Methods and articles for detecting deoxyribonuclease activity

Patent metadata

Field	Value
Publication number	US-10280446-B2
Application number	US-201715651164-A
Country	US
Kind code	B2
Filing date	Jul 17, 2017
Priority date	Feb 26, 2009
Publication date	May 7, 2019
Grant date	May 7, 2019

Abstract

The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms.

First claim

What is claimed is:

1. A detection article, comprising:
a body member comprising a self-supporting, waterproof substrate having upper and lower surfaces;
wherein a dry coating comprising a cold water-soluble gelling agent and an indicator system is adhered uniformly onto a portion of at least one surface of the body member;
wherein the indicator system comprises DNA and methyl green; and
wherein, after contact with a predetermined volume of an aqueous liquid, the gelling agent forms a hydrogel and the concentration of methyl green in the hydrogel is about 5 μg/mL to about 20 μg/mL.

2. The article of claim 1 , wherein the coating further comprises a nutrient medium, an indicator, a selective agent, or any combination of two or more of the foregoing.

3. The article of claim 2 , wherein the selective agent selects for the growth of Staphylococcus aureus.

4. The article of claim 3 , wherein the selective agent is selected from the group consisting of cephalothin, cefazolin, cephradine, cephalexin, cefadroxil, cefamandole, cefoxitin, cefaclor, cefuroxime, cefuroxime axetil, loracarbef, cefonicid cefotetan, ceforanide, cefotaxime, cefpodoxime proxetil, ceftizoxime, cefixmeceftriaxone, cefoperazone, ceftazidime, moxlactam, cefepime, cefpirome, and oxacillin.

Assignees

• 3M Innovative Properties Co

Inventors

• Mach Patrick A
• Rosauer Michelle L
• Hughes Michael E

Classifications

• G01N2333/922

  Ribonucleases (RNAses); Deoxyribonucleases (DNAses) · CPC title

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• C12Q1/34 Primary

  involving hydrolase · CPC title

• C12N11/04

  entrapped within the carrier, e.g. gel or hollow fibres · CPC title

• G01N2333/31

  from Staphylococcus (G) · CPC title