Particle processing systems and methods for normalization/calibration of same

Patent metadata

Field	Value
Publication number	US-10274414-B2
Application number	US-201414281353-A
Country	US
Kind code	B2
Filing date	May 19, 2014
Priority date	Feb 5, 2010
Publication date	Apr 30, 2019
Grant date	Apr 30, 2019

Abstract

Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.

First claim

What is claimed:

1. A computer-implemented method for controlling a particle processing system having a particle inspection system to reduce variability using processed flow cytometry data, the method comprising:
receiving, by a processor, a baseline value for a population's first characteristic for a first population of particles, the baseline value generated from an analysis of a first set of data associated with the first population of particles flowing through a first flow path during a first particle processing operation, wherein the first population of particles is associated with a first particle type;
processing, using the processor, a second set of data associated with a second population of particles flowing through a second flow path different than the first flow path during a second particle processing operation to generate a plurality of individual particle values for the first characteristic, the second population of particles being of the same particle type as the first particle type;
generating, using the processor, adjustment factors based on the population's baseline value for the first characteristic of the first population of particles and the individual particle values for the first characteristic associated with the second population of particles;
applying, using the processor, the adjustment factors to detected output values representative of a second particle characteristic associated with the second population of particles during the second particle processing operation, the second particle characteristic being different from the first characteristic, to generate an adjusted second set of particle data; and
controlling one or more operational parameters of the particle inspection system relative to the second flow path based on the adjusted second set of particle data.

2. The method of claim 1 , wherein the first set of data is associated with a first particle processing system and the second set of data is associated with a second particle processing system different than the first particle processing system.

3. The method of claim 2 , wherein the second particle processing system is located at a geographic location different from a geographic location of the first particle processing system.

4. The method of claim 1 , wherein the first set of data is associated with a first particle inspection system and the second set of data is associated with a second particle inspection system different than the first particle inspection system.

5. The method of claim 1 , further comprising:
collectively analyzing the first set of data and the adjusted second set of particle data to develop common sorting criteria for a particle sorting operation.

6. A particle analyzing system comprising:
at least one processor configured to perform the method according to claim 1 .

7. The method of claim 1 , wherein the population's first characteristic is an average or a common velocity of the first population of particles flowing through the first flow path, and wherein the individual particle's first characteristic is a particle velocity.

8. The method of claim 1 , wherein the second particle characteristic is a particle pulse area.

9. The method of claim 1 , wherein the second particle characteristic is a particle pulse height.

10. The method of claim 1 , wherein the second particle characteristic is a particle fluorescence.

11. The method of claim 1 , wherein the second particle characteristic is a particle light extinction.

12. The method of claim 1 , further comprising:
determining values of the second particle characteristic for the first set of particles flowing through the first flow path;
visually displaying the values of the second particle characteristic for the first set of particles; and
visually superimposing the adjusted particle data for the second particle characteristic associated with the second set of particles flowing through the second flow path onto the visually displayed values of the second particle characteristic for the first set of particles flowing through the first flow path.

13. A method for controlling a particle processing system having a particle inspection system to reduce variability using adjusted flow cytometry data, the method comprising:
determining, during a particle processing operation, an average or a common velocity of a first set of particles flowing through a first flow path, wherein the first set of particles are associated with a first particle type;
determining, during the same particle processing operation, particle velocities for a second set of particles flowing through a second flow path different than the first flow path, wherein the second set of particles are the same particle type as the first particle type;
generating, during the same particle processing operation, adjustment factors based on the average or the common velocity of the first set of particles and the particle velocities of the second set of particles;
applying, during the same particle processing operation, the adjustment factors to measurement values for a particle characteristic associated with the second set of particles, the particle characteristic being other than velocity, to generate adjusted particle data; and
controlling one or more operational parameters of the particle inspection system relative to the second flow path based on the adjusted particle data.

14. The method of claim 13 , further comprising:
determining measurement values of the particle characteristic of the first set of particles flowing through the first flow path;
visually displaying the measurement values of the first set of particles; and
visually superimposing the adjusted particle data from the second set of particles flowing through the second flow path onto the visually displayed measurement values from the first set of particles flowing through the first flow path.

15. The method of claim 13 , wherein the first and second flow paths are provided on a single microfluidic chip.

Assignees

• Cytonome St Llc

Inventors

• Sharpe Johnathan Charles
• Machado Emanuel Tito Mendes
• Hulspas Rudolf

Classifications

• G01N15/1012

  Calibrating particle analysers; References therefor · CPC title

• G01N2015/1402

  Data analysis by thresholding or gating operations performed on the acquired signals or stored data · CPC title

• G01N15/1484 Primary

  microstructural devices · CPC title

• G01N21/53

  within a flowing fluid, e.g. smoke · CPC title

• G01N15/1429

  Signal processing · CPC title