Eimeria tenella elongation factor-1 alpha recombinant immunogenic compositions which induce active protective immunity against avian coccidiosis

Patent metadata

Field	Value
Publication number	US-10294483-B2
Application number	US-201815887130-A
Country	US
Kind code	B2
Filing date	Feb 2, 2018
Priority date	Feb 13, 2017
Publication date	May 21, 2019
Grant date	May 21, 2019

Abstract

Provided herein are immunogenic compositions containing recombinant proteins capable of presenting all, or antigenic portions of, the Eimeria tenella Elongation Factor 1 alpha, or EF-1α, protein in the development of active immunity to, and control of, coccidiosis. Also provided are methodologies of using the immunogenic compositions for administration to poultry and other animals in the control of coccidiosis. In some instances, the EF-1α protein utilized in the immunogenic composition presented herein is molecularly manipulated or combined with adjuvants to increase effectiveness.

First claim

What is claimed is:

1. An immunogenic composition, comprising a pharmaceutically or veterinarily acceptable carrier and a recombinant protein of SEQ ID NO: 2, wherein said immunogenic composition is capable of inducing an immune response to said protein in a recipient.

2. The immunogenic composition of claim 1 , further comprising an adjuvant.

3. The immunogenic composition of claim 1 , wherein the protein is expressed by a recombinant host cell comprising an exogenous nucleic acid encoding the protein.

4. The immunogenic composition of claim 3 , wherein the host cell is an Escherichia coli cell.

5. The immunogenic composition of claim 1 , wherein the carrier is a liquid carrier.

6. The immunogenic composition of claim 1 , wherein the composition is formulated for parenteral delivery.

7. The immunogenic composition of claim 1 , wherein the composition is formulated for oral delivery.

8. The immunogenic composition of any of claims 1 - 7 , wherein the protein is an isolated protein.

9. A method of protecting a recipient against coccidiosis, comprising administering to the recipient an immunogenic composition according to claim 1 or claim 2 in an amount effective to induce a protective immune response to an Eimeria species.

10. The method of claim 9 , wherein the recipient is a chicken or turkey.

11. The method of claim 9 , wherein the immunogenic composition is administered to the recipient at a live whole-cell formulation at a dose of at least 50μg.

12. The method of claim 9 , wherein the composition is administered parenterally.

13. The method of claim 12 , wherein the composition is administered intramuscularly.

14. The method of claim 9 , wherein the composition is administered orally.

Assignees

• Us Agriculture

Inventors

• Lillehoj Hyun S
• Oh Sungtaek
• Panebra Alfredo

Classifications

• C12N15/70 Primary

  Vectors or expression systems specially adapted for E. coli · CPC title

• C07K14/44

  from protozoa · CPC title

• A61P33/02 Primary

  Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis · CPC title

• A61K2039/552

  Veterinary vaccine · CPC title

• A61K39/012

  Coccidia antigens · CPC title