Inhibiting or reducing fungal infections

Patent metadata

Field	Value
Publication number	US-10300093-B2
Application number	US-201515317814-A
Country	US
Kind code	B2
Filing date	Jun 10, 2015
Priority date	Jun 10, 2014
Publication date	May 28, 2019
Grant date	May 28, 2019

Abstract

Provided are methods and compositions for inhibiting or reducing fungal growth in or on a subject. The methods comprise exposing the subject to a composition comprising one or more enzymes, one or more bacteria, and/or an enzymatic extract, wherein the one or more enzymes, one or more bacteria, and/or the enzymatic extract isolated from one or more bacteria are exposed to the subject in a quantity sufficient to inhibit or reduce fungal growth.

First claim

What is claimed is:

1. A method for treating or preventing fungal infection in an animal subject, comprising placing a composition comprising one or more bacteria in the proximity of, but not directly on, the subject, wherein the one or more bacteria are selected from the group consisting of Rhodococcus rhodochrous DAP 96253, Rhodococcus rhodochrous DAP 96622, Rhodococcus erythropolis , and combinations thereof, and wherein the one or more bacteria are provided in a quantity sufficient to inhibit or reduce fungal growth in the subject.

2. The method of claim 1 , wherein the one or more bacteria are induced to produce one or more enzymes selected from the group consisting of nitrile hydratases, amidases, asparaginases, ACC deaminases, monooxygenases, dioxygenases, cyanidases, and combinations thereof.

3. The method of claim 2 , wherein the composition further comprises the one or more enzymes produced by the one or more bacteria.

4. The method of claim 3 , wherein the composition further comprises one or more cofactors for the one or more enzymes.

5. The method of claim 1 , wherein the composition further comprises an inducing agent selected from the group consisting of urea, methyl carbamate, methacrylamide, acetamide, cobalt, asparagine, anhydrous asparagine, asparagine monohydrate, and combinations thereof.

6. The method of claim 5 , wherein the inducing agent comprises urea or methyl carbamate and one or more of cobalt and asparagine.

7. The method of claim 1 , wherein the composition further comprises a stabilizing agent.

8. The method of claim 7 , wherein the stabilizing agent is trehalose.

9. The method of claim 1 , wherein the one or more bacteria are fixed with glutaraldehyde and cross-linked.

10. The method of claim 1 , wherein the one or more bacteria are provided in a coating layer.

11. The method of claim 10 , wherein the coating layer is selected from a hydrophobic fatty acid polyester coating or a wax.

12. The method of claim 1 , wherein the composition is provided in a liquid carrier, solid carrier, aerosol, or gel.

13. The method of claim 1 , wherein the subject is a bat, wherein the fungal infection comprises Pseudogymnoascus destructans.

14. The method of claim 13 , wherein the composition is applied to areas in or around a bat roost.

15. The method of claim 13 , wherein the composition is provided in a bat lure.

16. The method of claim 1 , wherein the subject is a honey bee, wherein the fungal infection is selected from the group consisting of Ascosphaera apis, Nosema apis, Aspergillus fumigatus, Aspergillus flavus , and Aspergillus niger.

17. The method of claim 16 , wherein the composition is provided in a bait particle.

18. The method of claim 16 , wherein the composition is provided in a wax.

19. The method of claim 18 , wherein the wax is incorporated into a bee hive.

20. The method of claim 16 , wherein the composition is provided in a powder.

21. The method of claim 20 , wherein the powder is mixed with powdered sugar and applied as a dust to a bee hive.

22. The method of claim 16 , wherein the composition is incorporated into a material at an entrance to a bee hive.

Assignees

• Georgia State Univ And Research Foundation Inc
• Univ Georgia State Res Found

Inventors

• Pierce George E
• Crow Jr Sidney A
• Tucker Trudy Ann
• Cornelison Christopher T

Classifications

• A61K45/06

  Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca · CPC title

• A61K35/74 Primary

  Bacteria (therapeutic use of a bacterial protein A61K38/00) · CPC title

• A61K47/26

  Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin · CPC title

• A01N63/00

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title

• A01N25/12

  Powders or granules (A01N25/26 takes precedence) · CPC title