Method of making a device for generating droplets

Patent metadata

Field	Value
Publication number	US-10258989-B2
Application number	US-201615365888-A
Country	US
Kind code	B2
Filing date	Nov 30, 2016
Priority date	Sep 23, 2008
Publication date	Apr 16, 2019
Grant date	Apr 16, 2019

Abstract

Methods of making a droplet-generating device. In an exemplary method, an upper member is injection molded. The upper member includes a bottom surface and also includes a first microfluidic channel, a second microfluidic channel, and a third microfluidic channel each formed in the bottom surface. The upper member has a plurality of openings each extending completely through the upper member from the bottom surface and creating a side wall region of a sample well, a carrier well, and a droplet well. A cover layer is attached to the bottom surface of the upper member, such that the cover layer seals a bottom side of each microfluidic channel. The microfluidic channels meet one another to create a droplet-generation region. The sample well, the carrier well, and the droplet well are connected to the droplet-generation region via the first, second, and third microfluidic channels, respectively.

First claim

We claim:

1. A method of making a droplet-generating device, the method comprising:
injection molding an upper member including a bottom surface and also including a first microfluidic channel, a second microfluidic channel, and a third microfluidic channel each formed in the bottom surface and arranged parallel thereto, the upper member having a plurality of openings each extending completely through the upper member from the bottom surface, the openings creating a side wall region of a sample well, a carrier well, and a droplet well; and
attaching a cover layer to the bottom surface of the upper member, such that the cover layer seals a bottom side of each microfluidic channel;
wherein the microfluidic channels meet one another to create a droplet-generation region, and wherein the sample well is connected to the droplet-generation region via the first microfluidic channel, the carrier well is connected to the droplet-generation region via the second microfluidic channel, and the droplet well is connected to the droplet-generation region via the third microfluidic channel.

2. The method of claim 1 , wherein the upper member is injection molded to include a base portion that provides the bottom surface of the upper member, and also to include a plurality of protrusions projecting upwardly from the base portion, and wherein each well has an upper portion formed by one of the protrusions.

3. The method of claim 2 , wherein the upper member is injection molded such that each protrusion is annular.

4. The method of claim 2 , wherein the cover layer is thinner than the base portion of the upper member.

5. The method of claim 1 , wherein the cover layer is thinner than the upper member.

6. The method of claim 1 , wherein each opening of the plurality of openings extends from a top side of the upper member to the bottom surface, and narrows at a position intermediate the top side and the bottom surface.

7. The method of claim 1 , wherein the upper member is injection molded to have a fourth microfluidic channel formed in the bottom surface and arranged parallel thereto, wherein the second microfluidic channel and the fourth microfluidic channel extend separately to the droplet-generation region, and wherein the carrier well also is connected to the droplet-generation region via the fourth microfluidic channel.

8. A method of making a droplet-generating device, the method comprising:
injection molding an upper member as a single piece, the upper member having a base portion and also having a first microfluidic channel, a second microfluidic channel, and a third microfluidic channel each formed in a bottom surface of the base portion, the upper member including a plurality of protrusions projecting upwardly from the base portion, wherein one of the protrusions defines an upper portion of a sample well, another one of the protrusions defines an upper portion of a carrier well, and still another one of the protrusions defines an upper portion of a droplet well; and
attaching a cover layer to the bottom surface of the base portion, wherein the cover layer seals a bottom side of each microfluidic channel;
wherein the microfluidic channels meet one another in a droplet-generation region, and wherein the sample well is configured to supply sample-containing fluid to the droplet-generation region via the first microfluidic channel, the carrier well is configured to supply carrier fluid to the droplet-generation region via the second microfluidic channel, and the droplet well is configured to receive sample-containing droplets and carrier fluid from the droplet-generation region via the third microfluidic channel.

9. The method of claim 8 , wherein the cover layer is thinner than the base portion.

10. The method of claim 8 , wherein the upper member defines a respective opening corresponding to each well, wherein the opening extends completely through the upper member from a top side to the bottom surface of the upper member, and wherein the upper member is injection molded such that the opening is narrower at the bottom surface than the top side.

11. The method of claim 8 , wherein the upper member is injection molded to have a fourth microfluidic channel formed in the bottom surface, wherein the second microfluidic channel and the fourth microfluidic channel extend separately to the droplet-generation region, and wherein the carrier well also is connected to the droplet-generation region via the fourth microfluidic channel.

Assignees

• Bio Rad Laboratories

Inventors

• Ness Kevin D
• Hindson Benjamin J
• Colston Jr Billy W
• Masquelier Donald A

Classifications

• G01N21/3563

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