High oleic acid oils

Patent metadata

Field	Value
Publication number	US-10323209-B2
Application number	US-201313869763-A
Country	US
Kind code	B2
Filing date	Apr 24, 2013
Priority date	Apr 25, 2012
Publication date	Jun 18, 2019
Grant date	Jun 18, 2019

Abstract

The present invention relates to extracted lipid with high levels, for example 90% to 95% by weight, oleic acid. The present invention also provides genetically modified plants, particularly oilseeds such as safflower, which can used to produce the lipid. Furthermore, provided are methods for genotyping and selecting plants which can be used to produce the lipid.

First claim

The invention claimed is:

1. Lipid extracted from safflower seed, the lipid comprising triacylglycerols (TAG) which consist of fatty acids esterified to glycerol, wherein
i) the fatty acids comprise palmitic acid and oleic acid,
ii) at least 95% by weight of the lipid is TAG,
iii) 90% to 95% by weight of the total fatty acid content of the lipid is oleic acid,
iv) less than 3%, by weight of the total fatty acid content of the lipid is palmitic acid,
v) the lipid has an oleic desaturation proportion (ODP) of less than 0.037 and/or a palmitic-linoleic-oleic value (PLO) of less than 0.063, and
vi) less than 2.25% by weight of the total fatty acid content of the lipid is linoleic acid.

2. The lipid of claim 1 which has one or more or all of the following features,
a) 90% to 94% by weight of the total fatty acid content of the lipid is oleic acid,
b) 0.1% to 3% by weight of the total fatty acid content of the lipid is polyunsaturated fatty acids (PUFA),
c) less than 1% by weight of the total fatty acid content of the lipid is a-linolenic acid (ALA),
d) 0.5% to 1% by weight of the total fatty acid content of the lipid is 18:1Δ11,
e) 90% to 96% by weight of the total fatty acid content of the lipid is monounsaturated fatty acids,
f) the lipid has an oleic monounsaturation proportion (OMP) of less than 0.02,
g) the lipid is in the form of a purified oil, and
h) the lipid is non-hydrogenated.

3. The lipid of claim 1 , wherein less than 0. 5%, by weight of the total fatty acid content of the lipid, is α-linolenic acid.

4. The lipid of claim 1 , wherein 55% to 80% of the TAG content of the lipid is triolein.

5. The lipid of claim 1 , wherein less than 5% of the oleic acid content of the lipid is in the form of diacylglycerols (DAG).

6. The lipid of claim 1 , which is in the form of an oil, wherein at least 90% by weight of the oil is the lipid.

7. The lipid of claim 1 which further comprises one or more sterols.

8. The lipid of claim 7 which is in the form of an oil, and which comprises less than 5 mg of sterols/g of oil.

9. The lipid of claim 8 which comprises one or more or all of
a) 1.5% to 4.5% of the total sterol content is ergost-7-en-3β-ol,
b) 0.5% to 3% of the total sterol content is triterpenoid alcohol,
c) 8.9% to 20% of the total sterol content is Δ7-stigmasterol/stigmast-7-en-3β3-ol, and
d) 1.7% to6.1% of the total sterol content is Δ7-avenasterol.

10. The lipid of claim 1 which has a volume of at least 1 litre and/or a weight of at least 1 kg, and/or which was extracted from seeds obtained from field-grown safflower plants.

11. A composition comprising a first component which is lipid of claim 1 , and a second component, where the composition was produced by mixing the lipid with the second component.

12. The composition of claim 11 , wherein the second component is a non-lipid substance.

13. A process for producing oil, the process comprising
i) obtaining safflower seed whose oil content is a lipid as defined in claim 1 , and
ii) extracting oil from the safflower seed so as to thereby produce the oil.

14. A process for producing an industrial product, the process comprising the steps of:
i) obtaining the lipid of claim 1 ,
ii) optionally physically processing the lipid,
ii) converting at least some of the lipid, or the physically processed product of step ii), to the industrial product by applying heat, chemical, or enzymatic means, or any combination thereof, to the lipid, and
iii) recovering the industrial product,
thereby producing the industrial product.

15. A method of producing a feedstuff, the method comprising admixing the lipid of claim 1 with at least one other food ingredient.

16. The lipid of claim 1 , wherein less than 2. 75%, by weight of the total fatty acid content of the lipid, is palmitic acid.

17. The lipid of claim 1 , wherein the ODP of the fatty acid content of the lipid is 0.033 to 0.01.

18. The lipid of claim 1 , wherein the PLO value of the fatty acid content of the lipid is 0.020 to 0.063.

19. The lipid of claim 18 , wherein the PLO value of the fatty acid content of the lipid is 0.020 to 0.055.

20. Lipid extracted from safflower seed, the lipid comprising triacylglycerols (TAG) which consist of fatty acids esterified to glycerol, wherein
i) the fatty acids comprise palmitic acid and oleic acid,
ii) at least 95% by weight of the lipid is TAG,
iii) 91% to 95% by weight of the total fatty acid content of the lipid is oleic acid,
iv) less than 3%, by weight of the total fatty acid content of the lipid is palmitic acid,
v) the lipid has an oleic desaturation proportion (ODP) of less than 0.037 and/or a palmitic-linoleic-oleic value (PLO) of less than 0.063, and
vi) less than 2.25% by weight of the total fatty acid content of the lipid is linoleic acid.

Assignees

• Wood Craig Christopher
• Liu Qing
• ZHOU Xue Rong
• Green Allan
• Singh Surinder Pal
• Cao Shijiang
• Commw Scient Ind Res Org

Inventors

• Wood Craig Christopher
• Liu Qing
• Zhou Xue-Rong
• Green Allan
• Singh Surinder Pal
• Cao Shijiang

Classifications

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• A23D9/00 Primary

  Other edible oils or fats, e.g. shortenings or cooking oils (preservation of other edible oils A23B20/30) · CPC title

• C12N9/0004

  Oxidoreductases (1.) · CPC title

• C11B1/10 Primary

  by extracting · CPC title