Extract of Vicia faba beans

Patent metadata

Field	Value
Publication number	US-10300100-B1
Application number	US-201816145090-A
Country	US
Kind code	B1
Filing date	Sep 27, 2018
Priority date	Sep 27, 2018
Publication date	May 28, 2019
Grant date	May 28, 2019

Abstract

The extract of Vicia faba beans is prepared by soaking beans in distilled water overnight and then boiling in a water bath to reduce the volume of aqueous extract, which is then homogenized and filtered. The filtrate is concentrated to a smaller volume, lyophilized, and powdered. The lyophilized powder is extracted with hexane to remove oils and lipids. The oil-free lyophilized powder is dissolved in ethanol solvent and extracted for eight hours under reflux, and filtered. The volume of ethanol is reduced by a rotary evaporator, and a first off-white precipitate (sample A-1) is collected, washed with ethanol, and dried at 80° C. Mass spectrometry shows a molecular weight of 200.16447 g mol−1, and an empirical formula of C9H16N2O3 is assigned. Intraperitoneal injection of mice with 50 mg/kg of A-1 twenty minutes prior to injection with strychnine protected the mice from strychnine-induced convulsions to the same extent as phenobarbitone (phenobarbital).

First claim

We claim:

1. A method of producing an anticonvulsant extract of Vicia faba beans, comprising the steps of:
extracting Vicia faba beans in water to obtain an aqueous extract;
lyophilizing the aqueous extract and powdering the lyophilized aqueous extract to obtain a lyophilized powder;
extracting the lyophilized powder in hexane to remove lipids and obtain an oil-free lyophilized powder;
extracting the oil-free lyophilized powder in ethanol solvent under reflux and filtering off the ethanol solvent;
removing the ethanol solvent by rotary evaporator to leave a precipitate;
washing the precipitate in ethanol; and
drying the precipitate at 80° C.

2. The method of producing an anticonvulsant extract of Vicia faba beans according to claim 1 , wherein the Vicia faba beans are a cultivar of the Sudan.

3. The method of producing an anticonvulsant extract of Vicia faba beans according to claim 1 , wherein the step of extracting Vicia faba beans in water comprises the steps of:
soaking the beans in distilled water overnight to obtain the aqueous extract;
boiling the water-soaked beans in a water bath until the aqueous extract is reduced in volume by at least two-thirds;
homogenizing the aqueous extract; and
filtering the homogenized aqueous extract, retaining the filtrate.

4. The method of producing an anticonvulsant extract of Vicia faba beans according to claim 3 , wherein the step of lyophilizing the aqueous extract comprises lyophilizing the filtrate.

5. The method of producing an anticonvulsant extract of Vicia faba beans according to claim 1 , wherein the step of extracting the lyophilized powder in hexane comprises extracting the lyophilized powder in hexane in a Soxhlet extractor.

6. The method of producing an anticonvulsant extract of Vicia faba beans according to claim 1 , wherein the step of extracting the oil-free lyophilized powder in ethanol solvent under reflux comprises heating the oil-free lyophilized powder in ethanol solvent under reflux for about eight hours.

7. The method of producing an anticonvulsant extract of Vicia faba beans according to claim 1 , wherein the dried precipitate has a molar mass of 200.16447 g/mol.

8. The method of producing an anticonvulsant extract of Vicia faba beans according to claim 1 , wherein the dried precipitate has an empirical formula of C 9 H 16 N 2 O 3 .

Assignees

• Univ King Saud

Inventors

• Khalil Mutasim Ibrahim
• Salih Mustafa Abdalla Mohamed
• Ali Ali Ahmed Mustafa

Classifications

• A61K2236/53

  Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization · CPC title

• A61K2236/51

  Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying · CPC title

• A61K2236/39

  Complex extraction schemes, e.g. fractionation or repeated extraction steps · CPC title

• A61K2236/35

  Extraction with lipophilic solvents, e.g. Hexane or petrol ether · CPC title

• A61K2236/333

  using mixed solvents, e.g. 70% EtOH · CPC title