Pipetting device for an apparatus for processing a sample or reagent, apparatus for processing a sample or reagent and method for pipetting a sample or reagent

Patent metadata

Field	Value
Publication number	US-10300480-B2
Application number	US-201615291960-A
Country	US
Kind code	B2
Filing date	Oct 12, 2016
Priority date	Oct 13, 2015
Publication date	May 28, 2019
Grant date	May 28, 2019

Abstract

A pipetting device ( 100 ) for an apparatus ( 164 ) for processing a sample or reagent is disclosed. The pipetting device ( 100 ) comprises a coupling mechanism ( 102 ), wherein the coupling mechanism ( 102 ) comprises at least a first coupling unit ( 104 ) adapted to be coupled to a first pipetting tip and a second coupling unit ( 106 ) adapted to be coupled to a second pipetting tip, and a tilt mechanism ( 112 ) for moving the first coupling unit ( 104 ) between an untilted position, in which the first coupling unit ( 104 ) and the second coupling unit ( 106 ) are arranged parallel to one another, and a tilted position, in which the first coupling unit ( 104 ) is tilted relative to the second coupling unit ( 106 ).

First claim

The invention claimed is:

1. A pipetting device for an apparatus for processing a sample or reagent, comprising a frame including the following elements arranged within and supported by the frame:
a coupling mechanism including at least a first coupling unit adapted to be coupled to a first pipetting tip and a second coupling unit adapted to be coupled to a second pipetting tip,
a tilt mechanism configured to move the first coupling unit between an untilted position, in which the first coupling unit and the second coupling unit are arranged parallel to one another, and a tilted position, in which the first coupling unit is tilted relative to the second coupling unit, and
a tilt mechanism trigger adapted to trigger the tilt mechanism by engagement with an activation device,
wherein movement of the pipetting device relative to the activation device engages with and activates the tilt mechanism, fixing the tilt mechanism in the untilted position, and thereby fixing the first coupling unit in the untilted position.

2. The pipetting device of claim 1 , wherein the tilt mechanism is tiltable around a first pivot.

3. The pipetting device of claim 2 , wherein the tilt mechanism comprises a recess and the tilt mechanism trigger comprises a pin and upon engagement of the tilt mechanism trigger, the pin engages with the recess, thereby fixing the tilt mechanism in the untilted position.

4. The pipetting device of claim 1 , wherein the tilt mechanism is releasably fixed in the untilted position.

5. The pipetting device of claim 1 , wherein the tilt mechanism is releasably fixed in the tilted position.

6. The pipetting device of claim 2 , wherein the tilt mechanism comprises a second recess engageable with a protrusion of the activation device, wherein the tilt mechanism is tiltable around the first pivot by means of a movement of the pipetting device relative to the activation device.

7. The pipetting device of claim 1 , further comprising a sensor for detecting whether the first coupling unit is in the untilted position or the tilted position.

8. An apparatus for processing a sample or reagent, comprising a pipetting device of claim 1 , an input for a first vessel, said first vessel comprising a sample or reagent, and a holder for holding a second vessel to which the sample or reagent is transferrable by the pipetting device.

9. An apparatus for processing a sample or reagent of claim 8 , further comprising an activation device adapted to activate the tilt mechanism of the pipetting device.

10. A method for pipetting a sample or reagent using a pipetting device according to claim 1 , comprising:
(a) coupling a second pipetting tip to the second coupling unit,
(b) moving the first coupling unit from the untilted position, in which the first coupling unit and the second coupling unit are arranged parallel to one another, into the tilted position, in which the first coupling unit is tilted relative to the second coupling unit, and
(c) aspirating the sample or reagent from a first vessel by means of the second pipetting tip while the first coupling unit is in the tilted position.

11. A method of claim 10 , wherein the first coupling unit is moved into the tilted position if a ratio of a length of the second pipetting tip and a target immersion depth of the second pipetting tip into the first vessel is below a predetermined threshold.

12. A method of claim 10 , wherein the tilt mechanism of the pipetting device is activated upon engagement with an activation device.

13. A method of claim 10 , further comprising transferring the sample or reagent to a second vessel using the pipetting device.

Assignees

• Roche Molecular Systems Inc

Inventors

• Bachmann Hans-Rudolf
• Schneebeli Rolf

Classifications

• B01L9/543

  for disposable pipette tips, e.g. racks or cassettes · CPC title

• G01N35/1009

  Characterised by arrangements for controlling the aspiration or dispense of liquids · CPC title

• G01N2035/1025

  Fluid level sensing · CPC title

• B01L3/0279 Primary

  co-operating with positive ejection means · CPC title

• G01N35/1072

  with provision for selective pipetting of individual channels · CPC title