Encoded microcapsules and microarray fabricated therefrom

Patent metadata

Field	Value
Publication number	US-10274504-B2
Application number	US-201314064217-A
Country	US
Kind code	B2
Filing date	Oct 28, 2013
Priority date	Oct 28, 2013
Publication date	Apr 30, 2019
Grant date	Apr 30, 2019

Abstract

Disclosed is a microcapsule encoded depending on the kind of a target substance included therein. The encoded microcapsule has a hydrophilic liquid core including the target substance and a hydrophobic shell surrounding the liquid core. The encoded microcapsule includes graphical codes introduced on the surface of the shell.

First claim

What is claimed is:

1. Microcapsules encoded depending on a kind of a target substance present therein, comprising:
a hydrophilic liquid core comprising the target substance;
a hydrophobic shell surrounding the liquid core, the hydrophobic shell comprising a photoinitiator,
wherein the photoinitiator has a chromophore capable of emitting fluorescence after decomposition of the photoinitiator; and
graphical codes engraved on a surface of the hydrophobic shell so that the microcapsules are encoded in order to distinguish from each other depending on the kind of the target substance being included in the hydrophilic liquid core
wherein each of the graphical codes emits florescent light at a higher intensity than the rest of the surface of the hydrophobic shell.

2. The encoded microcapsules according to claim 1 , wherein the target substance is a chemical substance or a biological substance.

3. The encoded microcapsules according to claim 2 , wherein the chemical substance is a drug or the biological substance is selected from the group consisting of cells, molecules, proteins, bacteria, DNAs, RNAs, and combinations thereof.

4. The encoded microcapsules according to claim 1 , wherein the liquid core is a water droplet.

5. The encoded microcapsules according to claim 1 , wherein the shell is formed by curing a photocurable polymer in the presence of the photoinitiator.

6. The encoded microcapsules according to claim 1 , wherein the graphical codes contain a fluorescent material derived from the photoinitiator.

7. A method of producing encoded microcapsules, the method comprising:
preparing double emulsion droplets, each of which has a hydrophilic liquid core comprising a target substance and a hydrophobic shell surrounding the liquid core, wherein the hydrophobic shell contains a photocurable polymer and a photoinitiator having a chromophore;
by an external energy source, curing the photocurable polymer to form core-shell structured microcapsules; and
engraving graphical codes on a surface of the hydrophobic shell by irradiating patterned energy on the cured core shell microcapsules and distinguishing among individual microcapsules by fluorescence emitted from the chromophore of the photoinitiator in the engraved region of the surface,
wherein the photoinitiator includes a moiety capable of being decomposed by an external energy source and the chromophore is capable of emitting the fluorescence after decomposition of the photoinitiator.

8. The method according to claim 7 , wherein the photoinitiator is 2,2-dimethoxy-2-phenylacetophenone.

9. The method according to claim 7 , wherein the double emulsion droplets are prepared using a microfluidic chip comprising a channel area having a hydrophobic surface and a channel area having a hydrophilic surface.

10. The method according to claim 7 , wherein the patterned energy is irradiated through a digital micromirror device (DMD).

11. The method according to claim 7 , wherein the patterned energy is irradiated by lithography using a mask.

12. The method according to claim 11 , wherein the patterned energy is irradiated in a bi-directional encoding manner using two masks interposing the microcapsules therebetween.

13. The method according to claim 11 , wherein the patterned energy is irradiated in such a manner that the mask and a planar substrate are arranged to face each other through the microcapsules, pressed to each other, followed by bi-directional encoding.

14. An assay method using microcapsules encoded so as to be distinguished from each other depending on the kind of target substances present therein, the method comprising:
introducing the encoded microcapsules of claim 1 into a substrate of microwells; reading the codes of the encoded microcapsules arranged inside the microwells; introducing analytes into the microwells; and
breaking the microcapsules introduced into the microwells to allow the target substances to react with the analytes.

15. The assay method according to claim 14 , further comprising forming layers of a liquid immiscible with the liquids inside the microcapsules introduced into the microwells, on the microwells such that the microwells are distinguished from each other as independent reaction spaces.

16. The assay method according to claim 14 , wherein the microcapsules are broken in such a manner that the substrate is bonded and pressed to an extra substrate with micropillars disposed corresponding to the microwells.

17. A microarray, comprising:
microcapsules encoded to be distinguished from each other depending on a kind of target substances;
a substrate having microwells, wherein said microcapsules are introduced in the substrate; and
core liquids having the target substances being released when the microcapsules are broken,
wherein each of the microcapsules, comprises;
a hydrophilic liquid core comprising the target substances,
a hydrophobic shell surrounding the liquid core, the hydrophobic shell comprising a photoinitiator,
wherein the photoinitiator has a chromophore capable of emitting fluorescence after decomposition of the photoinitiator, and
graphical codes engraved on a surface of the hydrophobic shell so that the microcapsules are encoded in order to distinguish from each other depending on the kind of the target substance being included in the hydrophilic liquid core
wherein each of the graphical codes emits florescent light at a higher intensity than the rest of the surface of the hydrophobic shell.

Assignees

• Snu R&Db Foundation
• Quantamatrix Inc

Inventors

• Kwon Sunghoon
• Song Younghoon
• Kwon Taehong
• Lee Daewon
• Kim Junhoi

Classifications

• G01N33/94 Primary

  involving narcotics {or drugs or pharmaceuticals, neurotransmitters or associated receptors} · CPC title

• G01N33/5432

  Liposomes or microcapsules · CPC title