Process and device for isolating cells from biological tissue

Patent metadata

Field	Value
Publication number	US-10287543-B2
Application number	US-201615333337-A
Country	US
Kind code	B2
Filing date	Oct 25, 2016
Priority date	Nov 19, 2015
Publication date	May 14, 2019
Grant date	May 14, 2019

Abstract

Use of the perfusion device in a process for disaggregation of a biological tissue to yield target cells.

First claim

What is claimed is:

1. A perfusion device for biological tissues, comprising:
a casing having two parts, a first part ( 1 ) and a second part ( 9 );
a holder ( 7 ) for a plurality of hollow penetration structures ( 8 ), wherein the hollow penetration structures ( 8 ) are provided with at least one orifice having fluid communication through the holder ( 7 );
and
a support ( 5 ) for the biological tissue ( 6 )

characterized in that the support ( 5 ) for the biological tissue ( 6 ) is positioned in the casing at a distance to the holder ( 7 ) that by joining the first part ( 1 ) and the second part ( 9 ) to form the casing, the hollow penetration structures ( 8 ) are in proximity to the holder ( 7 )) wherein the first part ( 1 ) and the second part have threads disposed thereon, and are opened and closed in a watertight matter using threads disposed on the first part ( 1 ) and the second part ( 2 ), and wherein the holder for the plurality of hollow penetration structures ( 8 ) and the support, are single-use, plastic disposables.

2. The perfusion device according to claim 1 , characterized in that by joining the first part ( 1 ) and the second part ( 9 ) to form the casing, the hollow penetration structures ( 8 ) penetrate at least in part into the biological tissue ( 6 ).

3. The perfusion device according to claim 1 , characterized in that the first part ( 1 ) and the second part ( 9 ) of the casing are each provided with a corresponding closing mechanism ( 11 , 12 ) for joining the two parts.

4. The perfusion device according to claim 1 , characterized in that the hollow penetration structures ( 8 ) extend 1 to 100 mm from the holder ( 7 ).

5. The perfusion device according to claim 1 , characterized in that the hollow penetration structures ( 8 ) are elongated objects having a needle-, cone- or pyramid-like shape.

6. The perfusion device according to claim 1 , characterized in that the support ( 5 ) is a filter, mesh, rack, grid or a plate with orifices.

7. The perfusion device according to claim 1 , characterized in that the holder ( 7 ) can be inserted and removed from the casing.

8. The perfusion device according to claim 1 , characterized in that the first part ( 1 ) and the second part ( 9 ) of the casing are provided with a storage cavity ( 14 ) for fluids and excess reagent, and further comprising a pump that recirculates the excess reagent and administers it again to the biological sample.

9. The perfusion device according to claim 8 , wherein the excess reagent is a disaggregating agent.

10. A process for disaggregation of a biological tissue comprising:
providing the perfusion device of claim 1 ;
penetrating the biological tissue by at least one of the hollow penetration structures ( 8 ) by joining the first part ( 1 ) and the second part ( 9 ) to form the casing with the threads; and
administering the at least one agent through the hollow penetration structures ( 8 ) into the biological tissue ( 6 ).

11. The process according to claim 10 , wherein the biological tissue ( 6 ) is first washed with buffer to remove non-target cells from the biological tissue ( 6 ).

12. The process according to claim 10 , wherein the agent to disaggregate the biological tissue ( 6 ) and/or the buffer is repeatedly administered into the biological tissue ( 6 ).

13. The process according to claim 10 , wherein the agent to disaggregate the biological tissue ( 6 ) is selected from the group consisting of trypsin, chymotrypsin, papain, collagenase, elastase, dispase, thermolysin, hyaluronidase, clostripain and neutral protease from clostridium histolyticum , pronase, DNase I, pepsin, proteinase K, lysozyme, chelating agents for bivalent ions and mixtures thereof.

14. The process according to claim 10 , wherein the target cells are tissue-resident cells.

15. The process according to claim 10 , wherein the biological tissue is selected from the group consisting of spleen, heart, liver, brain and other neural tissues, kidney, lung, pancreas, breast, umbilical cord, skin, placenta, ovary, oviduct, uterus, prostate, tonsil, thymus, stomach, testis, trachea, cartilage, tendon, bone, skeletal muscle, smooth muscle, gut, colon, intestine, bladder, urethra, eye, gall bladder, organoids from cell cultures and tumors.

Assignees

• Miltenyi Biotec Gmbh

Inventors

• Poggel Carsten
• Bosio Andreas
• Stoters Wolfgang
• Adams Timo

Classifications

• G01N1/286 Primary

  involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F) · CPC title

• C12M23/28

  disposable or single use · CPC title

• C12M21/18

  Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes · CPC title

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• C12N5/0081

  Purging biological preparations of unwanted cells · CPC title