Thermocouples comprising a polymer for detecting analytes and related methods

Patent metadata

Field	Value
Publication number	US-10324087-B2
Application number	US-201615095653-A
Country	US
Kind code	B2
Filing date	Apr 11, 2016
Priority date	Apr 11, 2016
Publication date	Jun 18, 2019
Grant date	Jun 18, 2019

Abstract

A device for detecting an analyte includes a thermocouple having an assay polymer over a surface of the thermocouple. The assay polymer is formulated to bind to the analyte, and a heat transfer property of the assay polymer varies responsive to an amount of the analyte bound thereto. A method of forming a sensor includes providing an assay polymer over a thermocouple. A method for detecting an analyte includes passing a liquid containing an analyte adjacent a thermocouple having an assay polymer over a surface thereof, binding an analyte to the assay polymer, detecting a temperature of the thermocouple, and calculating a concentration of the analyte in the liquid based at least in part on the heat transfer property of the assay polymer.

First claim

What is claimed is:

1. A device for detecting an analyte, the device comprising:
a thermocouple; and
an assay polymer on a surface of the thermocouple, the assay polymer formulated to bind to the analyte, wherein a heat transfer property of the assay polymer varies responsive to an amount of the analyte bound thereto.

2. The device of claim 1 , further comprising a processor programmed to calculate an amount of the analyte bound to the assay polymer.

3. The device of claim 2 , wherein the processor is programmed to calculate a concentration of the analyte in a liquid in contact with assay polymer based at least in part on the amount of the analyte bound to the assay polymer.

4. The device of claim 3 , wherein the processor is programmed to detect a phase shift between a thermal wave at a heat transfer element and an attenuated thermal wave at the thermocouple.

5. The device of claim 4 , wherein the processor is programmed to calculate the concentration of the analyte in the liquid based at least in part on a difference in amplitude between the thermal wave at the heat transfer element and the attenuated thermal wave at the thermocouple.

6. The device of claim 1 , further comprising a base material on the thermocouple, wherein the assay polymer is secured to a surface of the base material.

7. The device of claim 1 , wherein the assay polymer comprises a molecularly imprinted polymer.

8. The device of claim 1 , wherein the assay polymer comprises a material selected from the group consisting of DNA, RNA, proteins, and portions and analogs thereof.

9. The device of claim 1 , wherein the assay polymer is formulated to bind to the analyte in a liquid when the liquid is in contact with the assay polymer.

10. A method of forming a device for detecting an analyte comprising a thermocouple, the method comprising:
providing an assay polymer on a surface of the thermocouple, the assay polymer formulated to bind to the analyte, wherein the assay polymer is formulated such that a heat transfer property of the assay polymer varies responsive to an amount of the analyte bound to the assay polymer.

11. The method of claim 10 , wherein providing an assay polymer comprises providing a molecularly imprinted polymer on the surface of the thermocouple.

12. The method of claim 10 , further comprising coating the surface of the thermocouple with a base material and wherein providing an assay polymer on the surface of the thermocouple comprises securing the assay polymer to the base material.

13. The method of claim 12 , further comprising heating the base material to a glass transition temperature thereof prior to securing the assay polymer to the base material.

14. The method of claim 13 , further comprising cooling the base material below the glass transition temperature to crystallize the base material and retain at least a portion of the assay polymer thereon.

15. The method of claim 13 , wherein providing the assay polymer comprises rolling the thermocouple in a powder comprising the assay polymer after the base material has reached the glass transition temperature.

16. The method of claim 14 , further comprising washing the thermocouple to remove an unretained portion of the assay polymer from a surface of the base material.

17. A method for detecting an analyte, the method comprising:
passing a liquid containing an analyte adjacent to and in contact with a thermocouple having an assay polymer on a surface of the thermocouple, the assay polymer formulated to bind to the analyte, wherein a heat transfer property of the assay polymer varies responsive to an amount of the analyte bound to the assay polymer;
binding the analyte to the assay polymer;
detecting a temperature of the thermocouple; and
calculating a concentration of the analyte in the liquid based at least in part on the heat transfer property of the assay polymer.

18. The method of claim 17 , further comprising providing a thermal wave comprising a change in a temperature of the liquid.

19. The method of claim 18 , wherein calculating a concentration of the analyte in the liquid comprises determining a difference in amplitude between the thermal wave at an interface between the liquid and the assay polymer and an attenuated thermal wave at the thermocouple.

20. The method of claim 17 , wherein calculating a concentration of the analyte in the liquid comprises calculating a concentration of a biological analyte in the liquid.

Assignees

• Univ Maastricht
• Acad Ziekenhuis Maastricht

Inventors

• Van Grinsven Bart Robert Nicolaas
• Cleij Thomas Jan

Classifications

• G01N25/482

  concerning the temperature responsive elements (measuring temperature or quantity of heat, thermally-sensitive elements G01K; thermoelectric devices H10N10/00, H10N15/00) · CPC title

• G01N2600/00

  Assays involving molecular imprinted polymers/polymers created around a molecular template · CPC title

• G01N33/54373 Primary

  involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings · CPC title