Micro titre plate

Patent metadata

Field	Value
Publication number	US-10265697-B2
Application number	US-201615292093-A
Country	US
Kind code	B2
Filing date	Oct 12, 2016
Priority date	Oct 15, 2008
Publication date	Apr 23, 2019
Grant date	Apr 23, 2019

Abstract

A micro titre plate comprising a number of wells filled with separation matrix. According to the invention the volume of the separation matrix is varied between at least some of the wells.

First claim

What is claimed is:

1. A micro titre plate comprising a number of wells disposed in a plurality of columns and rows, the wells being filled with the same type of separation matrix,
wherein the separation matrix in the wells of the plate has a series of volumes V 1 , V 2 , . . . V n distributed according to a specific pattern that does not obey logarithmic or a single degree freedom linear relationship and same separation matrix volume is provided in all the wells of either the same column or the same row in the plate, and
wherein when same volume and concentration of a liquid sample is added to each of the wells, at equilibrium, the concentration of liquid phase in one well divided by the concentration of liquid phase in a neighbouring well with different amount of separation matrix is between 1 and 3.

2. A method of filling micro titre plate of claim 1 with separation matrix, the method comprising,
determining the specific distribution pattern of volumes of separation matrix between the wells based on the specific screening process that will be performed; and
filling the wells of the micro titre plate with separation matrix according to the determined specific distribution pattern.

3. A method for determining an adsorption isotherm comprising the steps of:
using the micro titre plate of claim 1 ;
adding the same volume and concentration of a liquid sample to each of the number of wells needed for the analysis;
waiting for a predefined period of time until at least some of the sample is adsorbed onto the separation matrix; and
measuring concentration of the sample remaining in the liquid phase and/or solid phase in at least two wells with different volumes of separation matrix.

4. The micro titre plate of claim 1 , wherein the series of volumes V 1 , V 2 , . . . V n is distributed according to a specific pattern for the calculation of an adsorption isotherm of the separation matrix such that the series of volumes results in a series of corresponding isotherm data points in the calculation of the adsorption isotherm and the volumes of the separation matrix is distributed such that at least 10% of the isotherm data points obtained being below the 50% maximum capacity levels of the adsorption isotherm.

5. The micro titre plate of claim 4 , wherein each consecutive isotherm data point is obtained at 2 times higher concentration than the preceding point.

6. The micro titre plate of claim 1 , wherein each well in the plate has a permeable filter in the bottom.

7. The micro titre plate of claim 1 , wherein the series of volumes V 1 , V 2 , . . . V n of the separation matrix is distributed according to FIG. 1 and FIG. 4 .

8. The micro titre plate of claim 1 , wherein the series of volumes V 1 , V 2 , . . . V n of the separation matrix is distributed according to FIG. 2 and FIG. 4 .

9. The micro titre plate of claim 1 , wherein the series of volumes V 1 , V 2 , . . . V n of the separation matrix is distributed according to FIG. 3 and FIG. 4 .

10. The method of claim 3 , wherein the concentration of liquid phase in one well divided with the concentration of liquid phase in a neighbouring well with different amount of separation matrix is between 1 and 3.

11. The method of claim 3 , wherein the concentration of liquid phase in one well divided with the concentration of liquid phase in a neighbouring well with different amount of separation matrix is about 2.

12. The method of claim 3 , wherein the predetermined waiting time is until an equilibrium between the liquid and solid phases has been reached.

Assignees

• Ge Healthcare Bioprocess R&D Ab

Inventors

• Lacki Karol

Classifications

• G01N30/466

  with separation columns in parallel · CPC title

• G01N30/6043

  in parallel · CPC title

• B01L2300/069

  Absorbents; Gels to retain a fluid · CPC title

• B01L3/50255 Primary

  Multi-well filtration · CPC title

• Y10T436/25

  including sample preparation · CPC title