Glycosylated repeat-motif-molecule conjugates

Patent metadata

Field	Value
Publication number	US-10280214-B2
Application number	US-201213464878-A
Country	US
Kind code	B2
Filing date	May 4, 2012
Priority date	Nov 5, 2009
Publication date	May 7, 2019
Grant date	May 7, 2019

Abstract

Herein are reported glycosylated repeat-motif-molecule conjugate of the following formula: (repeat-motif-molecule−linker n ) m −conjugation partner−(linker o −repeat-motif-molecule) p , wherein n and o are independently of each other and independently for each value of m and p integer values of 0 or 1, and m and p are independently of each other integer values of 0 or 1 or 2 or 3 or 4 or 5 or 6 or 7, and wherein the repeat-motif-molecule conjugate comprises at least one oligosaccharide attached to a glycosylation site. Also reported are encoding nucleic acids and method for producing these repeat-motif-conjugates in mammalian cells.

First claim

What is claimed is:

1. Glycosylated ankyrin repeat-motif-molecule conjugate of the following formula
(ankyrin repeat-motif-molecule−linker n ) m −(conjugation partner) q −(linker o −ankyrin repeat- motif-molecule) p

wherein n and o are independent of each other and independent for each value of m and p for integer values of 0 or 1;
wherein m is 1, p is 0 and q is 1, or m is 0, p is 1 and q is 1;
wherein the ankyrin repeat-motif-molecule conjugate comprises at least one oligosaccharide attached to a glycosylation site; and
wherein the conjugation partner comprises an engineered pair of polypeptide chains comprising a human IgG1 heavy chain hinge region and human IgG1 CH2 and CH3 domains, wherein the conjugation partner comprises two separate polypeptide chains linked by three disulfide bonds in the hinge region, and wherein the conjugation partner does not contain a variable domain.

2. Conjugate according to claim 1 , characterized in that said ankyrin-repeat-motif-molecule comprises the amino acid sequence of SEQ ID NO: 4 or a variant thereof.

3. Conjugate according to claim 1 , characterized in that said linker is a peptidic linker selected from SEQ ID NO: 14 and SEQ ID NO: 25 to SEQ ID NO: 33.

4. Conjugate according to claim 1 , characterized in that said conjugation partner is a multimerizing conjugation partner.

5. Conjugate according to claim 1 , characterized in that
i) n=1, m=1, q=1, p=0, or
ii) n=0, m=1, q=1, p=0.

6. Conjugate according to claim 1 , characterized in that
i) m=0, q=1, p=1, o=0, or
ii) m=0, q=1, p=1, o=1.

7. Conjugate according to claim 1 , characterized in that said conjugation partner comprises the amino acid residues 121 to 352 of SEQ ID NO:11.

8. Conjugate according to claim 7 , characterized in that said linker is a peptidic linker selected from SEQ ID NO: 18 to SEQ ID NO: 24.

Assignees

• Fischer Stephan
• Imhof Jung Sabine
• Kopetzki Erhard
• Hoffmann La Roche

Inventors

• Fischer Stephan
• Imhof-Jung Sabine
• Kopetzki Erhard

Classifications

• C07K2319/30

  Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto · CPC title

• C07K2317/52

  Constant or Fc region; Isotype · CPC title

• C07K16/32

  against translation products of oncogenes · CPC title

• C07K2318/20

  Antigen-binding scaffold molecules wherein the scaffold is not an immunoglobulin variable region or antibody mimetics · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title