Paramagnetic supports for use as assay reagents

Patent metadata

Field	Value
Publication number	US-10259969-B2
Application number	US-201816110372-A
Country	US
Kind code	B2
Filing date	Aug 23, 2018
Priority date	Jan 31, 2014
Publication date	Apr 16, 2019
Grant date	Apr 16, 2019

Abstract

A composition for use as an assay reagent includes a paramagnetic solid support comprising a coating of a synthetic copolymer. The synthetic copolymer comprises two or three of a first copolymerized monomer, a second copolymerized monomer and a third copolymerized monomer and further comprises a polyethylenic backbone.

First claim

What is claimed is:

1. A method of determining in a sample the presence and/or amount of an analyte, the method comprising:
(a) providing in combination in a medium:
(i) the sample,
(ii) a member of a signal producing system (A) bound to a member of a specific binding pair that specifically binds to the analyte or (B) bound to an analyte analog, and
(iii) a paramagnetic metal oxide solid support associated with a member of a specific binding pair that specifically binds to the analyte or associated with a member of a specific binding pair that is bound to a member of a specific binding pair member that specifically binds to the analyte;
wherein the paramagnetic metal oxide solid support comprising a coating of a synthetic copolymer wherein the synthetic copolymer comprises three of a first copolymerized monomer, a second copolymerized monomer and a third copolymerized monomer and wherein the synthetic copolymer comprises a polyethylenic backbone and wherein:
(i) the first copolymerized monomer comprises a pendant moiety of the formula: —C(O)—O—(CH 2 CH 2 O) m —Z, wherein:
Z is H or alkyl of from 1 to 6 carbon atoms,
m is 1 to 100;
(ii) the second copolymerized monomer comprises a pendant moiety of the formula:
-OC(O)NR-J wherein R is H or alkyl of from 1 to 6 carbon atoms and J is the member of a specific binding pair; and
(iii) the third copolymerized monomer comprises a pendant moiety of the formula: —COOH or a derivative thereof,

(b) subjecting the combination to conditions for forming a complex,
(c) separating the solid support from the medium, and
(d) activating the member of the signal producing system bound to the separated solid support and detecting the amount of the complex, the amount of the complex being related to the presence and/or amount of analyte in the sample.

2. A method of determining in a sample the presence and/or amount of an analyte, the method comprising:
(a) providing in combination in a medium:
(i) the sample,
(ii) a member of a signal producing system bound to a member of a specific binding pair that specifically binds to the analyte or bound to an analyte analog, and
(iii) a composition comprising:
a paramagnetic particle comprising:
(A) a member of a specific binding pair that specifically binds to the analyte or a member of a specific binding pair that is bound to a member of a specific binding pair member that specifically binds to the analyte, and
(B) a coating of a synthetic copolymer wherein the synthetic copolymer comprises three of a first copolymerized monomer, a second copolymerized monomer and a third copolymerized monomer and wherein the synthetic copolymer comprises a polyethylenic backbone and wherein:
(i) the first copolymerized monomer comprises a pendant moiety of the formula: —C(O)—O—(CH 2 CH 2 O) m —Z, wherein:

Z is H or alkyl of from 1 to 6 carbon atoms,
m is 1 to 100;
(ii) the second copolymerized monomer comprises a pendant moiety of the formula:
—OC(O)NR-J wherein R is H or alkyl of from 1 to 6 carbon atoms and J is the member of a specific binding pair; and
(iii) the third copolymerized monomer comprises a pendant moiety of the formula: —COOH or a derivative thereof,

(b) subjecting the combination to conditions for forming a complex,
(c) separating the solid support from the medium, and
(d) activating the member of the signal producing system bound to the separated solid support and detecting the amount of the complex, the amount of the complex being related to the presence and/or amount of analyte in the sample.

3. The method according to claim 2 wherein the member of the signal producing system is selected from the group consisting of chemiluminescent compounds, fluorescent compounds, sensitizers, phosphorescent compounds, dyes and enzymes.

4. The method according to claim 2 wherein the member of the signal producing system is an acridinium ester.

5. The method according to claim 2 wherein the particle is a metal oxide particle wherein the metal is a paramagnetic metal selected from the group consisting of iron, lithium, sodium, magnesium, aluminum, manganese, strontium, zirconium, molybdenum, ruthenium, rhodium, palladium, tin, samarium, europium, tungsten, and platinum.

6. The method according to claim 2 wherein:
The pendant moiety of the first copolymerized monomer is —C(O)—O—(CH 2 CH 2 O) m —Z, wherein:
Z is H or methyl and m is 1 to 100.

Assignees

• Siemens Healthcare Diagnostics Inc

Inventors

• Lele Bhalchandra S
• Allgaier Eric
• Pervere Robert
• Lyons Jeremy
• St-Onge Steve

Classifications

• C08F2/06

  Organic solvent · CPC title

• C07C233/20

  having the carbon atom of the carboxamide group bound to a carbon atom of an acyclic unsaturated carbon skeleton · CPC title

• C09D123/0869

  Acids or derivatives thereof · CPC title

• C09D133/24

  Homopolymers or copolymers of amides or imides · CPC title

• G01N33/82

  involving vitamins {or their receptors} · CPC title