Biosensors and biosensor systems with Mn2O3 catalyst as well as methods of making and using the same

Patent metadata

Field	Value
Publication number	US-10330627-B2
Application number	US-201414199003-A
Country	US
Kind code	B2
Filing date	Mar 6, 2014
Priority date	Mar 6, 2013
Publication date	Jun 25, 2019
Grant date	Jun 25, 2019

Abstract

Biosensors and biosensor systems are disclosed that have manganese (III) oxide (Mn 2 O 3 )-based electrodes that can attenuate interference of a detection signal resulting from an analyte-relevant reaction caused by undesired reaction of interferents in a sample. Methods are also disclosed for making and using the same.

First claim

The invention claimed is:

1. An implantable sensor for detecting an analyte in a sample, the sensor comprising:
a substrate, wherein the substrate comprises a sensor surface;
a conductive material superimposing at least a part of the sensor surface of the substrate; and
a first electrode material superimposing at least a part of the conductive material to form a working electrode, wherein the first electrode material comprises an enzyme in a range from 0.1 wt.-% to 50 wt.-% and is adapted to perform at least one detection reaction when the analyte is present in the sample, and wherein the first electrode material further comprises a catalyst configured to catalyze a reduction of hydrogen peroxide (H 2 O 2 ), said catalyst comprising Mn 2 O 3 particles,
wherein the implantable sensor is covered by a biocompatible protective layer.

2. The sensor of claim 1 , wherein the working electrode forms at least one working electrode pad.

3. The sensor of claim 2 , wherein the at least one working electrode pad is electrically connected to at least one electrically conductive contact lead.

4. The sensor of claim 1 further comprising at least one further electrode selected from the group consisting of a reference electrode, a counter electrode, and a combined reference/counter electrode.

5. The sensor of claim 1 , wherein the Mn 2 O 3 particles are present in the first electrode material in a range from 5 wt.-% to 50 wt.-%.

6. The sensor of claim 1 , wherein the first electrode material further comprises at least one material selected from the group consisting of a graphite, a binder, and a combination thereof.

7. The sensor of claim 1 , wherein the enzyme is an oxidase.

8. The sensor of claim 7 , wherein the oxidase is a glucose oxidase.

9. The sensor of claim 1 , wherein the first electrode material is adapted such that hydrogen peroxide (H 2 O 2 ) is generated during the at least one detection reaction.

10. The sensor of claim 1 , wherein the conductive material comprises a component selected from the group consisting of gold, platinum, palladium, silver, and a combination thereof.

11. The sensor of claim 1 , wherein the first electrode material further comprises graphite and the Mn 2 O 3 particles are aggregated to the graphite.

12. The sensor of claim 1 , wherein a usable voltage range for the sensor is from 0.025 V to 0.175 V when compared to a reference electrode comprising Ag/AgCl with 3 M KCl.

13. The sensor of claim 12 , wherein the usable voltage range for the sensor is from 0.050 V to 0.150 V when compared to the reference electrode comprising Ag/AgCl with 3 M KCl.

14. The sensor of claim 1 , wherein the sensor delivers electrical currents in a range from −100 nA to 100 nA.

15. A sensor system for detecting an analyte in a sample, the sensor system comprising:
at least one sensor of claim 1 ; and
at least one detector device, wherein the detector device is electrically connectable to the working electrode and to at least one further electrode, wherein the detector device is adapted to measure at least one parameter selected from the group consisting of an electric current between the working electrode and the at least one further electrode, an electric voltage between the working electrode and the at least one further electrode, and a combination thereof.

16. The sensor system of claim 15 , where at least a part of the sensor surface is superimposed by the biocompatible protective layer.

17. A method of detecting an analyte in a sample, the method comprising the steps of:
applying a voltage to the sample in an implantable sensor comprising:
a. a conductive material, and
b. a first electrode material superimposing at least a part of the conductive material to form a working electrode, wherein the first electrode material is adapted to perform at least one detection reaction when the analyte is present in the sample and is adapted so that hydrogen peroxide (H 2 O 2 ) is generated in response to the analyte during the at least one detection reaction, wherein the first electrode material comprises Mn 2 O 3 particles and an enzyme in a range from 0.1 wt.-% to 50 wt.-%, wherein the Mn 2 O 3 particles catalyze a reduction of hydrogen peroxide (H 2 O 2 ), wherein the implantable sensor is covered by a biocompatible protective layer, and wherein the voltage is within a voltage range at which H 2 O 2 in the sample will be reduced at the working electrode; and
detecting the analyte based upon a measured H 2 O 2 -depedent current.

18. The method of claim 17 , wherein the Mn 2 O 3 particles are present in the first electrode material in a range from 5 wt.-% to 50 wt.-%.

19. The method of claim 17 , wherein the voltage range is from 50 mV to 150 mV when compared to a reference electrode comprising Ag/AgCl with 3 M KCl.

20. The method of claim 17 , wherein the first electrode material further comprises at least one material selected from the group consisting of a graphite, a binder, and a combination thereof.

21. The method of claim 20 , wherein the enzyme is an oxidase.

22. The method of claim 21 , wherein the oxidase is a glucose oxidase and the analyte is glucose.

23. A method of making an implantable sensor for detecting an analyte in a sample, the method comprising the steps of:
providing a substrate comprising a sensor surface;
superimposing at least a part of the sensor surface by a conductive material; and
applying a first electrode material at least on a part of the conductive material to form a first electrode, wherein the first electrode material is adapted to perform at least one detection reaction when the analyte is present in the sample, wherein the first electrode material comprises an enzyme in a range from 0.1 wt.-% to 50 wt.-% and a catalyst configured to catalyze a reduction of hydrogen peroxide (H 2 O 2 ), wherein said catalyst comprises Mn 2 O 3 particles wherein the Mn 2 O 3 particles catalyze the reduction of hydrogen peroxide, and wherein the implantable sensor is covered by a biocompatible protective layer.

24. The method of claim 23 , wherein the Mn 2 O 3 particles are present in the first electrode material in a range from 5 wt.-% to 50 wt.-%.

Assignees

• Roche Diabetes Care Inc

Inventors

• Koelker Karl-Heinz
• Staib Arnulf

Classifications

• G01N33/5438

  Electrodes · CPC title

• C12Q1/004

  mediator-assisted · CPC title

• G01N27/3272

  Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52) · CPC title

• A61B5/14865

  invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors · CPC title

• A61B2562/125

  characterised by the manufacture of electrodes · CPC title