Gas spargers and related container systems

Patent metadata

Field	Value
Publication number	US-10328404-B2
Application number	US-201715596952-A
Country	US
Kind code	B2
Filing date	May 16, 2017
Priority date	Apr 22, 2005
Publication date	Jun 25, 2019
Grant date	Jun 25, 2019

Abstract

A container system includes a collapsible bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A sparger is disposed within the chamber of the bag, the sparger bounding a compartment and having an inside edge that encircles an opening passing through the sparger. At least a portion of the sparger is gas permeable. A tubular member is coupled to the sparger and is in communication with the compartment. A shaft passes into the chamber of the bag and through the opening of the sparger. A mixing element is secured to the shaft and is disposed within the chamber of the bag.

First claim

What is claimed is:

1. A sparger comprising:
a base having a top surface and an opposing bottom surface that extend between an inside edge and an outside edge, the inside edge encircling an opening that passes through the base;
a sparging sheet secured to the base so that a compartment is formed between the base and the sparging sheet that encircles the opening passing through the base, at least a portion of the sparging sheet being gas permeable; and
a tubular member coupled with the base or the sparging sheet so as to be in communication with the compartment.

2. The sparger as recited in claim 1 , wherein the base is ring shaped and the sparing sheet is ring shaped.

3. The sparger as recited in claim 1 , wherein the tubular member comprises a port, a stem, or an elongated tube that is either integrally formed with or connected to the base or the sparging sheet.

4. The sparger as recited in claim 1 , wherein the sparging sheet is comprised of a polymeric nonwoven fabric, a solvent cast polymeric film, an open cell foamed polymer sheet, or a perforated polymer sheet.

5. The sparger as recited in claim 1 , wherein the base is comprised of a flexible sheet of polymeric film.

6. The sparger as recited in claim 1 , wherein the sparging sheet is welded directly to the base.

7. The sparger as recited in claim 1 , further comprising a flexible transition member secured to and extending between the sparging sheet and the base, the transition member being comprised of a different material than the sparging sheet or the base.

8. A container system comprising:
a flexible polymeric bag having an interior surface bounding a chamber; and
the sparger as recited in claim 1 at least partially disposed within the chamber of the polymeric bag, the base of the sparger being secured to the polymeric bag.

9. The container system as recited in claim 8 , further comprising:
a shaft passing into the chamber of the bag and through the opening of the base of the sparger; and
a mixing element secured to the shaft and disposed within the chamber of the bag.

10. A container system comprising:
a bag comprised of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid;
a sparger disposed within the chamber of the bag, the sparger bounding a compartment and having an inside edge that encircles an opening passing through the sparger, at least a portion of the sparger being gas permeable; and
a tubular member coupled to the sparger and being in communication with the compartment.

11. The container system as recited in claim 10 , wherein the sparger further comprises:
a ring shaped base secured to the interior surface of the container; and
a ring shaped sparging sheet secured to the base so that the compartment is formed between the base and the sparging sheet, the compartment encircling the opening passing through the sparger, at least a portion of the sparging sheet being gas permeable.

12. The container system as recited in claim 10 , further comprising:
a shaft passing into the chamber of the bag and through the opening of the sparger; and
a mixing element secured to the shaft and disposed within the chamber of the bag.

13. The container as recited in claim 12 , wherein the mixing element comprises a mixing disk having a plurality of openings extending therethrough.

14. The container as recited in claim 12 , wherein the mixing element comprises an impeller.

15. The container system as recited in claim 10 , further comprising a rigid support housing in which the bag is disposed.

16. A method for sparging a liquid with a gas, the method comprising:
dispensing a liquid into a chamber of a container, a sparger being disposed within the chamber of the container and having an opening passing therethrough;
delivering a gas into a compartment of the sparger so that the gas passes out through the sparger and into the liquid in the chamber; and
moving a mixer passing through the opening in the sparger so as to mix the gas and the liquid within the chamber.

17. The method as recited in claim 16 , wherein the step of dispensing the liquid comprises feeding one or more components into the chamber of the container to produce a liquid culture containing growing cells or microorganisms.

18. The method as recited in claim 16 , wherein the step of moving the mixer comprise repeatedly raising and lowering a shaft passing through the opening of the sparger, a mixing disk being attached to the shaft.

19. The method as recited in claim 16 , wherein the step of moving the mixer comprise rotating a shaft passing through the opening of the sparger, an impeller being attached to the shaft.

20. The method as recited in claim 16 , wherein the container comprises a collapsible bag and the method further comprises positioning the bag within a rigid support housing before dispensing the liquid.

Assignees

• Life Technologies Corp

Inventors

• Goodwin Michael E
• Jones Nephi D
• Larsen Jeremy K

Classifications

• C12M27/04

  with introduction of gas through the stirrer or mixing element · CPC title

• C12M37/00

  Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination (C12M23/38 takes precedence; filtration in general and filters per se B01D24/00-B01D41/00; autoclaves B01J3/04; treatment of microorganisms with electrical or wave energy C12N13/00) · CPC title

• B01F2003/04297

  Operations & Transport · mapped topic

• B01F5/04

  Operations & Transport · mapped topic

• B01F3/04829

  Operations & Transport · mapped topic