Process for the hydrolysis of lignocellulosic material, wherein the hydrolysate is used for microbial hydrolase production

Patent metadata

Field	Value
Publication number	US-10316341-B2
Application number	US-201515312206-A
Country	US
Kind code	B2
Filing date	May 20, 2015
Priority date	May 21, 2014
Publication date	Jun 11, 2019
Grant date	Jun 11, 2019

Abstract

The present invention is directed to a process for self-sufficient hydrolysis of lignocellulosic material. In an additional aspect, the present invention is directed to a process for the production of an organic product and the organic product produced according to this process.

First claim

What is claimed is:

1. A process for self-sufficient enzymatic hydrolysis of lignocellulose-containing material, comprising the steps
(a) subjecting a lignocellulose-containing material to a pretreatment in a pretreatment device;
(b) contacting the pretreated lignocellulose-containing material of step a) with at least one hydrolase comprising one or more activities selected from the activities of exo- or endocellulases, exo- or endohemicellulases, and esterases in a hydrolysis vessel to form a hydrolyzate;
(c) separating the hydrolyzate and subsequently dividing the hydrolyzate into two parts (i) and (ii), wherein part (i) is directed to a cultivation vessel;
(d) fermenting part (i) of the hydrolyzate with at least one microorganism and/or fungus capable of the production of said hydrolase; and
(e) redirecting the fermented hydrolyzate of step d) to the hydrolysis vessel of step b);
wherein the hydrolyzate is treated to remove at least one substance inhibitory to at least one enzyme and/or at least one microorganism and/or fungus.

2. The process according to claim 1 , wherein the at least one substance inhibitory to at least one enzyme and/or at least one microorganism and/or fungus is a substance inhibitory to hydrolase and/or inhibitory to at least one microorganism and/or fungus capable of the production of hydrolase.

3. The process according to claim 1 , wherein the substance is inhibitory to at least one fungus and/or microorganism selected from the group consisting of Trichoderma sp. (anamorph: Hypocrea . sp), Saccharomyces sp., Clostridium sp., Lactobacillus sp., Pichia sp. and Talaromyces sp.

4. The process according to claim 1 , wherein the substance is removed from the pretreated lignocellulose-containing material according to step (a) and/or from part (i) and/or (ii) of the hydrolyzate according to step (c).

5. The process according to claim 1 , wherein the substance is removed by adding at least one adsorbent to the pretreated lignocellulose-containing material and/or part (i) and/or (ii) of the hydrolyzate according to step (c).

6. The process according to claim 5 , wherein the adsorbent is selected from the group consisting of activated carbon, silica, silicate minerals, zeolites, charcoal, clay, ion exchange resins and mixtures thereof.

7. The process according to claim 1 , wherein the substance is removed from part (i) and/or (ii) of the hydrolyzate according to step (c) by evaporation.

8. The process according to claim 1 , wherein an additional amount of from 0.1 to 30% (wt./vol.) of lignocellulose-containing material is added to the cultivation vessel.

9. The process according to claim 8 , wherein the lignocellulose-containing material is obtained by separating it from the pretreatment vessel.

10. The process according to claim 1 , wherein the ratio of part (i) of the hydrolyzate to part (ii) is from 0.01 to 1.

11. The process according to claim 1 , wherein the concentration of soluble sugars during the fermentation according to step d) is below 10% (wt./vol.).

12. The process according to claim 1 , further comprising the step
(f1) contacting part (ii) of the hydrolyzate with at least one microorganism and/or fungus capable of the production of an organic compound selected from the group consisting of organic acids, amino acids, caprolactams, antibiotics, vitamins, enzymes, nucleotides/ nucleosides, biogas, proteins, polysaccharides, amino glucans, organic solvents, biofuels, biosurfactants, aminoglucans, sugar derivatives and mixtures thereof; or
(f2) subjecting part (ii) of the hydrolyzate to a chemical conversion, catalytic conversion, chromatographic separation, membrane separation and/or crystallisation process.

13. The process according to claim 1 , wherein said at least one hydrolase includes an exo- or endocellulase activity selected from the group consisting of cellobiohydrolase (CBH) I, II, endoglucanase (EG) I-IV, and beta-glucosidase (BGL) and also includes an exo- or endohemicellulase activity selected from the group consisting of xylanase, xylosidase, xylobiase, arabinase, arabinofucosidase, mannanase, mannosidase, galactase and galactosidase.

14. The process according to claim 13 , wherein said at least one hydrolase includes all of the following activities: CBH I, CBH II, EG I-IV, BGL, xylanase, xylosidase, xylobiase, arabinase, arabinofucosidase, mannanase, mannosidase, galactase, and galactosidase.

Assignees

• Clariant Int Ltd

Inventors

• Zavrel Michael
• Dennewald Danielle
• Bartuch Joerg
• Marckmann Henning

Classifications

• C12Y302/01021

  Beta-glucosidase (3.2.1.21) · CPC title

• C12Y302/01004

  Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title

• C12P2201/00

  Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis · CPC title

• C12P19/28

  N-glycosides · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title