In situ NMR parameter monitoring systems and methods for measuring pH and temperature

Patent metadata

Field	Value
Publication number	US-10295487-B2
Application number	US-201514884686-A
Country	US
Kind code	B2
Filing date	Oct 15, 2015
Priority date	Oct 15, 2014
Publication date	May 21, 2019
Grant date	May 21, 2019

Abstract

Devices and methods are provided for measuring temperatures and pHs of a sample in situ using NMR spectroscopy, and for sealing one or more ends of a capillary tube after a reference material has been added to the capillary tube, which is used in an in situ NMR temperature measurement device. A method for measuring a pH of a sample in situ using NMR spectroscopy includes providing an in situ NMR pH measurement device. This device includes a sample housing member configured to house a target sample, at least one pH sensor configured to exhibit an NMR spectral change due to a change in pH value of the target sample, and a pH sensor containment member configured to house the at least one pH sensor. The target sample is added to the sample housing member. NMR spectra are obtained to then determine the pH of the target sample.

First claim

What is claimed is:

1. A device for in situ pH monitoring of a sample using Nuclear Magnetic Resonance (NMR) spectroscopy, the device comprising:
a sample housing member configured to house a target sample;
at least one pH sensor configured to exhibit an NMR spectral change due to a change in pH value of the target sample; and
a pH sensor containment member having an interior portion, the pH sensor containment member configured to maintain the at least one pH sensor in the interior portion, where the pH sensor containment member is positioned inside at least a portion of the sample housing member, wherein at least a portion of the pH sensor containment member comprises one or more pores, wherein the one or more pores are configured to allow bidirectional diffusion of hydronium cations and hydroxide anions while maintaining the at least one pH sensor in the interior portion and while precluding direct interaction of the at least one pH sensor and the target sample.

2. The device of claim 1 , wherein the at least one pH sensor exhibits a peak volume change induced by pH value changes in a sample environment.

3. The device of claim 1 , wherein the at least one pH sensor exhibits a peak chemical shift induced by pH value changes in a sample environment.

4. The device of claim 1 , wherein the pH sample containment member is any high surface area fiber or thin rod that immobilizes pH sensor molecules by entrapment or by physical tethers, the high surface area fiber or the thin rod allowing for a bidirectional transport of one or more of the hydronium cations or the hydroxide anions while impeding a transport of a target sample molecule and a pH sensor molecule.

5. The device of claim 1 , wherein the at least one pH sensor is configured to produced NMR signals from nuclei other than nuclei that produce NMR spectra of the target sample.

6. The device of claim 1 , wherein the pH sensor containment member is comprised of glass.

7. A method for measuring a pH of a sample in situ using Nuclear Magnetic Resonance (NMR) spectroscopy, the method comprising:
providing an in situ NMR pH measurement device, the device comprising:
(1) a sample housing member configured to house a target sample,
(2) at least one pH sensor configured to exhibit an NMR spectral change due to a change in pH value of the target sample, and
(3) a pH sensor containment member having an interior portion, the pH sensor containment member configured to house the at least one pH sensor, wherein at least a portion of the pH sensor containment member comprises one or more pores, wherein the one or more pores are configured to allow bidirectional diffusion of hydronium cations and hydroxide anions while maintaining the at least one pH sensor in the interior portion and while precluding direct interaction of the at least one pH sensor and the target sample;
adding the target sample to the sample housing member;
obtaining one or more NMR spectra; and determining the pH of at least a portion of the target sample.

8. The method of claim 7 , further comprising identifying a type of pH sensor to use in the in situ NMR pH measurement device.

9. The method of claim 7 , wherein the at least one pH sensor is configured to produced NMR signals from nuclei other than nuclei that produce NMR spectra of the target sample.

10. The method of claim 7 , wherein the pH sensor containment member is comprised of glass.

Assignees

• Univ Missouri

Inventors

• Huang Ming
• Chi Lingyu
• Gerald Ii Rex E
• Huang Jie
• Pfaff Annalise R
• Woelk Klaus

Classifications

• G01R33/31

  Temperature control thereof · CPC title

• G01R33/4804

  Spatially selective measurement of temperature or pH · CPC title

• G01R33/307

  specially adapted for moving the sample relative to the MR system, e.g. spinning mechanisms, flow cells or means for positioning the sample inside a spectrometer · CPC title

• G01K7/36

  using magnetic elements, e.g. magnets, coils (magnetic elements per se H01F) · CPC title

• G01N24/08 Primary

  by using nuclear magnetic resonance (G01N24/12 takes precedence) · CPC title