Process for preparing high-purity L-arabinose by using arabic gum as raw material

Patent metadata

Field	Value
Publication number	US-10308674-B2
Application number	US-201515550655-A
Country	US
Kind code	B2
Filing date	May 7, 2015
Priority date	Mar 24, 2015
Publication date	Jun 4, 2019
Grant date	Jun 4, 2019

Abstract

The present invention discloses a process for preparing L-arabinose from Gum Arabic comprising the steps of catalytical hydrolysis of L-arabinose from gum arabic followed by the purification steps including neutralization using alkali, adsorption bleaching, electrodialysis desalination, adsorption separation of impurities and crystallization, with the absolute purity of L-arabinose up to 98% and the recovery as high as 25%˜29% of material weight. The disclosed process has such advantages as low cost, environmental-friendliness and simple operation, showing promising in industrial production.

First claim

The invention claimed is:

1. A process for preparing high-purity L-arabinose from gum arabic, the process comprising the following steps:
(1) adding dissolved gum arabic into an inorganic acid to form a mixed solution, controlling pH value of the solution at 0.2˜1, undertaking hydrolysis reaction at the temperature of 70˜100° C., after the reaction, neutralizing the hydrolysate by adding an alkali aqueous solution to reach pH of about 4˜7 and obtaining the neutralized hydrolysate containing L-arabinose;
(2) adding the hydrolysate of step (1) to a fixed bed equipped with an adsorbent A continuously, collecting an effluent that passes through the adsorbent A to form a decolored solution;
(3) passing the decolored solution of step (2) through a microporous membrane of a drainage to obtain a pretreatment solution, then desalinating the pretreatment solution by electrodialysis to obtain a desalination solution;
(4) passing the desalination solution of step (3) through a fixed bed equipped with an adsorbent B continuously under room temperature, collecting an effluent that passes through the adsorbent B, obtaining a supersaturated syrup by concentrating the effluent that passes through the adsorbent B under reduced pressure at 45˜60° C.; and
(5) crystallizing the supersaturated syrup and obtaining white powder of L-arabinose after drying;
wherein the adsorbent A is one of granular activated carbon, non-polar macroporous polystyrene adsorption resin, weak polar macroporous polystyrene adsorption resin, non-polar macroporous acrylic adsorption resin, weak polar macroporous acrylic adsorption resin, non-polar gel-type polystyrene adsorption resin, weak polar gel-type polystyrene adsorption resin, weak polar gel-type polystyrene acid adsorption resin, non-polar gel-type acrylic adsorption resin and weak polar gel-type acrylic adsorption resin;
wherein the process of the electrodialysis consists of the steps of: circulating the pretreatment solution in concentrated solution chamber and pure water in diluted solution chamber of an electrodialysis instrument respectively through electrode chamber that contains Na 2 SO 4 as polar water and desalinating for 2˜5 hours under constant voltage and velocity;
wherein the adsorbent B is one of non-polar macroporous polystyrene adsorption resin, non-polar macroporous acrylic adsorption resin, non-polar gel-type polystyrene acid adsorption resin, non-polar gel-type acrylic adsorption resin, granular activated carbon, and silica gel bonded with C18 or C30; and
wherein the process of the crystallization comprises the steps of:
dissolving the supersaturated syrup of step (4) in alcohol, cooling the temperature to ambient temperature, inducing crystallization by adding some L-arabinose, filtering after crystallizing for 24˜72 hours, and obtaining pure L-arabinose with purity of 98% or higher after drying in a vacuum oven at 50˜60° C.;
the alcohol is methanol or ethanol, the ratio of alcohol volume to the supersaturated syrup by weight is (6˜10) L: 1 kg, the cooling rate is 0.2˜3° C./min and crystallization temperature range is −5˜10° C.

2. The process for preparing high-purity L-arabinose from gum arabic according to claim 1 , wherein the inorganic acid is sulfuric acid, hydrochloric acid, phosphoric acid or mixture of two thereof and molar concentration of H + is 1˜6 mol/L.

3. The process for preparing high-purity L-arabinose from gum arabic according to claim 1 , wherein the hydrolysis time is 1˜5 hours.

4. The process for preparing high-purity L-arabinose from gum arabic according to claim 1 , wherein decolorization temperature of step (2) is 20˜55° C. and flow rate is 0.5˜3 bed volume/hour.

