Reaction container and method for producing same

Patent metadata

Field	Value
Publication number	US-10301064-B2
Application number	US-201114000832-A
Country	US
Kind code	B2
Filing date	Aug 19, 2011
Priority date	Feb 22, 2011
Publication date	May 28, 2019
Grant date	May 28, 2019

Abstract

The reaction container is configured to comprise: one or two or more housing parts for reactions comprising: a narrow-mouthed piping part in which a reaction reagent or a portion thereof is housed or is housable; a wide-mouthed piping part that is communicated with the narrow-mouthed piping part and provided on an upper side of the narrow-mouthed piping part, and has an aperture that is wider than an aperture of the narrow-mouthed piping part; and a punchable film provided such that it partitions an interval between the wide-mouthed piping part and the narrow-mouthed piping part.

First claim

The invention claimed is:

1. A reaction container comprising:
one or two or more housing parts for reactions comprising: a narrow-mouthed piping part formed with a rotation symmetry and in which a reaction reagent or a portion thereof is housed or is housable; a wide-mouthed piping part that is communicated with the narrow-mouthed piping part and provided on an upper side of the narrow-mouthed piping part, the wide-mouthed piping part being formed with a rotation symmetry coaxially with the narrow-mouthed piping part and having an aperture that is wider than an aperture of said narrow-mouthed piping part; and a punchable film provided such that it partitions an interval between the wide-mouthed piping part and the narrow-mouthed piping part, the punchable film, when punched, being insertable into the aperture of said narrow-mouthed piping part; and
a sealing lid that seals the reaction container by being mounted on the aperture of said wide-mouthed piping part of said reaction container, said sealing lid comprising: a plug portion defining an internal cavity and an exterior bottom surface having transparency, wherein the plug portion is fittable to an inside wall defined by the aperture of said wide-mouthed piping part and is able to guide light from said narrow-mouthed piping part; and a pushing portion provided to the exterior bottom surface of said plug portion so that, after the film has been punched and during the fitting of the plug portion with the wide-mouthed piping part, the pushing portion pushes said film, which has been already punched, into the aperture of the narrow-mouthed piping part, and against an inner wall of the narrow-mouthed piping part, thus enabling the plug portion to guide light from said narrow-mouthed piping part;
wherein the pushing portion comprises a cylindrical wall extending vertically downward from the exterior bottom surface of the sealing lid and formed so as to circularly enclose a center portion, through which the light guided by the sealing lid passes, adjacent the exterior bottom surface, which exterior bottom surface encloses the center portion at one end of the cylindrical wall to separate the center portion from the internal cavity of the plug portion;
wherein, when the plug portion is fitted to the inside wall defined by the aperture of the wide-mouthed piping part, at least a portion of the pushing portion extends downward into the narrow-mouthed piping part and an annular region is defined between an exterior surface of the cylindrical wall of the pushing portion and the inner wall of the narrow-mouthed piping part, so that no portion of the exterior surface contacts the inner wall at any location between the exterior bottom surface of the plug portion and a bottom end of the pushing portion; and
wherein the cylindrical wall of the pushing portion extends vertically downward from the exterior bottom surface of the sealing lid by a vertical length that is longer than a radial length of the annular region so that, when the plug portion is fitted to the inside wall defined by the aperture of the wide-mouthed piping part, the cylindrical wall of the pushing portion extends downward to push said punched film into the aperture of the narrow-mouthed piping part and against the inner wall of the narrow-mouthed piping part.

2. A reaction container according to claim 1 , in which the aperture of said narrow-mouthed piping part is provided at the center of a bottom portion of the wide-mouthed piping part.

3. A reaction container according to claim 1 , wherein said narrow-mouthed piping part and said wide-mouthed piping part are integrally formed, and said film is attached to a bottom portion of said wide-mouthed piping part.

4. A reaction container according to claim 1 that has a cartridge container which further has a base plate in which two or more concave portions are arranged in a single row form, and said housing part for reactions is formed in one of the concave portions, and in the other of the concave portions excluding the concave portion in which said housing part for reactions is formed, instruments for performing processing that are moved to said housing part for reactions are housed or are housable.

5. A reaction container according to claim 4 , wherein said other concave portions excluding the concave portion in which said housing part for reactions is formed, are provided with a sealing lid housing part that houses said sealing lid, a tip for punching housing part that houses a tip for punching that punches said film, and/or a dispensing tip housing part that houses a dispensing tip.

6. A reaction container according to claim 4 , wherein said wide mouth piping part of said container for reaction formed in said concave portion comprises a concave portion having an aperture formed in said base plate, and said narrow-mouthed piping part is separately formed from said wide-mouthed piping part, a hole portion is piercingly provided in the center of a bottom portion of the wide-mouthed piping part, said narrow-mouthed piping part has an aperture edge portion that encloses the aperture thereof, said narrow-mouthed piping part downwardly protrudes from said hole portion of the wide-mouthed piping part such that it passes through said hole portion, said aperture edge portion is installed on the bottom portion of said wide-mouthed piping part, and said film is attached to said aperture edge portion of said narrow-mouthed piping part.

7. A reaction container according to claim 1 , wherein said wide-mouthed piping part or said sealing lid are adapted to be able to be linked with a linking portion, the linking portion including ends of one or two or more light guide portions so that, when the linking portion is linked with said wide-mouthed piping part or said sealing lid, the light guide portions optically connect a light measuring device provided on the exterior of the reaction container with the interior of the housing part for reactions.

8. A reaction container according to claim 1 , wherein said sealing lid has: a cavity provided in the center thereof; and a bottom surface, which has transparency, that blocks a lower end of the cavity, and movement of the sealing lid to the housing part for reactions, and the fitting and/or the linking with said linking portion, is performed by inserting a member provided on the exterior of the reaction container and/or said linking portion into said cavity.

9. A reaction container comprising:
one or two or more housing parts for reactions comprising: a narrow-mouthed piping part formed with a rotation symmetry and in which a reaction reagent or a portion thereof is housed or is housable; a wide-mouthed piping part that is communicated with the narrow-mouthed piping part and provided on an upper side of the narrow-mouthed piping part, the wide-mouthed piping part being formed with a rotation symmetry coaxially with the narrow-mouthed piping part and having an aperture that is wider than an aperture of said narrow-mouthed piping part; and a punchable film provided such that it partitions an interval between the wide-mouthed piping part and the narrow-mouthed piping part, the punchable film, when punched, being insertable into the aperture of said narrow-mouthed piping part; and
a sealing lid that seals the reaction container by being mounted on the aperture of said wide-mouthed piping part of said reaction container, said sealing lid comprising: a plug portion defining an internal cavity and an exterior bottom surface having transparency, wherein the plug portion is fittable to an inside wall defined by the aperture of said wide-mouthed piping part and is able to guide light from said narrow-mouthed piping part; and a pushing portion provided to the exterior bottom surface of said

Assignees

• Tajima Hideji
• Universal Bio Research Co Ltd

Inventors

• Tajima Hideji

Classifications

• B01L2200/028

  Modular arrangements · CPC title

• B65D7/12 Primary

  characterised by wall construction or by connections between walls · CPC title

• G01N2201/0826

  Fibre array at source, distributing · CPC title

• B01L2300/0832

  cylindrical, tube shaped · CPC title

• B01L3/50855

  using modular assemblies of strips or of individual wells · CPC title