Electrochemical aptasensors with a gelatin B matrix

Patent metadata

Field	Value
Publication number	US-10330677-B2
Application number	US-201715702938-A
Country	US
Kind code	B2
Filing date	Sep 13, 2017
Priority date	Jul 4, 2013
Publication date	Jun 25, 2019
Grant date	Jun 25, 2019

Abstract

This invention provides:—an aptamer-based electrochemical sensor, wherein said aptamer is covalently bonded to or chemisorbed on an electrode, said aptamer forming a complex with a target molecule and is encapsulated by a gelatin B matrix;—a method of manufacturing said aptamer-based electrochemical sensor;—the use of the aptamer-based electrochemical sensor for the electrochemical determination of a concentration of a target molecule; and—a composite electrode combining a polymeric material and electrically conducting particles for selective analyte detection, wherein said electrode is coated with gelatin type B.

First claim

The invention claimed is:

1. A composite electrode combining a polymeric material and electrically conducting particles, wherein said electrode is coated with a type B gelatin.

2. A composite electrode according to claim 1 , wherein the polymeric material is plasticized polyvinyl chloride.

3. A composite electrode according to claim 1 , wherein the electrically conducting particles are graphite powder.

4. A composite electrode according to claim 1 , wherein the polymeric material is plasticized polyvinyl chloride and wherein the electrically conducting particles are graphite powder.

5. A composite electrode according to claim 1 , wherein an aptamer is covalently bonded to, or chemisorbed on, said electrode.

6. A composite electrode according to claim 1 , wherein an aptamer is covalently bonded to, or chemisorbed on, said electrode, wherein the aptamer is selected to form a robust complex with a target molecule, and wherein the target molecule is selected from the group consisting of vitamins, toxins, antibiotics, therapeutic drugs, diagnostic agents, recreational drugs, catecholamines, metabolites, proteins, and cells.

7. A composite electrode according to claim 1 , wherein an aptamer is covalently bonded to, or chemisorbed on, said electrode, wherein the aptamer is selected to form a robust complex with a target molecule, and wherein the target molecule is dopamine and the aptamer is GTC-TCT-GTG-TGC-GCC-AGA-GAA-CAC-TGG-GGC-AGA-TAT-GGG-CCA-GCA-CAG-AAT-GAG-GCC-C (SEQ ID NO:3).

8. A composite electrode according to claim 1 , wherein an aptamer is covalently bonded to, or chemisorbed on, said electrode, wherein the aptamer is selected to form a robust complex with a target molecule, and wherein the target molecule is chloramphenicol and the aptamer is AGC-AGC-ACA-GAG-GTC-AGA-TGA-CTG-AGG-GCA-CGG-ACA-GGA-GGG-CAT-GGA-GAG-ATG-GCG (SEQ ID NO:1) or the aptamer is AGC-AGC-ACA-GAG-GTC-AGA-TGA-CTG-AGG-GCA-CGG-ACA-GGA-GGG-GGA-GAG-ATG-GCG-TGA-GGT-CCT-ATG-CGT-GCT-ACC-GTG-AA (SEQ ID NO:2).

9. A composite electrode according to claim 1 , wherein an aptamer is covalently bonded to, or chemisorbed on, said electrode, wherein the aptamer is selected to form a robust complex with a target molecule, and wherein the target molecule is cocaine or heroin.

10. A composite electrode according to claim 3 , wherein an aptamer is covalently bonded to, or chemisorbed on, said electrode.

11. A composite electrode according to claim 3 , wherein an aptamer is covalently bonded to, or chemisorbed on, said electrode, wherein the aptamer is selected to form a robust complex with a target molecule, and wherein the target molecule is selected from the group consisting of vitamins, toxins, antibiotics, therapeutic drugs, diagnostic agents, recreational drugs, catecholamines, metabolites, proteins and cells.

Assignees

• Univ Antwerpen

Inventors

• Blust Ronny J P
• Dardenne Freddy
• De Wael Karolien
• Nagels Lucien
• Van Camp Guido F E

Classifications

• G01N27/3275

  Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction · CPC title

• G01N33/5436

  with ligand physically entrapped within the solid phase (liposomes G01N33/5432; immunological test elements G01N33/54386) · CPC title

• G01N2333/78

  Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG] · CPC title

• G01N33/5308

  for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites · CPC title

• G01N33/544

  the carrier being organic · CPC title