical assembly are made of a lens material which has anomalous partial dispersion. 6. The surgical microscope of claim 1 , wherein said first optical assembly includes an afocal zoom system. 7. The surgical microscope of claims 1 , wherein said second optical assembly has a positive refractive power. 8. The surgical microscope of claim 1 , wherein said second optical assembly is an objective effecting an imaging of said object region into said image plane of said image acquisition system. 9. The surgical microscope of claim 1 , wherein said second optical assembly has at least one lens displaceable in said optical beam path relative to said image plane of said image acquisition system. 10. The surgical microscope of claim 9 , wherein said second optical assembly is displaceably mounted so as to permit adjustment of the optical wavelength of said optical beam path from said object region into said image plane of said image acquisition system. 11. The surgical microscope of claim 1 , wherein said image acquisition system includes a color camera configured as a three-chip camera; and, a plurality of image sensors arrayed in said image plane of said image acquisition system. 12. The surgical microscope of claim 11 , further comprising an illumination unit defining an illumination beam path for exciting fluorescence of a dye in said object region; and, including a filter system for filtering out light having a wavelength corresponding to the wavelength of the fluorescence light of the fluorescing dye.
Surgical microscopes (counterbalanced structures for surgical microscopes G02B7/001) · CPC title
with corrections for use in multiple wavelength bands, such as infrared and visible light, e.g. FLIR systems · CPC title
for optical correction, e.g. distorsion, aberration · CPC title
all the components being simple · CPC title
adapted for ultraviolet illumination {; Fluorescence microscopes (G02B21/0076 takes precedence)} · CPC title
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