Methods, compositions, and delivery systems for therapeutic skin treatments
US-2024390236-A1 · Nov 28, 2024 · US
US10307347B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10307347-B2 |
| Application number | US-201715412114-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 23, 2017 |
| Priority date | Feb 5, 2016 |
| Publication date | Jun 4, 2019 |
| Grant date | Jun 4, 2019 |
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A bio cellulose membrane with an outstanding water retention capacity and a method for producing the same are provided. The bio cellulose membrane comprises a first surface, a second surface, and a loose layer, wherein the average pore size of the first surface and the average pore size of the second surface are smaller than that of the loose layer.
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What is claimed is: 1. A bio cellulose membrane having a water retention capacity, comprising a first surface, a second surface opposite to the first surface, and a loose layer between the first surface and second surface, wherein the first surface, the second surface and the loose layer are made of the same material produced from a microbial culture of acetic acid microorganism, the average pore size of the first surface and the average pore size of the second surface independently range from 20 to 80 nm, the average pore size of the loose layer ranges from 50 to 150 nm, and the average pore size of the first surface and the average pore size of the second surface are smaller than that of the loose layer. 2. A method for producing the bio cellulose membrane of claim 1 , comprising: culturing the acetic acid microorganism with a temperature gradient process such that the microorganism is cultured under the following temperature sequence: a first low temperature, a high temperature, and a second low temperature; collecting the bio cellulose membrane naturally produced from the culture of the microorganism; wherein the first low temperature and the second low temperature independently range from 20° C. to 25° C.; and wherein the high temperature ranges from 30° C. to 35° C. 3. The method of claim 2 , wherein the microorganism is Gluconacetobacter xylinus. 4. The method of claim 2 , wherein the first low temperature and the second low temperature are the same or different. 5. The method of claim 2 , wherein the culture duration at the first low temperature, the culture duration at the high temperature, and the culture duration at the second low temperature are in the ratio of 1:1:2. 6. The method of claim 5 , wherein the culture duration at the first low temperature and the culture duration at the second low temperature are 18 hours respectively. 7. The method of claim 5 , wherein the culture duration at the high temperature is 36 hours.
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