Control and optimization of continuous chromatography process
US-2024295533-A1 · Sep 5, 2024 · US
US10258903B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10258903-B2 |
| Application number | US-201615542128-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 26, 2016 |
| Priority date | Mar 12, 2015 |
| Publication date | Apr 16, 2019 |
| Grant date | Apr 16, 2019 |
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A method for chromatographically separating a first saccharide from a liquid eluent comprising the first saccharide and a second saccharide by passing the liquid eluent through a bed including a polymeric macroporous alkylene-bridged resin in calcium form.
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The invention claimed is: 1. A method for chromatographically separating a first saccharide from a liquid eluent comprising the first saccharide and a second saccharide by passing the liquid eluent through a bed comprising a polymeric macroporous alkylene-bridged resin in calcium form; wherein said polymeric macroporous alkylene-bridged resin comprises pores having diameters greater than 200 Å and a macroporosity which is greater than 70% of a total pore volume. 2. The method of claim 1 wherein the resin as a surface area of from 300 to 2100 m 2 /g. 3. The method of claim 1 wherein the resin as a surface area of from 450 to 700 m 2 /g. 4. The method of claim 1 wherein the resin has a ratio of alkylene bridging to phenol of from 0.1 to 0.9:1. 5. The method of claim 1 wherein the resin has a ratio of alkylene bridging to phenol of from 0.6 to 0.8:1. 6. The method of claim 1 wherein the total pore volume of the resin is from 0.75 to 0.90 cm 3 /g. 7. The method of claim 1 wherein the resin is provided in bead form having a median diameter of from 100 to 1000 microns. 8. The method of claim 1 wherein at least 90 volume percent of the beads have a particle diameter from about 0.9 to about 1.1 times the volume average particle diameter. 9. The method of claim 1 wherein the first and second saccharide comprises glucose and fructose, respectively.
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