Spider silk protein film, and method for producing same

Patent metadata

Field	Value
Publication number	US-10329332-B2
Application number	US-201314437704-A
Country	US
Kind code	B2
Filing date	Dec 17, 2013
Priority date	Dec 26, 2012
Publication date	Jun 25, 2019
Grant date	Jun 25, 2019

Abstract

A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260° C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same.

First claim

The invention claimed is:

1. A spider silk protein film comprising a polypeptide derived from spider silk proteins,
wherein the spider silk protein film contains dimethyl sulfoxide in an amount from 0.05% to 11.2% by mass,
begins decomposition at between 240 to 260° C.,
the film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm,
the film is transparent and colorless in a visible light region,
the dimethyl sulfoxide is a plasticizer, and
when the spider silk protein film is uniaxially-stretched or biaxially-stretched, the film has a maximum stress of 40 MPa to 100 MPa and a displacement at rupture point (strain) of 10 to 50%.

2. The spider silk protein film according to claim 1 , wherein the spider silk protein film has a refractive index ranging from 1.2 to 1.6 at a wavelength of 590 nm.

3. The spider silk protein film according to claim 1 , wherein the spider silk protein film has moisture absorbency and has a mass reduction of 4-8% at 67 to 94° C. in a thermo-gravimetric/differential thermal analyzer (TG-DTA).

4. The spider silk protein film according to claim 1 , wherein the spider silk protein film is colored by addition of a colorant that can dissolve or disperse in dimethyl sulfoxide.

5. The spider silk protein film according to claim 1 , wherein the protein film consists essentially of the polypeptide derived solely from spider silk proteins and the dimethyl sulfoxide.

6. A spider silk protein film, comprising:
a polypeptide derived from spider silk protein; and
a plasticizer,
wherein the plasticizer is dimethyl sulfoxide, the dimethyl sulfoxide is present in an amount from 0.05% to 11.2% by mass, and the spider silk protein film is a stretched film.

7. The spider silk protein film of claim 6 , wherein the film has a maximum stretch ratio in water of at least 1.3.

8. The spider silk protein film of claim 6 , wherein the spider silk protein film is thermally fixed.

9. The spider silk protein film of claim 6 , wherein the dimethyl sulfoxide is present in an amount between 0.05% and 1.2% by mass.

10. The spider silk protein film of claim 6 , wherein:
the film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm, and
the film is transparent and colorless in a visible light region.

Assignees

• Spiber Inc

Inventors

• SEKIYAMA Kaori
• Ishikawa Mizuki
• Murata Shinya

Classifications

• C08J2389/00

  Characterised by the use of proteins; Derivatives thereof · CPC title

• B29C39/02

  for making articles of definite length, i.e. discrete articles · CPC title

• C08L89/00

  Compositions of proteins; Compositions of derivatives thereof (foodstuff preparations A23J3/00) · CPC title

• C07K14/43518 Primary

  from spiders · CPC title

• C08J5/18

  Manufacture of films or sheets · CPC title