Cleaning agent containing amylases

Patent metadata

Field	Value
Publication number	US-10323216-B2
Application number	US-201515322930-A
Country	US
Kind code	B2
Filing date	Jun 18, 2015
Priority date	Jun 30, 2014
Publication date	Jun 18, 2019
Grant date	Jun 18, 2019

Abstract

A cleaning agent for hard surfaces and methods for cleaning are provided herein. In one embodiment, the cleaning agent includes at least one first amylase, wherein the first amylase is an α-amylase from Bacillus sp. No. 707 or a functional fragment or a variant thereof. The cleaning agent further includes at least one second amylase, wherein the second amylase is an AA560 α-amylase from Bacillus sp. or a functional fragment or a variant thereof. In another embodiment, the method includes dispensing the cleaning agent into the interior of an automatic dishwasher while a dishwashing program is being executed, before the main washing cycle begins, or in the course of the main washing cycle.

First claim

What is claimed is:

1. A cleaning agent for hard surfaces, comprising:
at least one first amylase including an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 1 over the total length thereof, wherein the first amylase is an α-amylase from Bacillus sp. No. 707 or a functional fragment or a variant thereof; and
at least one second amylase including an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 2 over the total length thereof, wherein the second amylase is an AA560 α-amylase from Bacillus sp. or a functional fragment or a variant thereof,
wherein the first amylase and the second amylase are present in a weight ratio of 10:1, respectively.

2. The cleaning agent according to claim 1 , wherein the first amylase has at least one amino acid substitution at one of positions 172, 202, 208, 255 and 261 in the count according to SEQ ID NO. 1.

3. The cleaning agent according to claim 1 wherein the second amylase has at least one amino acid substitution at one of positions 9, 26, 30, 33, 82, 37, 106, 118, 128, 133, 149, 150, 160, 178, 182, 186, 193, 195, 202, 203, 214, 231, 256, 257, 258, 269, 270, 272, 283, 295, 296, 298, 299, 303, 304, 305, 311, 314, 315, 318, 319, 320, 323, 339, 345, 361, 378, 383, 419, 421, 437, 441, 444, 445, 446, 447, 450, 458, 461, 471, 482 and 484 and/or one of the deletions D183* and G184* in the count according to SEQ ID NO. 2.

4. The cleaning agent according to claim 3 , wherein the second amylase, in the count according to SEQ ID NO. 2, includes amino acid substitutions at three or more of positions 9, 26, 149, 182, 186, 202, 257, 295, 299, 323, 339 and 345.

5. The cleaning agent according to claim 3 , wherein the second amylase includes the following amino acid substitutions and/or deletions in the count according to SEQ ID NO. 2:
(i) M9L+M323T;
(ii) M9L+M202L/T/V/I+M323T;
(iii) M9L+N195F+M202L/T/V/I+M323T;
(iv) M9L+R118K+D183*+G184*+R320K+M323T+R458K;
(v) M9L+R118K+D183*+G184*+M202L/T/V/I+R320K+M323T+R458K;
(vi) M9L+G149A+G182T+G186A+M202L+T257I+Y295F+N299Y+M323T+A339S+E345R;
(vii) M9L+G149A+G182T+G186A+M202I+T257I+Y295F+N299Y+M323T+A339S+E345R;
(viii) M9L+R118K+G149A+G182T+D183*+G184*+G186A+M202L+T257I+Y295F+N299Y+R320K+M323T+A339S+E345R+R458K;
(ix) M9L+R118K+G149A+G182T+D183*+G184*+G186A+N195F+M202L+T257I+Y295F+N299Y+R320K+M323T+A339S+E345R+R458K;
(x) M9L+R118K+G149A+G182T+D183*+G184*+G186A+M202I+T257I+Y295F+N299Y+R320K+M323T+A339S+E345R+R458K;
(xi) M9L+R118K+D183*+D184*+N195F+M202L+R320K+M323T+R458K;
(xii) M9L+R118K+D183*+D184*+N195F+M202T+R320K+M323T+R458K;
(xiii) M9L+R118K+D183*+D184*+N195F+M202I+R320K+M323T+R458K;
(xiv) M9L+R118K+D183*+D184*+N195F+M202V+R320K+M323T+R458K;
(xv) M9L+R118K+N150H+D183*+D184*+N195F+M202L+V214T+R320K+M323T+R458K; or
(xvi) M9L+R118K+D183*+D184*+N195F+M202L+V214T+R320K+M323T+E345N+R458K.

