Magnetic separation of lipoproteins using dextran sulfate

Patent metadata

Field	Value
Publication number	US-10308680-B2
Application number	US-201514985632-A
Country	US
Kind code	B2
Filing date	Dec 31, 2015
Priority date	Dec 30, 2010
Publication date	Jun 4, 2019
Grant date	Jun 4, 2019

Abstract

The invention provides apparatus and methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged-particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged-particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.

First claim

What is claimed is:

1. A method for purifying lipoproteins suitable for differential charged-particle mobility analysis of lipoprotein class and subclass, said method not including density gradient centrifugation; said method comprising:
combining glycine with a sample comprising lipoproteins to provide a glycine mixture;
adding one or more cations and dextran sulfate to the glycine mixture to form a precipitation mixture;
contacting the precipitation mixture with an insoluble solid support, wherein one or more classes or subclasses of lipoproteins from said sample precipitate from said mixture to form precipitated lipoproteins onto said insoluble solid support;
separating said precipitated lipoproteins on said insoluble solid support from said mixture; and
removing said precipitated lipoproteins from said insoluble solid support;
wherein said precipitated lipoproteins on said insoluble solid support comprise HDL and one or more selected from the group consisting of LDL, Lp(a), IDL and VLDL;
wherein said insoluble solid support comprises magnetic beads and binds precipitated lipoproteins without an immunoaffinity agent; and
wherein said dextran sulfate is not biotinylated and said precipitated lipoproteins are not bound to said insoluble solid support by streptavidin.

2. The method of claim 1 , wherein said cations comprise one or more divalent cations.

3. The method of claim 2 , wherein said one or more divalent cations are selected from the group consisting of Mg 2+ , Ca 2+ , Mn 2+ , and Sr 2+ .

4. The method of claim 2 , wherein said one or more divalent cations comprise Ca 2+ .

5. The method of claim 4 , wherein said precipitation mixture comprises Ca 2+ at a concentration within the range of about 25 mM to about 200 mM.

6. The method of claim 4 , wherein said precipitation mixture comprises Ca 2+ at a concentration of about 150 mM.

7. The method of claim 2 , wherein said one or more divalent cations comprise Mg 2+ .

8. The method of claim 7 , wherein said precipitation mixture comprises Mg 2+ at a concentration within the range of about 25 mM to about 200 mM.

9. The method of claim 7 , wherein said precipitation mixture comprises Mg 2+ at a concentration of about 100 mM.

10. The method of claim 2 , wherein said one or more divalent cations comprise Ca 2+ and Mg 2+ .

11. The method of claim 10 , wherein said precipitation mixture comprises Ca 2+ at a concentration within the range of about 25 mM to about 200 mM and Mg 2+ at a concentration within the range of about 25 mM to about 200 mM.

12. The method of claim 10 , wherein said precipitation mixture comprises Ca 2+ at a concentration of about 25 mM and Mg 2+ at a concentration of about 100 mM.

13. The method of claim 1 , wherein said dextran sulfate is in the size range of about 10 to about 500 kDa.

14. The method of claim 1 , wherein said dextran sulfate is in the size range of about 50 to about 100 kDa.

15. The method of claim 1 , wherein said dextran sulfate has a size of about 50 kDa.

16. The method of claim 1 , wherein the magnetic beads comprise a magnetic or paramagnetic core.

Assignees

• Quest Diagnostics Invest Inc

Inventors

• Caulfield Michael P
• Liu Jackie
• Shalhout Dawn
• Chen Zhihong

Classifications

• G01N27/447

  using electrophoresis · CPC title

• G01N27/622

  Ion mobility spectrometry · CPC title

• C07K1/122

  Hydrolysis with acids different from HF · CPC title

• C07K1/30 Primary

  by precipitation · CPC title

• C07K1/36

  by a combination of two or more processes of different types · CPC title