Method of detecting cancer stem cell, method of screening cancer stem cell, and method of inhibiting cancer stem cell

Patent metadata

Field	Value
Publication number	US-10278965-B2
Application number	US-201715678596-A
Country	US
Kind code	B2
Filing date	Aug 16, 2017
Priority date	Oct 26, 2012
Publication date	May 7, 2019
Grant date	May 7, 2019

Abstract

An object of the present invention is to provide a cancer cell inhibitory drug, particularly a cancer stem-cell inhibitory drug, or a cancer stem-cell detection probe. The present invention provides a cancer cell inhibitory drug comprising at least one compound represented by general formula (1) as an active ingredient

First claim

The invention claimed is:

1. A method of inhibiting growth of any one of a pancreatic cancer cell, a prostatic cancer cell, and a cancer stem cell of chronic myelocytic leukemia in a subject in need thereof, comprising administering to the subject a composition comprising, as an active ingredient, any one of compounds (2) to (13) and (15) to (60).

2. The method according to claim 1 , wherein the subject is a human.

3. A method of detecting a composition taken into a cancer cell selected from the group consisting of a pancreatic cancer cell, a prostatic cancer cell, and a cancer stem cell of chronic myelocytic leukemia in a subject in need thereof, comprising:
administering to the subject a composition comprising, as an active ingredient, a compound selected from the group consisting of compounds (2) to (13) and (15) to (60); and
detecting the composition taken into the cancer cell:

4. A method of screening a cancer cell selected from the group consisting of a pancreatic cancer cell, a prostatic cancer cell, and a cancer stem cell of chronic myelocytic leukemia in a subject in need thereof, comprising:
administering to the subject a composition comprising, as an active ingredient, any one of compounds (2) to (13) and (15) to (60); and
detecting the cancer cell selectively:

5. The method according to claim 3 , further comprising detecting fluorescent intensity derived from the compound.

6. The method according to claim 3 , wherein the subject is a transplanted model animal.

7. The method according to claim 3 , wherein the detecting of the composition taken into the cancer stem cell is done in vivo.

8. The method according to claim 3 , further comprising applying excitation light to the subject and obtaining a fluorescent image of the subject.

9. The method according to claim 3 , further comprising detecting light intensity derived from the compound.

10. The method according to claim 1 , wherein the subject is a vertebrate animal, an amphibian, or a mammal.

11. The method according to claim 3 , wherein the subject is a vertebrate animal, an amphibian, or a mammal.

12. The method according to claim 4 , wherein the subject is a vertebrate animal, an amphibian, or a mammal.

Assignees

• Canon Kk

Inventors

• Shintou Taichi
• Nomoto Tsuyoshi
• Watanabe Kohei
• Miyazaki Takeshi
• Tanaka Toshio
• Shimada Yasuhito
• Nishimura Yuhei

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• C09B23/0025

  the substituent being bound through an oxygen atom · CPC title

• C09B23/083

  five >CH- groups · CPC title

• A61K49/0032

  Methine dyes, e.g. cyanine dyes · CPC title