6. The cleaning agent according to claim 1 , wherein the cleaning agent furthermore comprises at least one protease.

7. The cleaning agent according to claim 6 , wherein the at least one protease:
(a) comprises a subtilisin 309 from Bacillus lentus or a functional fragment or a variant thereof, comprises an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 3 over the total length thereof, and has at least one amino acid substitution at one of positions 9, 15, 66, 212 and 239 in the count according to SEQ ID NO. 3; and/or
(b) comprises a subtilisin 309 from Bacillus lentus or a functional fragment or a variant thereof, comprises an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 3 over the total length thereof, and has an amino acid substitution at position 99 and an insertion of one amino acid between the amino acids at positions 99 and 100 in the count according to SEQ ID NO. 3; and/or
(c) comprises an alkaline protease from Bacillus lentus DSM 5483 or a functional fragment or a variant thereof, includes an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 4 over the total length thereof; and/or
(d) comprises an amino acid sequence according to one of SEQ ID NOs: 5 to 8.

8. The cleaning agent according to claim 7 , wherein the at least one protease comprises two or more proteases.

9. The cleaning agent according to claim 1 , wherein the weight fraction of each of the amylases, based on the corresponding active protein, in the total weight of the agent is from about 1×10 −8 to about 5 wt. %.

10. The cleaning agent according to claim 1 , comprising at least one further component selected from the group consisting of builders, surfactants, polymers, bleaching agents, bleach catalysts, bleach activators, non-protease and non-amylase enzymes, corrosion inhibitors, glass corrosion inhibitors, disintegrants, odorants and perfume carriers.

11. The cleaning agent according to claim 1 , wherein the cleaning agent is an automatic dishwashing agent, and the
(1) automatic dishwashing agent is present in solid form; and/or
(2) the automatic dishwashing agent is present in preportioned form; and/or
(3) the automatic dishwashing agent comprises multiple compositions that are physically separated from each other.

12. Method for cleaning dishes in an automatic dishwasher, wherein a cleaning agent according to claim 1 is dispensed into the interior of an automatic dishwasher while a dishwashing program is being executed, before the main washing cycle begins, or in the course of the main washing cycle.

13. The cleaning agent according to claim 2 , wherein the first amylase has at least one amino acid substitution selected from the group consisting of M202L, S255N and R172Q.

14. The cleaning agent according to claim 4 , wherein the second amylase, in the count according to SEQ ID NO. 2, includes amino acid substitutions and/or deletions at positions 118, 183, 184, 195, 320 and 458.

15. The cleaning agent according to claim 14 , wherein the second amylase, in the count according to SEQ ID NO. 2, has one or more of the following substitutions and/or deletions: R118K, D183*, G184*, N195F, R320K and/or R458K.

16. A cleaning agent for hard surfaces, comprising:
at least one first amylase including an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 1 over the total length thereof, wherein the first amylase is an α-amylase from Bacillus sp. No. 707 or a functional fragment or a variant thereof;
at least one second amylase including an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 2 over the total length thereof, wherein the second amylase is an AA560 α-amylase from Bacillus sp. or a functional fragment or a variant thereof,
a first protease comprising a subtilisin 309 from Bacillus lentus or a functional fragment or a variant thereof, comprising an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 5 over the total length thereof; and
a second protease comprising a subtilisin 309 from Bacillus lentus or a functional fragment or a variant thereof, comprising an amino acid sequence that is at least 80% identical to the amino acid sequence indicated in SEQ ID NO. 6 over the total length thereof,
wherein the first amylase and the second amylase are present in a weight ratio of 10:1, respectively.

17. The cleaning agent according to claim 16 , wherein the first amylase has an amino acid substitut

Assignees

• Henkel Ag & Co Kgaa

Inventors

• Vockenroth Inga Kerstin
• Weber Thomas
• Menke Silke
• Eiting Thomas
• Mussmann Nina
• Bastigkeit Thorsten
• Wrubbel Noelle

Classifications

• C12N9/2417 Primary

  from microbiological source · CPC title

• C11D3/386 Primary

  Preparations containing enzymes {, e.g. protease or amylase} · CPC title

• C12Y302/01001

  Alpha-amylase (3.2.1.1) · CPC title

• C11D11/0023

  Chemistry & Metallurgy · mapped topic

• C11D3/38609 Primary

  Protease or amylase in solid compositions only · CPC title