Formulation and process for biohydrogen production

Patent metadata

Field	Value
Publication number	US-10323259-B2
Application number	US-201715426815-A
Country	US
Kind code	B2
Filing date	Feb 7, 2017
Priority date	Feb 8, 2016
Publication date	Jun 18, 2019
Grant date	Jun 18, 2019

Abstract

The present invention relates to a process for enhancing hydrogen production in an anaerobic fermentative hydrogen production process. The present invention also provides a production media, more specifically, a sugar production media for use in an anaerobic fermentative hydrogen production process to enhance hydrogen production. The present invention also provides a novel strain Clostridium sp., MTCC 25082 for use in an anaerobic fermentative hydrogen production process for enhancing hydrogen production.

First claim

The invention claimed is:

1. A process for enhancing hydrogen production in an anaerobic fermentative hydrogen production process, the process comprising:
providing a production medium,
incubating the production media with an anaerobic Clostridium sp. at 37° C. and pH 8.5 for anaerobic fermentative reaction to produce hydrogen,
wherein the process comprises supplementing the production medium with lactic acid, acetic acid and butyric acid,
wherein the ratio of acetic acid and butyric acid before the incubating step is 4:1.

2. The process of claim 1 , wherein the production media is a sugar based production media.

3. The process of claim 2 , wherein the sugar is selected from glucose, lactose, xylose, wheat straw pre-hydrolysate (WSPH) and wheat straw enzymatic-hydrolysate (WSEH).

4. The process of claim 1 , wherein the Clostridium sp. is Clostridium sp., MTCC 25082.

5. The process of claim 1 , wherein the amount of lactic acid in the production medium is about 0.3% w/v, the amount of acetic acid is about 2% w/v, and the amount of butyric acid is about 0.5% w/v.

6. The process of claim 1 , wherein acetic acid and butyric acid fractions are recovered from the production medium after the incubating step and are recycled.

7. The process of claim 1 , wherein the amount of hydrogen production is 3.5 to 4.5 mol/mol-sugar.

Assignees

• Indian Oil Corp Ltd
• Dept Of Biotechnology India

Inventors

• PATEL Anil Kumar
• Mathur Anshu Shankar
• Gupta Ravi Prakash
• Tuli Deepak Kumar

Classifications

• C12P3/00 Primary

  Preparation of elements or inorganic compounds except carbon dioxide {(recovery of carbon dioxides as by-products C12F3/02)} · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12R1/145

  Chemistry & Metallurgy · mapped topic

• C12R2001/145

  Clostridium · CPC title

• C12N1/205

  Bacterial isolates · CPC title