In vitro method for culturing stem cells

Patent metadata

Field	Value
Publication number	US-10287553-B2
Application number	US-201314395811-A
Country	US
Kind code	B2
Filing date	Apr 22, 2013
Priority date	Apr 20, 2012
Publication date	May 14, 2019
Grant date	May 14, 2019

Abstract

There is provided a method for culturing a stem cell in vitro. The method comprises providing a substrate surface coated with a coating comprising a molecule having a catechol moiety or a polymer thereof; and growing a stem cell on said coated substrate surface in a growth medium.

First claim

What is claimed is:

1. An in vitro method for expanding a pluripotent stem cell by self-renewal while maintaining the level of differentiation of the stem cell, the method comprising:
providing a substrate surface coated with a coating comprising L-3,4-dihydroxy-phenylalanine (L-DOPA); and
growing a pluripotent stem cell attached on said coated substrate surface in a growth medium under growth conditions that do not induce differentiation of the stem cell for at least 10 passages.

2. The method according to claim 1 , wherein the pluripotent stem cell is a human pluripotent stem cell.

3. The method according to claim 1 , wherein the pluripotent stem cell is a non-human animal pluripotent stem cell.

4. The method according to claim 1 , wherein the L-DOPA comprises one or both of an L-DOPA monomer and an L-DOPA polymer.

5. The method according to claim 1 , wherein the coating further comprises one or more of a protein, a peptide, a polysaccharide, a growth factor and a hormone.

6. The method according to claim 5 , wherein the protein, the peptide, the polysaccharide, the growth factor or the hormone is a synthetic or recombinant molecule.

7. The method according to claim 1 , wherein the growth medium:
(a) is free from non-human serum;
(b) is free from non-human animal components; or
(c) comprises one or more components derived from non-human animal blood, cells or tissue.

8. The method according to claim 7 , wherein the growth medium comprises non-human serum.

9. The method according to claim 1 , wherein the growth medium:
(a) is free from human serum;
(b) is free from human components; or
(c) comprises one or more components derived from human blood, cells or tissue.

10. The method according to claim 1 , wherein the growth medium comprises human serum.

11. The method according to claim 1 , wherein the growth medium is synthetic and is free from animal components, including human and non-human animal components.

12. The method according to claim 1 , wherein the growth medium comprises one or more recombinant or synthetic proteins or peptides.

13. The method according to claim 1 , wherein the pluripotent stem cell is an embryonic stem cell or an induced pluripotent stem cell.

14. The method according to claim 1 , wherein said growing comprises adding Rho-associated kinase (ROCK) inhibitor to the growth medium.

Assignees

• Agency Science Tech & Res

Inventors

• Zink Daniele
• Ni Ming
• Narayanan Karthikeyan
• Kandasamy Karthikeyan
• Wan Andrew C A
• Ying Jackie Y

Classifications

• C12N2533/20

  Small organic molecules · CPC title

• C12N2533/30

  Synthetic polymers · CPC title

• C12N5/0663

  Bone marrow mesenchymal stem cells (BM-MSC) · CPC title

• C12N5/0075

  using microcarriers · CPC title

• C12N5/0696 Primary

  Artificially induced pluripotent stem cells, e.g. iPS · CPC title