BSL2v2c2-Ig polypeptides

Patent metadata

Field	Value
Publication number	US-10308702-B2
Application number	US-201615218699-A
Country	US
Kind code	B2
Filing date	Jul 25, 2016
Priority date	Jun 6, 2000
Publication date	Jun 4, 2019
Grant date	Jun 4, 2019

Abstract

The present invention provides nucleic acids encoding B7-related factors that modulate the activation of immune or inflammatory response cells, such as T-cells. Also provided are expression vectors and fusion constructs comprising nucleic acids encoding B7-related polypeptides, including BSL1, BSL2, and BSL3. The present invention further provides isolated B7-related polypeptides, isolated fusion proteins comprising B7-related polypeptides, and antibodies that are specifically reactive with B7-related polypeptides, or portions thereof. In addition, the present invention provides assays utilizing B7-related nucleic acids, polypeptides, or peptides. The present invention further provides compositions of B7-related nucleic acids, polypeptides, fusion proteins, or antibodies that are useful for the immunomodulation of a human or animal subject.

First claim

What is claimed is:

1. An isolated polypeptide comprising
(a) amino acids 1 to 480 of SEQ ID NO:135;
(b) amino acids 2 to 480 of SEQ ID NO:135; or
(c) amino acids 29 to 480 of SEQ ID NO:135.

2. The isolated polypeptide of claim 1 , wherein said polypeptide is (a).

3. The isolated polypeptide of claim 2 , wherein said polypeptide is encoded by nucleotides 1 to 1440 of SEQ ID NO:134.

4. The isolated polypeptide of claim 1 , wherein said polypeptide is (b).

5. The isolated polypeptide of claim 4 , wherein said polypeptide is encoded by nucleotides 4 to 1440 of SEQ ID NO:134.

6. The isolated polypeptide of claim 1 , wherein said polypeptide is (c).

7. The isolated polypeptide of claim 6 , wherein said polypeptide is encoded by nucleotides 85 to 1440 of SEQ ID NO:134.

8. An isolated polypeptide encoded by the isolated nucleic acid in ATCC Deposit No. PTA-1987.

9. An isolated polypeptide produced by a method comprising:
(a) culturing an isolated recombinant host cell comprising a vector that comprises the coding region encoding the polypeptide of claim 1 under conditions such that said polypeptide is expressed; and (b) recovering said polypeptide.

10. The isolated polypeptide of claim 1 wherein further comprising a heterologous polypeptide sequence.

11. An isolated polypeptide comprising amino acids 1 to 247 of SEQ ID NO:135 fused to the Fc domain of an immunoglobulin.

12. An isolated polypeptide comprising amino acids 29 to 246 of SEQ ID NO:135 fused to the Fc domain of an immunoglobulin.

13. An isolated polypeptide comprising:
(a) a first sequence comprising amino acids 1 to 246 of SEQ ID NO:135 fused to a second sequence comprising the Fc domain of an immunoglobulin;
(b) a first sequence comprising amino acids 2 to 246 of SEQ ID NO:135 fused to a second sequence comprising the Fc domain of an immunoglobulin; or
(c) a first sequence comprising amino acids 29 to 246 of SEQ ID NO:135 fused to a second sequence comprising the Fc domain of an immunoglobulin.

14. The isolated polypeptide of claim 13 , wherein said first sequence of (a) comprises a sequence encoded by nucleotides 1 to 738 of SEQ ID NO:134.

15. The isolated polypeptide of claim 13 , wherein said first sequence of (b) comprises a sequence encoded by nucleotides 4 to 738 of SEQ ID NO:134.

16. The isolated polypeptide of claim 13 , wherein said first sequence of (c) comprises a sequence encoded by nucleotides 85 to 738 of SEQ ID NO:134.

17. The isolated polypeptide according to claim 11 , 12 , 13 , 14 , 15 , or 16 , wherein said immunoglobulin is IgG 1 .

18. An isolated polypeptide comprising amino acids 29 to 246 of SEQ ID NO:135.

19. The isolated polypeptide of claim 18 , wherein said polypeptide comprises a sequence encoded by nucleotides 85 to 738 of SEQ ID NO:134.

Assignees

• Bristol Myers Squibb Co
• Bristol Myers Squibb Co

Inventors

• Mikesell Glen Eugene
• Chang Han
• Peach Robert James

Classifications

• C07K16/2827

  against B7 molecules, e.g. CD80, CD86 · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• C07K2319/30

  Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title

• C07K2319/02

  containing a signal sequence · CPC title