Serology assays

Patent metadata

Field	Value
Publication number	US-10295539-B2
Application number	US-201113642347-A
Country	US
Kind code	B2
Filing date	Apr 18, 2011
Priority date	Apr 19, 2010
Publication date	May 21, 2019
Grant date	May 21, 2019

Abstract

The invention provides methods and kits for measuring the ability of a test sample to inhibit the binding of a receptor expressed by a pathogen to a host cell ligand of the pathogen.

First claim

The invention claimed is:

1. A kit for measuring a sample's inhibition of a binding of a sialic acid receptor expressed by a pathogen to a host cell ligand of said pathogen, wherein said host cell ligand is sialic acid, said kit comprising:
(a) a first container, vessel or compartment comprising a first surface comprising said sialic acid receptor immobilized thereto; and
(b) a second container, vessel or compartment, the second container containing within a cavity of the second container a particle reagent comprising said sialic acid, wherein said sialic acid is bound only to the particle reagent and not the second container.

2. The kit of claim 1 wherein said first surface comprises a patterned array of receptors immobilized thereto.

3. The kit of claim 2 wherein said array of receptors is one selected from the group consisting of different strains, subtypes, types, and organisms.

4. The kit of claim 1 wherein said kit further comprises one or more control samples having known inhibitory abilities.

5. The kit of claim 4 wherein said one or more control samples include a negative control sample having no inhibitory component.

6. The kit of claim 4 wherein said one or more control samples include a positive control sample having a defined concentration of an inhibitory component.

7. The kit of claim 1 wherein said receptor is a sialic acid receptor from a virus selected from the group consisting of human coronavirus, bovine coronavirus, mouse hepatitis virus, equine rhinitis A, Influenza A virus, Influenza B virus, and Influenza C virus, Newcastle Disease Virus, murine parvovirus minute virus, reovirus, rotavirus host cell invasion, bluetongue virus, bovine adenovirus serotype 3.

8. The kit of claim 7 wherein said virus is an Influenza virus.

9. The kit of claim 1 wherein said particle reagent comprises a biological reagent selected from the group consisting of red blood cells, red blood cell vesicles, red blood cell ghosts, membrane fragments, membrane vesicles, proteins, and combinations thereof.

10. The kit of claim 1 wherein said particle reagent is cross-linked.

11. The kit of claim 10 wherein said particle reagent comprises a coating comprising said biological reagent.

12. The kit of claim 11 wherein said particle reagent is selected from the group consisting of colloids, beads, and combinations thereof.

13. The kit of claim 12 wherein said particle reagent is comprised of a substance selected from the group consisting of polystyrene, polyacrylamide, polypropylene, and latex particles, silica, alumina, carbon fibrils, and combinations thereof.

14. The kit of claim 12 wherein said particle reagent is magnetic.

15. The kit of claim 12 wherein said particle reagent is comprised of a conductive and/or semiconductive material.

16. The kit of claim 15 wherein said particle reagent comprises colloidal gold particles.

17. The kit of claim 1 wherein said receptor is provided in a form selected from the group consisting of whole virus, virus-like particles, purified hemagglutinins, recombinant hemagglutinins, purified neuraminidases, recombinant neuraminidases, membrane fragments, membrane vesicles, and combinations thereof.

18. The kit of claim 1 wherein said particle reagent comprises a detectable label.

19. The kit of claim 1 further comprising a secondary binding reagent comprising a detectable label.

20. The kit of claim 19 wherein said secondary binding reagent is an antibody.

21. The kit of claim 1 further comprising a detectable label.

22. The kit of claim 19 wherein said detectable label is an electrochemiluminescence (ECL) label.

23. The kit of claim 22 wherein said first surface comprises an electrode.

24. The kit of claim 9 wherein said biological reagent is mucin.

25. The kit of claim 1 wherein components (a)-(b) are contained within an assay cartridge.

26. The kit of claim 1 wherein components (a)-(b) are contained within a multi-well plate.

27. The kit of claim 1 wherein one or more of said components are provided in said kit as a reconstitutable dry reagent.

28. The kit of claim 27 , wherein said dry reagent and said first surface are located in a well of a multi-well plate and said dry reagent is one selected from the group consisting of
(i) free standing;
(ii) located in a surface of said first surface that does not overlap with said receptor; and
(iii) located on a ledge within said well.

Assignees

• Sigal George
• Meso Scale Technologies Llc

Inventors

• Sigal George

Classifications

• G01N2500/02

  Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor) · CPC title

• G01N33/566

  using specific carrier or receptor proteins as ligand binding reagents {where possible specific carrier or receptor proteins are classified with their target compounds} · CPC title

• G01N33/56983 Primary

  Viruses · CPC title

• G01N33/54313

  the carrier being characterised by its particulate form · CPC title