Methods of using extracts of melissa officinalis against filoviruses

Patent metadata

Field	Value
Publication number	US-10293012-B2
Application number	US-201815970222-A
Country	US
Kind code	B2
Filing date	May 3, 2018
Priority date	May 4, 2017
Publication date	May 21, 2019
Grant date	May 21, 2019

Abstract

The present disclosure relates to methods of using Melissa officinalis extracts in inhibiting viral replication of a filovirus and in treating or preventing a filovirus infection.

First claim

What is claimed:

1. A method of inhibiting viral replication of a filovirus, the method comprising exposing the filovirus or a filovirus-infected cell to an effective amount of an extract from Melissa officinalis , or a composition comprising the extract, wherein the exposing inhibits viral replication within the cell.

2. A method of treating filovirus infection of a cell, the method comprising exposing a filovirus or filovirus-infected cell to an effective amount of an extract from Melissa officinalis , or a composition comprising the extract, wherein the exposing inhibits the entry of the filovirus into the cell.

3. A method of preventing filovirus infection of a cell, the method comprising exposing the cell to an effective amount of an extract from Melissa officinalis , or a composition comprising the extract, prior to exposure of the cell to the filovirus, wherein the exposing the cell to the Melissa officinalis extract, or a composition comprising Melissa officinalis extract inhibits filovirus infection.

4. A method of treating a subject infected with filovirus, the method comprising administering an effective amount of an extract from Melissa officinalis , or a composition comprising the extract, to the subject, thereby treating the subject infected with filovirus.

5. A method of preventing infection of a subject with filovirus, the method comprising administering an effective amount of an extract from Melissa officinalis , or a composition comprising the extract, to the subject prior to exposure of the subject to filovirus, thereby preventing infection with filovirus.

6. The method of claim 4 , wherein the filovirus is an Ebola virus.

7. The method of claim 4 , wherein the filovirus is Marburg virus.

8. The method of claim 5 , wherein the filovirus is an Ebola virus.

9. The method of claim 5 , wherein the filovirus is Marburg virus.

10. The method of claim 1 , wherein the cell is a human cell.

11. The method of claim 1 , wherein the filovirus is an Ebola virus.

12. The method of claim 1 , wherein the filovirus is Marburg virus.

13. The method of claim 1 , wherein the extract comprises dried plant material and extraction solution.

14. The method of claim 1 , wherein the extract comprises fresh plant material and extraction solution.

15. The method of claim 13 , wherein the extraction solution comprises water.

16. The method of claim 15 , wherein the extraction solution further comprises glycerin.

17. The method of claim 16 , wherein the extraction solution comprises glycerin in an amount ranging from about 0.01% to about 90%.

18. The method of claim 15 , wherein the extraction solution further comprises ethanol.

19. The method of claim 18 , wherein the extraction solution comprises ethanol in an amount ranging from about 0.01% to about 30%.

Assignees

• Langland Jeffrey
• Jacobs Bertram
• Denzler Karen
• Univ Arizona State

Inventors

• Langland Jeffrey
• Jacobs Bertram
• Denzler Karen

Classifications

• A61K36/53 Primary

  Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender · CPC title

• A61K2236/333

  using mixed solvents, e.g. 70% EtOH · CPC title

• A61P31/14

  for RNA viruses · CPC title