Antimicrobial susceptibility testing device, antimicrobial susceptibility testing kit, and antimicrobial susceptibility testing method

Patent metadata

Field	Value
Publication number	US-10323266-B2
Application number	US-201515557017-A
Country	US
Kind code	B2
Filing date	Mar 17, 2015
Priority date	Mar 17, 2015
Publication date	Jun 18, 2019
Grant date	Jun 18, 2019

Abstract

Provided is a antimicrobial susceptibility testing device, including: an ATP examination culture plate that includes a reaction vessel, a reagent holding parts for holding reagents to be supplied to the reaction vessel, and a culture solution holding part for holding a culture solution to be supplied to the reaction vessel, and has plural layers that can be joined and separated; a gas feeding path for feeding a gas into the ATP examination culture plate; a heater; an optical detection unit; and a determination unit for determining sensitivity of a bacterial strain contained in the culture solution to a drug based on a detection result of the optical detection unit, wherein when the plural layers of the ATP examination culture plate are joined, at least the culture solution holding part and the reaction vessel are in a sealed state while communicating with each other.

First claim

The invention claimed is:

1. A antimicrobial susceptibility testing device, comprising:
an ATP examination culture plate that includes a reaction vessel, a reagent holding part for holding a reagent to be supplied to the reaction vessel, and a culture solution holding part for holding a culture solution to be supplied to the reaction vessel, and has a plurality of layers that can be joined and separated;
a gas feeding path for feeding a gas into the ATP examination culture plate;
a heater for heating the culture solution holding part,
an optical detection unit for detecting luminescence inside the reaction vessel; and
a determination unit for determining sensitivity of a bacterial strain contained in the culture solution to a drug based on the detection result of the optical detection unit, wherein when the plurality of layers of the ATP examination culture plate are joined, at least the culture solution holding part and the reaction vessel are in a sealed state while communicating with each other.

2. The antimicrobial susceptibility testing device according to claim 1 , wherein the ATP examination culture plate includes a first plate including the reagent holding part and the culture solution holding part, a second plate including the reaction vessel, and a joint portion for joining the first plate and the second plate.

3. The antimicrobial susceptibility testing device according to claim 2 , wherein the reagent holding part and the culture solution holding part are each provided with a nozzle on the reaction vessel side thereof, and the second plate is configured so that the nozzle can be inserted into the reaction vessel, and the reaction vessel is provided so as to keep a depth that allows the first plate and the second plate to be joined with the nozzles inserted therein.

4. The antimicrobial susceptibility testing device according to claim 2 , wherein a first gas feeding part for feeding a gas into the culture solution holding part is connected to the first plate.

5. The antimicrobial susceptibility testing device according to claim 2 , wherein the second plate has the gas feeding path that is connected to a second gas feeding part for feeding a gas into the reaction vessel.

6. The antimicrobial susceptibility testing device according to claim 3 , wherein the length of the nozzle provided in the culture solution holding part is larger than the length of the nozzle provided in the reagent holding part.

7. The antimicrobial susceptibility testing device according to claim 1 , wherein a plurality of the reaction vessels are connected to the gas feeding path.

8. A antimicrobial susceptibility testing kit, comprising:
an ATP examination culture plate that includes a reaction vessel, a reagent holding part for holding a reagent to be supplied to the reaction vessel, and a culture solution holding part for holding a culture solution to be supplied to the reaction vessel, and has a plurality of layers that can be joined and separated; and
a gas feeding path for feeding a gas into the ATP examination culture plate;

wherein when the plurality of layers of the ATP examination culture plate are joined, at least the culture solution holding part and the reaction vessel are in a sealed state while communicating with each other.

9. A antimicrobial susceptibility testing method, comprising:
providing an ATP examination culture plate that includes a reaction vessel, a reagent holding part that holds a reagent to be supplied to the reaction vessel, and a culture solution holding part for holding a culture solution to be supplied to the reaction vessel, and has a plurality of layers, and bringing at least the culture solution holding part and the reaction vessel of the ATP examination culture plate into a sealed state in a manner that the culture solution holding part and the reaction vessel communicate with each other;
supplying a gas from a gas feeding part into the ATP examination culture plate in which the plurality of layers are joined;
heating the culture solution holding part in the sealed state by a heater to culture a bacterial suspension obtained by mixing a bacterium and a drug;
supplying a culture solution obtained by culturing the bacterial suspension into the reaction vessel in the sealed state, while supplying the reagent from the reagent holding part into the reaction vessel;
detecting luminescence inside the reaction vessel that holds a reaction solution of the culture solution and the reagent by an optical detection unit; and
determining sensitivity of the bacterial strain contained in the culture solution to the drug by a determination unit based on the detection result of the optical detection unit.

10. The antimicrobial susceptibility testing method according to claim 9 , wherein
in the ATP examination culture plate, a first plate including the reagent holding part and the culture solution holding part and a second plate including the reaction vessel are provided so that the first plate and the second plate can be joined and separated, and
the first plate and the second plate are joined via a joint portion before the bacterial suspension is cultured, and at least the culture solution holding part and the reaction vessel are brought into a sealed state while communicating with each other.

11. The antimicrobial susceptibility testing method according to claim 10 , wherein the first plate and the second plate are joined with the nozzles provided in the reagent holding part and the culture solution holding part inserted in the reaction vessel.

12. The antimicrobial susceptibility testing method according to claim 11 , wherein
with the first plate and the second plate joined with each other, the culture solution is supplied from the nozzle provided in the culture solution holding part into the reaction vessel, while an ATP extraction reagent is supplied from the nozzle provided in the reagent holding part into the reaction vessel, and the culture solution and the ATP extraction reagent are mixed in the reaction vessel to extract ATP from the culture solution,
subsequently a luminescence reagent that emits light by being mixed with ATP is supplied from the nozzle provided in the reagent holding part into the reaction vessel, and a culture solution held in the reaction vessel after the ATP extraction and the luminescence reagent are mixed, and
the increase or decrease of the amount of luminescence in the reaction vessel that holds the luminescence reagent and the culture solution after the ATP extraction is detected by the optical detection unit.

13. The antimicrobial susceptibility testing method according to claim 12 , wherein a gas is fed from the gas feeding part into at least the culture solution holding part and the reaction vessel of the ATP culture plate in which the first plate and the second plate are joined, and, with the gas atmosphere in the culture solution holding part and the reaction vessel controlled, the bacterial suspension is cultured in the culture solution holding part, and continuously, ATP in the culture solution of the bacterial suspension is extracted in the reaction vessel.

14. The antimicrobial susceptibility testing method according to claim 10 , wherein, with the first plate and the second plate separated, the bacterial suspension held outside the ATP examination culture plate is supplied from the nozzle provided in the culture solution holding part into the culture solution holding part.

15. The antimicrobial susceptibility testing method according to claim 10 , wherein, with the first plate and the second plate joined, the bacterial suspension held outside the ATP ex

Assignees

• Hitachi Ltd

Inventors

• Noda Hideyuki
• Tada Hiroko
• Uematsu Chihiro
• Okanojo Masahiro

Classifications

• C12Q1/66

  involving luciferase · CPC title

• C12M1/34

  Measuring or testing with condition measuring or sensing means, e.g. colony counters · CPC title

• C12Q1/18 Primary

  Testing for antimicrobial activity of a material · CPC title

• C12M45/06

  by chemical means or hydrolysis · CPC title

• G01N21/763

  Bioluminescence · CPC title