Photoluminescent nanoparticles and their synthesis and uses

Patent metadata

Field	Value
Publication number	US-10316246-B2
Application number	US-201715610071-A
Country	US
Kind code	B2
Filing date	May 31, 2017
Priority date	May 31, 2016
Publication date	Jun 11, 2019
Grant date	Jun 11, 2019

Abstract

The present invention discloses a method of preparing polymeric photoluminescent dots in water using inexpensive non-conjugated polymers. The resulting polymeric photoluminescent dots display the following properties: excellent water dispersibility, low toxicity, high absorptivity, good photo-stability and high quantum yield. The photoluminescent properties are not influenced by aggregation-caused quenching effect. The method is amenable to scale-up and is environmentally friendly.

First claim

What is claimed is:

1. A photoluminescent nanoparticle obtained by a method comprising the following steps:
(a) mixing an amine-containing polymer with a monomer of an α,β-unsaturated carboxylic acid or α,β-unsaturated carboxylic acid anhydride, wherein said amine-containing polymer and said monomer undergo Michael addition reaction and amidation reaction to form a prepolymer, wherein said monomer has a formula of CH 2 ═CR 1 R 2 , wherein R 1 is hydrogen, alkyl having 1-10 carbon atoms, or phenyl, and R 2 is —COOH or —COOCOR 3 wherein R 3 is alkyl, phenyl, benzyl, or heteroaryl;
(b) adding a radical initiator to the reaction mixture containing said prepolymer and said monomer from step (a) to form a poly(amine-acid) polymer; and
(c) allowing said poly(amine-acid) polymer to self-assemble via electrostatic interaction between carboxyl group and amine group to form said nanoparticle.

2. The nanoparticle of claim 1 , wherein said amine-containing polymer is synthetic or natural polymer.

3. The nanoparticle of claim 1 , wherein said amine-containing polymer is water soluble.

4. The nanoparticle of claim 1 , wherein said amine-containing polymer is selected from the group consisting of polyethyleneimine (PEI), linear Polyethylenimine, poly(allylamine), poly (acrylamide), poly[dimethylsiloxane-co-(3-aminopropyl)methylsiloxane], poly(vinylamine), poly(N-methylvinylamine), chitosan, polylysine, casein, gelatin, bovine serum albumin and protein.

5. The nanoparticle of claim 1 , wherein said amine-containing polymer is polyethyleneimine.

6. The nanoparticle of claim 1 , wherein said monomer is methacrylic acid.

7. The nanoparticle of claim 1 , wherein the nanoparticle has hydrodynamic diameter (D h ) value ranging from 30 nm to 500 nm.

8. The nanoparticle of claim 1 , wherein the nanoparticle has a polydispersity index (PDI) value ranging from 0.05 to 0.2 in water.

9. The nanoparticle of claim 1 , wherein the nanoparticle has surface charges ranging from 20 mV to 55 mV.

10. The nanoparticle of claim 1 , wherein the nanoparticle has IR absorption bands at 3500-2800 cm −1 , 1750-1600 cm −1 , 1560-1540 cm −1 , 1480-1450 cm −1 , 1410-1390 cm −1 and 1200-1100 cm −1 .

11. The nanoparticle of claim 1 , having a carbon to nitrogen average ratio in the range of 2:1 to 45:1 per dry weight.

12. The nanoparticle of claim 1 , wherein 0.1-1.0 mg/ml of said nanoparticle in water has photoluminescence peak ranging from 400 nm to 420 nm under excitation wavelength of 360 nm, or 3-7.5 mg/ml of said nanoparticle in water has photoluminescence peak ranging from 450 nm to 480 nm under excitation wavelength of 420 nm, or 8-20 mg/ml of said nanoparticle in water has photoluminescence peak ranging from 470 nm to 550 nm under excitation wavelength of 420 nm.

13. The nanoparticle of claim 1 , having an absolute quantum yield up to 85%.

14. The nanoparticle of claim 1 , having a fluorescence lifetime ranging from 0.3-10 ns.

15. The nanoparticle of claim 1 , having a phosphorescence lifetime ranging from 1-1000 μs.

16. A method for preparing a photoluminescent nanoparticle, comprising the steps of:
(a) reacting an amine-containing polymer and a monomer of an α,β-unsaturated carboxylic acid or α,β-unsaturated carboxylic acid anhydride to form a prepolymer via Michael addition and amidation; and
(b) adding a radical initiator to the reaction mixture containing prepolymer and monomer from step (a) to form a poly(amine-acid) polymer; and
(c) allowing said poly(amine-acid) polymer to self-assemble via electrostatic interaction between carboxyl group and amine group to form said nanoparticle.

17. The method of claim 16 , wherein the weight ratio of said monomer to said amine-containing polymer ranges from 1:10 to 10:1.

18. The method of claim 16 , wherein said method is performed in a solvent selected from the group consisting of water, HCl, H 2 SO 4 , HNO 3 , acetic acid, trifluoroacetic acid and any mixture thereof.

19. The method of claim 16 , wherein said radical initiator is selected from the group consisting of hydroperoxide, potassium persulfate, 2,2′-azobis(2-amidinopropane) hydrochloride, and water-soluble azo initiators.

Assignees

• Univ Hong Kong Polytechnic

Inventors

• Li Pei
• Yao Yuan
• Niu Dechao

Classifications

• C08F283/002

  on to polymers modified by after-treatment · CPC title

• C08F283/00

  Macromolecular compounds obtained by polymerising monomers on to polymers provided for in subclass C08G · CPC title

• C08F220/06

  Acrylic acid; Methacrylic acid; Metal salts or ammonium salts thereof · CPC title

• C09K2211/14

  Macromolecular compounds · CPC title

• C09K11/06 Primary

  containing organic luminescent materials · CPC title