Bacillus sp. strain with antifungal, antibacterial and growth promotion activity

Patent metadata

Field	Value
Publication number	US-10258048-B2
Application number	US-201514814122-A
Country	US
Kind code	B2
Filing date	Jul 30, 2015
Priority date	Aug 14, 2012
Publication date	Apr 16, 2019
Grant date	Apr 16, 2019

Abstract

Disclosed herein is a Bacillus strain, Bacillus sp. isolate F727, that produces metabolites with pesticidal activities. Also provided are bioactive compositions and metabolites derived from cultures of Bacillus sp. isolate F727 capable of controlling pests; as well as methods of use of the strain and its metabolites for controlling pests.

First claim

What is claimed is:

1. A composition comprising:
(a) a supernatant, or cell fraction collected from Bacillus strain F727, NRRL Accession No. B-50768 fermentation, wherein the Bacillus strain F727 has pesticidal or plant growth promoting activity; and
(b) at least one of a carrier, diluent, surfactant, or adjuvant.

2. The composition according to claim 1 , further comprising a chemical or biological pesticide, or a plant growth promoting agent.

3. A method for modulating a pest infestation in a plant comprising applying an amount of a composition comprising: (a) a supernatant, or cell fraction collected from Bacillus strain F727, NRRL Accession No. B-50768 fermentation, wherein the Bacillus strain F727 has pesticidal or plant growth promoting activity; and (b) at least one of a carrier, diluent, surfactant, or adjuvant, sufficient to modulate said pest infestation to the plant and/or seeds thereof and/or substrate used for growing said plant, wherein the composition optionally comprises an additional pesticide or fertilizer.

4. The method of claim 3 , wherein the pest is a fungus or a bacterium.

5. The method of claim 3 , wherein the pest is a fungus and the pesticide is derived from Reynoutria sachalinensis.

6. The method of claim 4 , wherein the fungus is selected from the group consisting of Botrytis, Sclerotinia, Rhizotomies' , and Bipolaris.

7. A method for modulating the growth of a plant and/or germination of a seed comprising contacting said plant, its growth substrate, and/or a seed of said plant with an amount of a composition comprising: (a) a supernatant, or cell fraction collected from Bacillus strain F727, NRRL Accession No. B-50768 fermentation, wherein the Bacillus strain F727 has pesticidal or plant growth promoting activity; and (b) at least one of a carrier, diluent, surfactant, or adjuvant; and optionally with one or more substances effective to modulate growth of said plant and/or germination of said seed.

8. The method according to claim 7 , wherein the one or more substances comprise a growth promoting agent and/or a fertilizer.

9. The composition of claim 1 , wherein the Bacillus strain F727 is resistant to Kanamycin, Chloramphenicol, Ampicillin, Penicillin, Cefuroxime, Piperacillin or Tetracycline.

10. The composition of claim 1 , wherein the Bacillus strain F727 possesses alkaline phosphatase, esterase, acid phosphatase, or naphthol-AS-BI-Phosphohydrolase activities.

11. A seed comprising the composition of claim 1 .

12. A seed comprising the composition of claim 2 .

Assignees

• Marrone Bio Innovations Inc

Inventors

• Asolkar Ratnakar
• Cordova-Kreylos Ana-Lucia
• Mccort Christopher D
• Wilk Debora
• Todd Carly
• Su Hai
• MARRONE Pamela

Classifications

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• A01N65/30

  Polygonaceae [Buckwheat family], e.g. red-knees or rhubarb · CPC title

• C12R1/07

  Chemistry & Metallurgy · mapped topic

• A01N63/00

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title

• A01N63/02 Primary

  Human Necessities · mapped topic