Laser ablation cell

Patent metadata

Field	Value
Publication number	US-10319576-B2
Application number	US-201615336361-A
Country	US
Kind code	B2
Filing date	Oct 27, 2016
Priority date	Mar 22, 2013
Publication date	Jun 11, 2019
Grant date	Jun 11, 2019

Abstract

A laser ablation cell ( 1 ) comprises a flow channel ( 11 ) having an essentially constant cross-sectional area so as to ensure a strictly laminar flow in the flow channel. A sample chamber ( 21 ) is provided adjacent to a lateral opening ( 14 ) of the flow channel. A laser beam ( 41 ) enters the sample chamber ( 21 ) through a lateral window ( 16 ) and impinges on a surface ( 24 ) of a sample ( 23 ) to ablate material from the sample. The sample may be positioned in such a distance from the flow channel that the laser-generated aerosol mass distribution has its center within the flow channel. This leads to short aerosol washout times. The laser ablation cell is particularly well suited for aerosol generation in inductively coupled plasma mass spectrometry (ICPMS), including imaging applications.

First claim

What is claimed is:

1. A laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) system for imaging of biological samples, comprising:
a laser ablation cell comprising a flow channel and a sample chamber;
wherein the flow channel extends from a carrier gas inlet to a mixed gas outlet;
wherein the flow channel comprises a lateral opening and a lateral window;
wherein the sample chamber comprises a sheath gas inlet;
a laser adapted to shoot a laser beam through both the lateral window and the lateral opening of the ablation cell and impinge on the surface of a sample received by the sample chamber to generate a plume from the sample;
an inductively coupled plasma (ICP) torch coupled to an outlet of the ablation cell for receiving and ionizing the plume; and
a mass analyzer coupled to the ICP torch.

2. The system of claim 1 , wherein the carrier gas inlet is outside the sample chamber.

3. The system of claim 1 , wherein carrier gas flowing through the carrier gas inlet does not pass through the sample chamber.

4. The system of claim 1 , wherein the system is arranged to deliver Argon into the carrier gas inlet and Helium into the sheath gas inlet.

5. The system of claim 3 , wherein the system is arranged to deliver Argon into the carrier gas inlet and Helium into the sheath gas inlet.

6. The system of claim 1 , wherein the lateral opening but not the lateral window is adjacent to the sample chamber.

7. The system of claim 1 , wherein the laser ablation cell is adapted to have a washout time of 100 ms or less.

8. The system of claim 1 , wherein the flow channel is in a cell top of the laser ablation cell.

9. The system of claim 8 , wherein the cell top is detachable from the sample chamber.

10. The system of claim 1 , wherein the system further comprises a positioning device operable to change the relative position between a sample received by the sample chamber and the laser beam.

11. The system of claim 10 , wherein the positioning device is operable to scan the laser beam over the sample surface.

12. The system of claim 1 , wherein the lateral opening has a cross sectional area that is not more than 20 mm 2 .

13. The system of claim 1 , wherein the laser is a UV laser.

14. The system of claim 1 , wherein the laser is operable to shoot a pulsed laser beam, and wherein each laser pulse will generate the plume from the sample in the sample chamber.

15. The system of claim 1 , wherein the laser has a laser spot size of 4 μm or less.

16. The system of claim 1 , wherein the mass analyzer is a time-of-flight mass analyzer.

17. The system of claim 1 , wherein the sample chamber comprises a biological sample comprising a plurality of different target molecules each labeled with an antibody attached to a lanthanide labeling atom.

18. The system of claim 17 , wherein the biological sample comprises at least 10 different labeling atoms.

19. A laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) system for imaging of biological samples, comprising:
a laser ablation cell comprising a flow channel and a sample chamber;
wherein the flow channel extends from a carrier gas inlet to a mixed gas outlet;
wherein the carrier gas inlet is outside the sample chamber;
wherein the flow channel comprises a lateral opening and a lateral window;
wherein the sample chamber comprises a sheath gas inlet;
a laser adapted to shoot a laser beam through both the lateral window and the lateral opening of the ablation cell and impinge on the surface of a sample received by the sample chamber to generate a plume from the sample;
an inductively coupled plasma (ICP) torch coupled to an outlet of the ablation cell for receiving and ionizing the plume; and
a mass analyzer coupled to the ICP torch.

20. A laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) system for imaging of biological samples, comprising:
a laser ablation cell comprising a flow channel and a sample chamber;
wherein the flow channel extends from a carrier gas inlet to a mixed gas outlet;
wherein the flow channel comprises a lateral opening and a lateral window;
wherein the sample chamber comprises a sheath gas inlet;
wherein carrier gas flowing through the carrier gas inlet does not pass through the sample chamber;
a laser adapted to shoot a laser beam through both the lateral window and the lateral opening of the ablation cell and impinge on the surface of a sample received by the sample chamber to generate a plume from the sample;
an inductively coupled plasma (ICP) torch coupled to an outlet of the ablation cell for receiving and ionizing the sample; and
a mass analyzer coupled to the ICP torch.

Assignees

• Eth Zuerich
• Scherrer Inst Paul

Inventors

• Günther Detlef
• Grolimund Daniel
• Wang Hao

Classifications

• G01N33/57515

  of the breast · CPC title

• G01N33/60

  involving radioactive labelled substances · CPC title

• H01J49/0463

  Desorption by laser or particle beam, followed by ionisation as a separate step (sample holder per se H01J49/0418) · CPC title

• H01J49/40

  Time-of-flight spectrometers (H01J49/36 takes precedence) · CPC title

• H01J49/105 Primary

  using high-frequency excitation, e.g. microwave excitation, Inductively Coupled Plasma [ICP] · CPC title