5. The process for preparing high-purity L-arabinose from gum arabic according to claim 1 , wherein the mass concentration of Na 2 SO 4 is 3% ˜8% and the volume ratio of the pretreatment solution and pure water is 1: 1˜3.

6. The process for preparing high-purity L-arabinose from gum arabic according to claim 1 , wherein the desalination conditions are as follows: operating voltage is 20˜30 V and the solutions flow rate is 10˜30 L/hours.

7. A process for preparing high-purity L-arabinose from gum arabic, the process comprising the following steps:
(1) adding dissolved gum arabic into an inorganic acid to form a mixed solution, controlling pH value of the solution at 0.2˜1, undertaking hydrolysis reaction at the temperature of 70˜100° C., after the reaction, neutralizing the hydrolysate by adding an alkali aqueous solution to reach pH of about 4˜7 and obtaining the neutralized hydrolysate containing L-arabinose;
(2) adding the hydrolysate of step (1) to a fixed bed equipped with an adsorbent A continuously, collecting an effluent that passes through the adsorbent A to form a decolored solution;
(3) passing the decolored solution of step (2) through a microporous membrane of a drainage to obtain a pretreatment solution, then desalinating the pretreatment solution by electrodialysis to obtain a desalination solution;
(4) passing the desalination solution of step (3) through a fixed bed equipped with an adsorbent B continuously under room temperature, collecting an effluent that passes through the adsorbent B, obtaining a supersaturated syrup by concentrating the effluent that passes through the adsorbent B under reduced pressure at 45˜60° C.; and
(5) crystallizing the supersaturated syrup and obtaining white powder of L-arabinose after drying;
wherein the adsorbent A is one of granular activated carbon, non-polar macroporous polystyrene adsorption resin, weak polar macroporous polystyrene adsorption resin, non-polar macroporous acrylic adsorption resin, weak polar macroporous acrylic adsorption resin, non-polar gel-type polystyrene adsorption resin, weak polar gel-type polystyrene adsorption resin, weak polar gel-type polystyrene acid adsorption resin, non-polar gel-type acrylic adsorption resin and weak polar gel-type acrylic adsorption resin;
wherein the process of the electrodialysis consists of the steps of: circulating the pretreatment solution in concentrated solution chamber and pure water in diluted solution chamber of an electrodialysis instrument respectively through electrode chamber that contains Na 2 SO 4 as polar water and desalinating for 2˜5 hours under constant voltage and velocity;
wherein the adsorbent B is one of non-polar macroporous polystyrene adsorption resin, non-polar macroporous acrylic adsorption resin, non-polar gel-type polystyrene acid adsorption resin, non-polar gel-type acrylic adsorption resin, granular activated carbon, and silica gel bonded with C18 or C30;
wherein the process of the crystallization comprises the steps of:
dissolving the supersaturated syrup of step (4) in alcohol, cooling the temperature to ambient temperature, inducing crystallization by adding some L-arabinose, filtering after crystallizing for 24˜72 hours, and obtaining pure L-arabinose with purity of 98% or higher after drying in a vacuum oven at 50˜60° C.;
the alcohol is methanol or ethanol, the ratio of alcohol volume to the supersaturated syrup by weight is (6˜10) L: 1 kg, the cooling rate is 0.2˜3° C./min and crystallization temperature range is -5˜10° C.;
wherein the inorganic acid is sulfuric acid, hydrochloric acid, phosphoric acid or mixture of two thereof and molar concentration of H + is 1˜6 mol/L;
wherein the hydrolysis time is 1˜5 hours;
wherein decolorization temperature of step (2) is 20˜55° C. and flow rate is 0.5˜3 bed volume/hour;
wherein the mass concentration of Na 2 SO 4 is 3% -˜8% and the volume ratio of the pretreatment solution and pure water is 1: 1˜3; and
wherein the desalination conditions are as follows: operating voltage is 20˜30 V and the solutions flow rate is 10˜30 L/hours.

Assignees

• Univ Zhejiang

Inventors

• Bao Zongbi
• Huang Minhui
• Zhang Zhiguo
• Yang Qiwei
• Su Baogen
• Xing Huabin
• Ren Qilong
• Yang Yiwen

Classifications

• C07H3/02 Primary

  Monosaccharides · CPC title

• C07H1/08

  from natural products · CPC title