Analysis method and analysis kit for simultaneously detecting or quantitating multiple types of target substances

Patent metadata

Field	Value
Publication number	US-10288606-B2
Application number	US-201314437450-A
Country	US
Kind code	B2
Filing date	Oct 21, 2013
Priority date	Oct 22, 2012
Publication date	May 14, 2019
Grant date	May 14, 2019

Abstract

The present invention provides an analysis method for simultaneously detecting or quantifying plural species of target substances by measuring the light emission from plural carriers within a single container from outside of the container, these carriers comprising a plurality of substances that are respectively immobilized thereon for capturing the plural species of target substances, wherein the light emission from a desired carrier is selectively received by measuring the emission from the carriers through an optical filter. The present invention also provides an analysis kit for simultaneously detecting or quantifying plural species of target substances by measuring the light emission from plural carriers within a single container from outside of the container, these carriers comprising a plurality of substances that are respectively immobilized thereon for capturing the plural species of target substances, which comprises the plural carriers and an optical filter for selectively receiving the emission from a desired carrier.

First claim

The invention claimed is:

1. An analysis method for simultaneously detecting or quantifying plural species of target substances comprising:
measuring a light emission from a plurality of carriers each with a respective target substance immobilized thereon within a single tube or pipette shaped container from outside of the container to detect or quantify the plural species of target substances immobilized on the carriers,
wherein the light emission from a desired carrier is selectively received by measuring the emission from the carrier through an optical filter made of the container itself, and
wherein the optical filter is configured to suppress interference with the light emission from non-desired carriers.

2. The method according to claim 1 , wherein the optical filter has a light transmittance of 70% or less in the wavelength band of emission from the carrier.

3. The method according to claim 1 , wherein the carriers are spherical.

4. The method according to claim 1 , wherein the carriers are arranged one-dimensionally or two-dimensionally.

5. The method according to claim 1 , wherein the emission is chemiluminescence or fluorescence and has a wavelength band within the range of 340-900 nm.

6. The method according to claim 2 , wherein the carriers are spherical.

7. The method according to claim 2 , wherein the carriers are arranged one-dimensionally or two-dimensionally.

8. The method according to claim 3 , wherein the carriers are arranged one-dimensionally or two-dimensionally.

Assignees

• Universal Bio Research Co Ltd

Inventors

• Sugimoto Takaki
• Ueda Tetsuya
• Kobayashi Shuichi
• Tajima Hideji

Classifications

• G01N2021/7793

  Sensor comprising plural indicators · CPC title

• G01N21/6428 Primary

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• G01N2333/59

  Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH] · CPC title

• G01N33/54306 Primary

  Solid-phase reaction mechanisms · CPC title

• G01N33/54313

  the carrier being characterised by its particulate form · CPC title