Heterotrophically cultivated recombinant microalgae

Patent metadata

Field	Value
Publication number	US-10260076-B2
Application number	US-201715443209-A
Country	US
Kind code	B2
Filing date	Feb 27, 2017
Priority date	Nov 28, 2008
Publication date	Apr 16, 2019
Grant date	Apr 16, 2019

Abstract

Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.

First claim

What is claimed is:

1. A microalgal host cell comprising recombinant nucleic acids encoding a Prototheca nitrogen responsive promoter and a heterologous gene encoding a fatty acid synthetic pathway protein, wherein the Prototheca nitrogen responsive promoter is operably linked to the coding sequence of the heterologous gene, and wherein the Prototheca nitrogen responsive promoter comprises the polynucleotide sequence of SEQ ID NO: 96.

2. The microalgal host cell of claim 1 , wherein the fatty acid synthetic pathway protein is selected from the group consisting of fatty acyl-ACP thioesterase, acyl carrier protein, ω-3 fatty acid desaturase, and ω-6-oleate desaturase.

3. The microalgal host cell of claim 1 , wherein the host cell is of the genus Prototheca.

4. The microalgal cell of claim 3 , wherein the host cell is of the species Prototheca moriformis.

5. A polynucleotide comprising nucleic acids encoding a Prototheca nitrogen responsive promoter and a heterologous gene encoding a fatty acid synthetic pathway protein, wherein the Prototheca nitrogen responsive promoter is operably linked to the coding sequence of the heterologous gene, wherein the Prototheca nitrogen responsive promoter comprises the polynucleotide sequence of SEQ ID NO: 96.

6. The polynucleotide of claim 5 , wherein the fatty acid synthetic pathway protein is selected from the group consisting of fatty acyl-ACP thioesterase, acyl carrier protein, ω-3 fatty acid desaturase, and ω-6-oleate desaturase.

7. A method of heterotrophically cultivating the microalgal host cell of claim 1 in a fermentation medium, the method comprising the steps of:
a. providing the microgalal host cell;
b. adding a fixed carbon source to the fermentation medium; and
c. cultivating the microalgal host cell.

8. The method of claim 7 , wherein the fermentation medium is a nutrient limiting fermentation medium.

Assignees

• Corbion Biotech Inc

Inventors

• Franklin Scott
• Somanchi Aravind
• Espina Karen
• Rudenko George N
• Chua Penelope

Classifications

• C07K2319/01

  containing a localisation/targetting motif · CPC title

• C12P7/64

  Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats · CPC title

• Y02T50/678

  Aviation using fuels of non-fossil origin · CPC title

• C12N9/16 Primary

  acting on ester bonds (3.1) · CPC title

• C12P7/20

  Glycerol · CPC title