Polypeptides having cellobiohydrolase activitiy and polynucleotides encoding same
US-9212354-B2 · Dec 15, 2015 · US
Promoter exhibiting high expression activity in Mortierella microorganisms
US10323250B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10323250-B2 |
| Application number | US-201816135859-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 19, 2018 |
| Priority date | Mar 27, 2013 |
| Publication date | Jun 18, 2019 |
| Grant date | Jun 18, 2019 |
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Abstract
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The present invention aims to provide a promoter showing high expression activity in microorganisms belonging to the genus Mortierella. The present invention provides a polynucleotide which contains any one nucleotide sequence selected from the group consisting of SEQ ID NO: 1 to 28, or a variant thereof.
First claim
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We claim: 1. A vector comprising a polynucleotide selected from: (a) a polynucleotide which contains any one nucleotide sequence selected from SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20 and SEQ ID NO: 21; or (b) a polynucleotide which has a nucleotide sequence sharing an identity of 90% or more with any one nucleotide sequence selected from SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20 and SEQ ID NO: 21 and which shows promoter activity in cells of microorganisms belonging to the genus Mortierella. 2. The vector according to claim 1 , wherein the promoter activity is confirmed as β-D-glucuronidase (GUS) protein activity of at least 500 nmol/(mg·min) upon expression of GUS reporter gene in cells of microorganisms belonging to the genus Mortierella. 3. The vector according to claim 1 , which comprises any one nucleotide sequence selected from SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20 and SEQ ID NO: 21. 4. The vector according to claim 1 , which is DNA. 5. A microbial transformant transformed with the vector according to claim 1 . 6. The transformant according to claim 5 , wherein the transformant is a lipid-producing fungus. 7. The transformant according to claim 6 , wherein the lipid-producing fungus is Mortierella alpina.
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- What does patent US10323250B2 cover?
- The present invention aims to provide a promoter showing high expression activity in microorganisms belonging to the genus Mortierella. The present invention provides a polynucleotide which contains any one nucleotide sequence selected from the group consisting of SEQ ID NO: 1 to 28, or a variant thereof.
- Who is the assignee on this patent?
- Suntory Holdings Ltd
- What technology area does this patent fall under?
- Primary CPC classification C07K14/37. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 18 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).