Structuring fats and methods of producing structuring fats

Patent metadata

Field	Value
Publication number	US-10287613-B2
Application number	US-201815875984-A
Country	US
Kind code	B2
Filing date	Jan 19, 2018
Priority date	Apr 18, 2012
Publication date	May 14, 2019
Grant date	May 14, 2019

Abstract

Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

First claim

What is claimed is:

1. A method of producing a triglyceride oil, the method comprising:
cultivating a recombinant microalgal cell expressing an exogenous polynucleotide encoding a ketoacyl-ACP synthase; and
extracting the oil produced by the cell;
wherein the triglyceride oil is enriched in stearic-oleic-stearic triglyceride as compared to a non-recombinant microalgal cell.

2. The method of claim 1 , wherein the microalgal cell is a cell of the genus Prototheca or Chlorella.

3. The method of claim 2 , wherein the microalgal cell is a Prototheca moriformis cell.

4. The method of claim 1 , wherein the recombinant microalgal cell further comprises an exogenous polynucleotide that encodes a fatty acyl-ACP thioesterase.

5. The method claim 1 , wherein the recombinant microalgal cell further comprises nucleic acids operable to reduce the expression of stearoyl-ACP desaturase or a fatty acyl desaturase.

6. The method of claim 5 , wherein the nucleic acids operable to reduce the expression of a stearoyl-ACP desaturase or a fatty acyl desaturase is a nucleic acid that encodes an RNA construct that reduces the activity of stearoyl-ACP desaturase or the fatty acyl desaturase.

7. The method of claim 1 , wherein the oil is enriched in triglycerides of the saturated-unsaturated-saturated type, wherein the sum of palmitic-oleic-palmitic, stearic-oleic-stearic and palmitic-oleic-stearic is at least 30% of the triglycerides.

8. The method of claim 7 , wherein the sum of palmitic-oleic-palmitic, stearic-oleic-stearic and palmitic-oleic-stearic is at least 50% of the triglycerides.

9. The method of claim 8 , wherein the oil comprises triglycerides comprising at least 50% stearic-oleic-stearic, and optionally less than 10% stearic-stearic-stearic.

10. The method of claim 1 , wherein the recombinant microalgal cell further comprises an exogenous sucrose invertase gene so that the cell can grow on sucrose as a sole carbon source.

11. A method of producing a triglyceride oil, the method comprising:
cultivating a recombinant microalgal cell expressing an exogenous polynucleotide encoding a ketoacyl-ACP synthase and a fatty acyl-ACP thioesterase; and
extracting the oil produced by the cell;
wherein the triglyceride oil is enriched in stearic-oleic-stearic triglyceride as compared to a non-recombinant microalgal cell.

12. The method of claim 11 , wherein the microalgal cell is a cell of the genus Prototheca or Chlorella.

13. The method of claim 12 , wherein the microalgal cell is a cell of the genus Prototheca.

14. The method of claim 13 , wherein the microalgal cell is a Prototheca moriformis cell.

15. The method claim 11 , wherein the recombinant microalgal cell further comprises nucleic acids operable to reduce the expression of stearoyl-ACP desaturase or a fatty acyl desaturase.

16. The method of claim 11 , wherein the nucleic acids operable to reduce the expression of a stearoyl-ACP desaturase or a fatty acyl desaturase is a nucleic acid that encodes an RNA construct that reduces the activity of stearoyl-ACP desaturase or the fatty acyl desaturase.

17. The method of claim 11 , wherein the oil is enriched in triglycerides of the saturated-unsaturated-saturated type, wherein the sum of palmitic-oleic-palmitic, stearic-oleic-stearic and palmitic-oleic-stearic is at least 30% of the triglycerides.

18. The method of claim 17 , wherein the sum of palmitic-oleic-palmitic, stearic-oleic-stearic and palmitic-oleic-stearic is at least 50% of the triglycerides.

19. The method of claim 18 , wherein the oil comprises triglycerides comprising at least 50% stearic-oleic-stearic, and optionally less than 10% stearic-stearic-stearic.

20. The method of claim 11 , wherein the recombinant microalgal cell further comprises an exogenous sucrose invertase gene so that the cell can grow on sucrose as a sole carbon source.

Assignees

• Corbion Biotech Inc

Inventors

• Franklin Scott
• Somanchi Aravind
• Rudenko George N
• Bhat Riyaz
• Zhao Xinhua
• Bond Risha
• Rakitsky Walter
• Marangoni Alejandro
• Braksmayer Diza

Classifications

• C12Y114/19006

  DELTA12-fatty-acid desaturase (1.14.19.6), i.e. oleoyl-CoA DELTA12 desaturase · CPC title

• C12P7/6409 Primary

  Fatty acids · CPC title

• C12Y302/01026

  Beta-fructofuranosidase (3.2.1.26), i.e. invertase · CPC title

• C12N9/1029

  transferring groups other than amino-acyl groups (2.3.1) · CPC title

• C12Y203/0102

  Diacylglycerol O-acyltransferase (2.3.1.20) · CPC title