Emulsion-based fermentation for accelerated gas substrate mass transfer

Patent metadata

Field	Value
Publication number	US-10273510-B2
Application number	US-201715418337-A
Country	US
Kind code	B2
Filing date	Jan 27, 2017
Priority date	Jan 29, 2016
Publication date	Apr 30, 2019
Grant date	Apr 30, 2019

Abstract

Described here is a method for increasing the transfer of a gas substrate in microbial fermentation, comprising incubating an emulsion comprising an oil phase and an aqueous phase droplet dispersed in the oil phase, and supplying the gas substrate to the oil phase, wherein the aqueous phase droplet comprises a microorganism, and wherein the emulsion is stabilized by a surfactant or an amphiphilic particle that is adsorbed to an interface of the oil phase and the aqueous phase. Also described is an emulsion for microbial fermentation, comprises an oil phase and an aqueous phase droplet dispersed in the oil phase, wherein the aqueous phase droplet comprises a microorganism, wherein the emulsion comprises a gas substrate externally-supplied to the oil phase, and wherein the emulsion is stabilized by a surfactant or an amphiphilic particle that is adsorbed to an interface of the oil phase and the aqueous phase.

First claim

What is claimed is:

1. A method for increasing the transfer of a gas substrate in microbial fermentation, comprising incubating an emulsion comprising an oil phase and an aqueous phase droplet dispersed in the oil phase, and supplying the gas substrate to the oil phase, wherein the aqueous phase droplet comprises a microorganism, and wherein the emulsion is stabilized by a surfactant or an amphiphilic particle that is adsorbed to an interface of the oil phase and the aqueous phase droplet.

2. The method of claim 1 , wherein the aqueous phase droplet comprises a microorganism selected from the group consisting of carbon monoxide-utilizers, hydrogen utilizers, and alkane utilizers.

3. The method of claim 1 , wherein the gas substrate is selected from the group consisting of carbon monoxide, hydrogen, and alkane.

4. The method of claim 1 , wherein the aqueous phase droplet comprises a methanotroph, and wherein the gas substrate is methane.

5. The method of claim 4 , wherein the methanotroph is adapted to produce a bioproduct of biodiesel generation, propylene oxide production, single cell protein production, extracellular polysaccharides production, or human health supplements production.

6. The method of claim 4 , wherein the methanotroph is adapted to produce a polyhydroxyalkanoate, and the method further comprises recovering the polyhydroxyalkanoate produced.

7. The method of claim 1 , wherein the oil phase comprises a fluid that has a higher solubility for the gas substrate than the aqueous phase and is capable of generating a stable emulsion with the aqueous phase.

8. The method of claim 1 , wherein the oil phase comprises a hydrocarbon.

9. The method of claim 1 , wherein the oil phase comprises a fluorocarbon.

10. The method of claim 1 , wherein the oil phase comprises at least one of C 9 H 50 F 15 (HFE-7500), C 21 F 48 N 2 (FC-40), or perfluoromethyldecalin (PFMD).

11. The method of claim 1 , wherein the emulsion is stabilized by a fluoro-surfactant comprising a poly(ethylene glycol)-perfluorinated polyether (PEG-PFPE) amphiphilic block copolymer.

12. The method of claim 1 , wherein the emulsion is stabilized by a partially fluorinated silica nanoparticle.

13. The method of claim 12 , wherein the aqueous phase droplet further comprises a hydrophilic polymer adsorbed to the partially fluorinated silica nanoparticle at the interface.

14. The method of claim 12 , wherein the partially fluorinated silica nanoparticle is covalently grafted with a hydrophilic polymer.

15. The method of claim 1 , wherein the method is substantially free of mechanical stirring or agitation of the emulsion during incubation.

16. The method of claim 1 , wherein the aqueous phase droplet has a dimension greater than or equal to 50 nm and less than or equal to 1000 microns.

Assignees

• Univ Leland Stanford Junior

Inventors

• Tang Sindy
• Criddle Craig
• Myung Jaewook
• Kim Minkyu

Classifications

• C12N1/26

  Processes using, or culture media containing, hydrocarbons (refining of hydrocarbon oils by using microorganisms C10G32/00) · CPC title

• Y02E50/13

  Cross-Sectional Technologies · mapped topic

• C12P7/625 Primary

  Polyesters of hydroxy carboxylic acids · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title