Chromatographic materials

Patent metadata

Field	Value
Publication number	US-10265679-B2
Application number	US-201815911630-A
Country	US
Kind code	B2
Filing date	Mar 5, 2018
Priority date	May 15, 2012
Publication date	Apr 23, 2019
Grant date	Apr 23, 2019

Abstract

In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W)a(Q)b(T)c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05≤(b/c)≤100, and a≥0.

First claim

The invention claimed is:

1. A chromatographic stationary phase material for normal phase chromatography, high-pressure liquid chromatography, solvated gas chromatography, supercritical fluid chromatography, sub-critical fluid chromatography, carbon dioxide based chromatography, hydrophilic interaction liquid chromatography or hydrophobic interaction liquid chromatography represented by Formula I:
[X](W) a (Q) b (T) c   Formula I

wherein:
X is a chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material, or a group of block copolymers thereof;
W is an hydroxyl group on the surface of X;
Q is represented by:

T is represented by one of:

wherein each Z group independently comprises:
a) a surface attachment group having the formula (B 1 ), x (R 5 y (R 6 ) z Si—
wherein x is an integer from 1-3,
y is an integer from 0-2,
z is an integer from 0-2,
and x+y+z=3
each occurrence of R 5 and R 6 independently represents methyl, ethyl, n-butyl, iso-butyl, tert-butyl, iso-propyl, substituted or unsubstituted aryl, cyclic alkyl, branched alkyl, lower alkyl, a protected or deprotected alcohol, or a zwitterion group; and
B 1 represents a siloxane bond;

b) an attachment to a surface organofunctional hybrid group selected from the group consisting of a direct carbon-carbon bond, a heteroatom linkage, ester linkage, ether linkage, thioether linkage, amine linkage, amide linkage, imide linkage, urea linkage, carbonate linkage, carbamate linkage, heterocycle linkage, triazole linkage and urethane linkage; or
c) an adsorbed, surface group that is not covalently attached to the surface of X; and b and c are positive numbers, 0.05≤(b/c)≤100, and a ≥0.

2. The chromatographic stationary phase material of claim 1 , wherein T is represented by:

3. The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material is in the form of a plurality of particles.

4. The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material is in the form of a monolith.

5. The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material is in the form of a superficially porous material.

6. The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has chromatographically enhancing pore geometry.

7. The chromatographic stationary phase material of claim 3 , wherein the plurality of particles have sizes between about 1.5 and 5 microns.

8. The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has a surface area of about 25 to 1100 m 2 /g.

9. The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has a pore volume of about 0.2 to 2.0 cm 3 /g.

10. The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has a micropore surface area of less than about 105 m 2 /g.

11. The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has an average pore diameter of about 20 to 1500 Å.

12. The chromatographic stationary phase material of claim 1 , wherein Q has a combined surface coverage of ≥0.5 μmol/m 2 .

13. The chromatographic stationary phase material of claim 1 , wherein Q has a combined surface coverage of ≥1.5 μmol/m 2 .

14. The chromatographic stationary phase material of claim 1 , wherein the chromatographic core material consists essentially of an inorganic material with a hybrid surrounding layer.

15. The chromatographic stationary phase material of claim 1 , wherein the chromatographic core material consists essentially of a hybrid material with an inorganic surface layer.

16. The chromatographic stationary phase material of claim 1 , wherein the chromatographic core material consists essentially of a hybrid material with a different hybrid surface layer.

Assignees

• Waters Technologies Corp

Inventors

• Wyndham Kevin D
• Morris Michael F
• Brousmiche Darryl W
• Hill Jason F
• Fairchild Jacob N

Classifications

• B01J20/28004

  Sorbent size or size distribution, e.g. particle size · CPC title

• B01J20/284

  based on alumina · CPC title

• B01D15/40

  using supercritical fluid as mobile phase or eluent · CPC title

• B01J20/28071

  being less than 0.5 ml/g · CPC title

• B01J20/32

  Impregnating or coating {; Solid sorbent compositions obtained from processes involving impregnating or coating} · CPC title