Method of enriching for pancreatic endocrine cells that express chromogranin A

Patent metadata

Field	Value
Publication number	US-10266808-B2
Application number	US-201615338149-A
Country	US
Kind code	B2
Filing date	Oct 28, 2016
Priority date	Apr 21, 2008
Publication date	Apr 23, 2019
Grant date	Apr 23, 2019

Abstract

The present disclosure relates to compositions and methods comprising cell surface markers for hES-derived cells, in particular, endoderm lineage cells including pancreatic endoderm-type cells, derived from hES cells.

First claim

What is claimed is:

1. A method of enriching for pancreatic endocrine cells that express chromogranin A (CHGA), the method comprising:
a) exposing an in vitro population of pancreatic cells to a ligand that binds CD200, CD318, CD57, CD142, or CD340; and
b) either i) selecting cells that bind to the ligand that binds CD200, CD318, or CD57; or
ii) selecting cells that do not bind to the ligand that binds CD142 or CD340,
thereby enriching for pancreatic endocrine cells that express CHGA.

2. The method of claim 1 , wherein said ligand comprises an antibody or binding fragment thereof that specifically binds the cell-surface marker.

3. The method of claim 1 , wherein said ligand is associated with a detectable label.

4. The method of claim 1 , wherein said ligand is associated with a magnetic particle.

5. The method of claim 1 , wherein the selecting is performed by Fluorescence Activated Cell Sorting (FACS).

6. The method of claim 1 , comprising selecting cells that bind to the ligand that binds CD318, thereby enriching for pancreatic endocrine cells that express CHGA and insulin.

7. A method of enriching for pancreatic endocrine cells that express chromogranin A (CHGA), the method comprising:
a) exposing an in vitro population of pancreatic cells to a ligand that binds CD200, CD318, or CD57; and
b) selecting cells that bind to the ligand that binds CD200, CD318, or CD57,
thereby enriching for pancreatic endocrine cells that express CHGA.

8. A method of enriching for pancreatic endocrine cells that express chromogranin A (CHGA), the method comprising:
a) exposing an in vitro population of pancreatic cells to a ligand that binds CD142, or CD340; and
b) selecting cells that do not bind to the ligand that binds CD142 or CD340,
thereby enriching for pancreatic endocrine cells that express CHGA.

Assignees

• Viacyte Inc

Inventors

• Kelly Olivia
• Bang Anne

Classifications

• C12N5/0678 Primary

  Stem cells; Progenitor cells; Precursor cells · CPC title

• C12N2506/02

  from embryonic cells · CPC title

• C12N2501/599

  with CD designations not provided for elsewhere · CPC title

• C12N2501/50

  Cell markers; Cell surface determinants · CPC title

• G01N33/68

  involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)} · CPC title