Concentrated aqueous silk fibroin solution and use thereof

Patent metadata

Field	Value
Publication number	US-10314938-B2
Application number	US-201715434484-A
Country	US
Kind code	B2
Filing date	Feb 16, 2017
Priority date	Apr 10, 2003
Publication date	Jun 11, 2019
Grant date	Jun 11, 2019

Abstract

The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

First claim

What is claimed is:

1. A composition consisting essentially of at least 4% wt silk fibroin protein but not more than 10% wt silk fibroin protein, water, and a therapeutic agent.

2. The composition of claim 1 , wherein said silk fibroin protein is obtained from a solution of dissolved silkworm silk, genetically engineered silk, or dissolved spider silk.

3. The composition of claim 1 , wherein the therapeutic agent is a small molecule, a protein, a peptide, a polysaccharide, a glycoprotein, a lipoprotein, or a nucleic acid.

4. The composition of claim 1 , wherein the therapeutic agent is a small molecule selected from the group consisting of vitamins, sedatives, hypnotics, antibiotics, antiviral agents, steroids, growth factor inhibitors, growth factor promoters, prostaglandins and radiopharmaceuticals.

5. The composition of claim 1 , wherein the therapeutic agent is selected from the group consisting of anti-infectives, chemotherapeutic agents, anti-rejection agents, analgesics and analgesic combinations, anti-inflammatory agents, hormones, anti-angiogenic agents, anti-thrombotics, anti-metabolics, antimitotics, fibrinolytics, and growth factors.

6. The composition of claim 5 , wherein the growth factor is selected from the group consisting of bone morphogenic proteins, bone morphogenic-like proteins, epidermal growth factor, fibroblast growth factor, platelet derived growth factor, insulin-like growth factor, transforming growth factors, and vascular endothelial growth factor.

7. The composition of claim 1 , wherein the therapeutic agent is an antibody.

8. The composition of claim 1 , wherein the therapeutic agent is present as a liquid or a finely divided solid.

9. The composition of claim 1 , wherein the therapeutic agent further comprises a pharmaceutically acceptable carrier.

10. A composition consisting essentially of at least 4% wt silk fibroin protein but not more than 10% wt silk fibroin protein, water, and an additive.

11. The composition of claim 10 , wherein said silk fibroin protein is obtained from a solution of dissolved silkworm silk, genetically engineered silk, or dissolved spider silk.

12. The composition of claim 10 , wherein the additive is a biologically and/or pharmaceutically active compound.

13. The composition of claim 10 , wherein the additive is an antibody, a deoxyribonucleic acid, a ribonucleic acid, a modified ribonucleic acid and protein composite, a glycogen, a sugar, an alcohol, or cytokine.

14. The composition of claim 10 , wherein the additive is a cell attachment mediator, a biologically active ligand, a growth factor, or an osteoinductive substance.

15. The composition of claim 14 , wherein the cell attachment mediator is selected from the group consisting of collagen, elastin, fibronectin, vitronectin, laminin, proteoglycans, a peptide containing known integrin binding domains and a combination thereof.

16. The composition of claim 14 , wherein the growth factor is selected from the group consisting of fibroblast growth factor, epidermal growth factor, platelet-derived growth factor, insulin-like growth factor, transforming growth factor, and a combination thereof.

17. The composition of claim 14 , wherein the osteoinductive substance is a bone morphogenic protein.

18. The composition of claim 10 , wherein the additive is a bioactive substance that functions as a receptor or chemoattractant for a desired population of cells.

Assignees

• Tufts College

Inventors

• Kaplan David L
• Kim Ung-Jin
• Park Jaehyung
• Jin Hyoung-Joon

Classifications

• C08J9/0061

  characterized by the use of several polymeric components · CPC title

• C08J2201/0444

  Salts · CPC title

• A61L27/3604

  characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel · CPC title

• A61L27/52

  Hydrogels or hydrocolloids · CPC title

• A61L2430/02

  for reconstruction of bones; weight-bearing implants · CPC title